Comparison of real-time PCR detection methods for B1 and P30 genes of Toxoplasma gondii

Diagnostic Microbiology and Infectious Disease

Volumn 45, Issue 4, 2003, Pages 269-271

  Buchbinder, Susanne ^a   Blatz, Rosemarie ^a   Rodloff, Arne Christian ^a  

^a UNIVERSITY OF LEIPZIG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BIOASSAY; COMPARATIVE STUDY; CONTROLLED STUDY; GENE LOCUS; GENE TARGETING; GENOME; MICROORGANISM DETECTION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; TOXOPLASMA GONDII;

EID: 0038364018     PISSN: 07328893     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0732-8893(02)00549-7     Document Type: Article

References (8)

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2. Burg J.L., et al. Direct and sensitive detection of a pathogenic protozoan. Toxoplasma gondii, by polymerase chain reaction. J Clin Microbiol. 27:1989;1787-1792.
3. Bretagne S., et al. Prospective study of Toxoplasma reactivation by polymerase chain reaction in allogeneic stem-cell transplant recipients. Trans Infect Dis. 2:2000;127-132.
4. Cornelissen A.W.C.A., et al. Determination of nuclear DNA of five cucoccidian parasites, Isospora (Toxoplasma) gondii, Sarcocystis cruzi, eimeria tenella, E. acervulina and Plasmodium berghei, with special reference to gamotogenesis and meiosis I. (T) gondii. Parasitology. 88:1984;531-553.
5. Costa J.M., et al. Real-time PCR for diagnosis and follow-up of Toxoplasma reactivation after allogeneic stem cell transplantation using fluorescence resonance energy transfer hybridization probes. J Clin Microbiol. 38:2000;2929-2932.
6. Kupferschmidt O., et al. Quantitative detection of Toxoplasma gondii DNA in human body fluids by TaqMan polymerase chain reaction. Clin Microbiol Infect. 7:2001;120-124.
7. Homan W.L., et al. Identification of a 200- to 300-fold repetitive 529 bp DNA fragment in Toxoplasma gondii, and its use for diagnostic and quantitative PCR. Int J Parasitol. 30:2000;69-75.
8. Jones C.D., et al. Comparison of PCR detection methods for B1, P30, and 18S rDNA genes of T. gondii in aqueous humor. IOVS. 41:(3):2000;634-644.