Highly proficient gene targeting by homologous recombination in the human Pre-B cell line Nalm-6

Methods in Molecular Biology

Volumn 435, Issue , 2008, Pages 17-29

  Adachi, Noritaka ^a   Kurosawa, Aya ^a   Koyama, Hideki ^a  

^a YOKOHAMA CITY UNIVERSITY   (Japan)

Author keywords

Colony formation; Electroporation; Gene targeting; Genomic PCR; Homologous recombination; Ku86; MultiSite Gateway technology; Nalm 6; Nonhomologous end joining; Targeting vector

Indexed keywords

EID: 84934435281     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-232-8_2     Document Type: Article

References (16)

1. Capecchi, M. R. (1989) Altering the genome by homologous recombination. Science 244, 1288-1292.
2. Vasquez, K. M., Marburger, K., Intody, Z., and Wilson, J. H. (2001) Manipulating the mammalian genome by homologous recombination. Proc. Natl. Acad. Sci. USA 98, 8403-8410.
3. Yanez, R. J. and Porter, A. C. (1998) Therapeutic gene targeting. Gene Ther. 5, 149-159.
4. Iiizumi, S., Nomura, Y., So, S., et al. (2006) Simple one-week method to construct gene-targeting vectors: application to production of human knockout cell lines. Biotechniques 41, 311-316.
5. Grawunder, U., Zimmer, D., Fugmann, S., Schwarz, K., and Lieber, M. R. (1998) DNA ligase IV is essential for V(D)J recombination and DNA double-strand break repair in human precursor lymphocytes. Mol. Cell 2, 477-484.
6. So, S., Adachi, N., Lieber, M. R., and Koyama, H. (2004) Genetic interactions between BLM and DNA ligase IV in human cells. J. Biol. Chem. 279, 55, 433-55,442.
7. Uegaki, K., Adachi, N., So, S., Iiizumi, S., and Koyama, H. (2006) Heterozygous inactivation of human Ku70/Ku86 heterodimer does not affect cell growth, doublestrand break repair, or genome integrity. DNA Repair (Amst) 5, 303-311.
8. Adachi, N., So, S., Iiizumi, S., et al. (2006) The human pre-B cell line Nalm-6 is highly proficient in gene targeting by homologous recombination. DNA Cell Biol. 25, 19-24.
9. Hurwitz, R., Hozier, J., LeBien, T., et al. (1979) Characterization of a leukemic cell line of the pre-B phenotype. Int. J. Cancer 23, 174-180.
10. Wlodarska, I., Aventin, A., Ingles-Esteve, J., et al. (1997) A new subtype of pre-B acute lymphoblastic leukemia with t(5;12)(q31q33;pl2), molecularly and cytogenetically distinct from t(5;12) in chronic myelomonocytic leukemia. Blood 89, 1716-1722.
11. Filippini, G., Griffin, S., Uhr, M., et al. (1998) A novel flow cytometric method for the quantification of p53 gene expression. Cytometry 31, 180-186.
12. Puck, T. T., Cieciura, S. J., and Robinson, A. (1958) Genetics of somatic mammalian cells. III. Long-term cultivation of euploid cells from human and animal subjects. J. Exp. Med. 108, 945-956.
13. Lieber, M. R., Ma, Y., Pannicke, U., and Schwarz, K. (2003) Mechanism and regulation of human non-homologous DNA end-joining. Nat. Rev. Mol. Cell Biol. 4, 712-720.
14. Li, G., Nelsen, C., and Hendrickson, E. A. (2002) Ku86 is essential in human somatic cells. Proc. Natl. Acad. Sci. USA 99, 832-837.
15. Zhu, C., Bogue, M. A., Lim, D. S., Hasty, P., and Roth, D. B. (1996) Ku86-deficient mice exhibit severe combined immunodeficiency and defective processing of V(D)J recombination intermediates. Cell 86, 379-389.
16. Aratani, Y., Okazaki, R., and Koyama, H. (1992) End extension repair of introduced targeting vectors mediated by homologous recombination in mammalian cells. Nucleic Acids Res. 20, 4795-4801.