Inhibition of mitogenic signaling and induction of apoptosis in human bladder smooth muscle cells treated with doxazosin

Journal of Urology

Volumn 172, Issue 4 II, 2004, Pages 1662-1666

  Austin, Paul F ^a,b   Cook, Brian L ^a   Niederhoff, Robert A ^a   Manson, Scott R ^a   Coplen, Douglas E ^a   Weintraub, Steven J ^a   Bagli, Darius ^b  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b NONE

Author keywords

Adrenergic alpha antagonists; Apoptosis; Bladder; Cell division

Indexed keywords

BROXURIDINE; CYCLIN A; DEOXYURIDINE DERIVATIVE; DIMETHYL SULFOXIDE; DOXAZOSIN; RETINOBLASTOMA PROTEIN; SPASMOLYTIC AGENT;

APOPTOSIS; BLADDER MUSCLE; CELL CULTURE; CELL CYCLE; CELL PROLIFERATION; CELL STIMULATION; CONFERENCE PAPER; CONTROLLED STUDY; CYTOMETRY; DNA CONTENT; DRUG EFFECT; DRUG EFFICACY; DRUG MECHANISM; HUMAN; HUMAN CELL; INHIBITION KINETICS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; SIGNAL TRANSDUCTION; SMOOTH MUSCLE FIBER; WESTERN BLOTTING;

EID: 4544293366     PISSN: 00225347     EISSN: None     Source Type: Journal    
DOI: 10.1097/01.ju.0000138524.18870.af     Document Type: Conference Paper

References (20)

1. McConnell, J. D., Roehrborn, C. G., Bautista, O. M., Andriole, G. L., Jr., Dixon, C. M., Kusek, J. W. et al: The long-term effect of doxazosin, finasteride, and combination therapy on the clinical progression of benign prostatic hyperplasia. N Engl J Med, 349: 2387, 2003
2. Hu, Z. W., Shi, X. Y., Lin, R. Z., Chen, J. and Hoffman, B. B.: alpha1-Adrenergic receptor stimulation of mitogenesis in human vascular smooth muscle cells: role of tyrosine protein kinases and calcium in activation of mitogen-activated protein kinase. J Pharmacol Exp Ther, 290: 28, 1999
3. Xin, X., Yang, N., Eckhart, A. D. and Faber, J. E.: Alpha1D-adrenergic receptors and mitogen-activated protein kinase mediate increased protein synthesis by arterial smooth muscle. Mol Pharmacol, 51: 764, 1997
4. Kyprianou, N., Litvak, J. P., Borkowski, A., Alexander, R. and Jacobs, S. C.: Induction of prostate apoptosis by doxazosin in benign prostatic hyperplasia. J Urol, 159: 1810, 1998
5. Hu, Z. W., Shi, X. Y. and Hoffman, B. B.: Doxazosin inhibits proliferation and migration of human vascular smooth-muscle cells independent of alpha1-adrenergic receptor antagonism. J Cardiovasc Pharmacol, 31: 833, 1998
6. Chon, J. K., Borkowski, A., Partin, A. W., Isaacs, J. T., Jacobs, S. C. and Kyprianou, N.: Alpha 1-adrenoceptor antagonists terazosin and doxazosin induce prostate apoptosis without affecting cell proliferation in patients with benign prostatic hyperplasia. J Urol, 161: 2002, 1999
7. Weinberg, R. A.: The retinoblastoma protein and cell cycle control. Cell, 81: 323, 1995
8. Weintraub, S. J., Chow, K. N., Luo, R. X., Zhang, S. H., He, S. and Dean, D. C.: Mechanism of active transcriptional repression by the retinoblastoma protein. Nature, 375: 812, 1995
9. Knudsen, E. S., Buckmaster, C., Chen, T. T., Feramisco, J. R. and Wang, J. Y.: Inhibition of DNA synthesis by RB: effects on G1/S transition and S-phase progression. Genes Dev, 12:2278, 1998
10. Austin, P. F., Casale, A. J., Cain, M. P., Rink, R. C. and Weintraub, S. J.: Lipopolysaccharide and inflammatory cytokines cause an inducible nitric oxide synthase-dependent bladder smooth muscle fibrotic response. J Urol, 170: 645, 2003
11. Sherr, C. J. and Roberts, J. M.: CDK inhibitors: positive and negative regulators of G1-phase progression. Genes Dev, 13: 1501, 1999
12. Gorczyca, W.: Cytometric analyses to distinguish death processes. Endocr Relat Cancer, 6: 17, 1999
13. Kintscher, U., Wakino, S., Kim, S., Jackson, S. M., Fleck, E., Hsueh, W. A. et al: Doxazosin inhibits retinoblastoma protein phosphorylation and G(1)->S transition in human coronary smooth muscle cells. Arterioscler Thromb Vasc Biol, 20: 1216, 2000
14. Weintraub, S. J., Prater, C. A. and Dean, D. C.: Retinoblastoma protein switches the E2F site from positive to negative element. Nature, 358: 259, 1992
15. DeGregori, J.: The genetics of the E2F family of transcription factors: shared functions and unique roles. Biochim Biophys Acta, 1602: 131, 2002
16. Ahn, J. D., Morishita, R., Kaneda, Y., Kim, H. S., Chang, Y. C., Lee, K. U. et al: Novel E2F decoy oligodeoxynucleotides inhibit in vitro vascular smooth muscle cell proliferation and in vivo neointimal hyperplasia. Gene Ther, 9: 1682, 2002
17. Schulze, A., Zerfass, K., Spitkovsky, D., Middendorp, S., Berges, J., Helin, K. et al: Cell cycle regulation of the cyclin A gene promoter is mediated by a variant E2F site. Proc Natl Acad Sci USA, 92: 11264, 1995
18. Pagano, M., Pepperkok, R., Verde, F., Ansorge, W. and Draetta, G.: Cyclin A is required at two points in the human cell cycle. Embo J, 11: 961, 1992
19. Gonzalez-Juanatey, J. R., Iglesias, M. J., Alcaide, C., Pineiro, R. and Lago, F.: Doxazosin induces apoptosis in cardiomyocytes cultured in vitro by a mechanism that is independent of alpha1-adrenergic blockade. Circulation, 107: 127, 2003
20. Anglin, I. E., Glassman, D. T. and Kyprianou, N.: Induction of prostate apoptosis by alpha1-adrenoceptor antagonists: mechanistic significance of the quinazoline component. Prostate Cancer Prostatic Dis, 5: 88, 2002