Comparison of the specificity and sensitivity of PCR, nested PCR, and real-time PCR for the diagnosis of histomoniasis

Avian Diseases

Volumn 49, Issue 3, 2005, Pages 366-370

  Hafez, H M ^a   Hauck, R ^a   Luschow D ^a   McDougald, L ^b  

^a FREIE UNIVERSITÄT BERLIN   (Germany)

^b UNIVERSITY OF GEORGIA   (United States)

Author keywords

Diagnosis; Histomonas meleagridis; PCR; Real time PCR

Indexed keywords

GALLUS GALLUS; HISTOMONAS MELEAGRIDIS; PROTOZOA;

EID: 24944508114     PISSN: 00052086     EISSN: None     Source Type: Journal    
DOI: 10.1637/7341-020805R.1     Document Type: Article

References (31)

1. Belak, S., and P. Thorén. Validation and quality control of polymerase chain reaction methods for the diagnosis of infectious diseases. In: Manual of diagnostic tests and vaccines for terrestrial animals, 5th ed. Office International des Epizooties. 2004. Available at: http://www.oie.int/ eng/normes/mmanual/A_summry.htm.
2. Boom, R., C. Sol, J. Weel, Y. Gerrits, M. de Boer, and P. Wertheimvan Dillen. A highly sensitive assay for detection and quantitation of human cytomegalovirus DNA in serum and plasma by PCR and electrochemiluminescence. J. Clin. Microbiol. 37:1489-1497. 1999.
3. Caccio, S. M., M. De Giacomo, and E. Pozio. Sequence analysis of the beta-giardin gene and development of a polymerase chain reaction-restriction fragment length polymorphism assay to genotype Giardia duodenalis cysts from human faecal samples. Int. J. Parasitol. 32:1023-1030. 2002.
4. Cattoli, G., A. Drago, S. Maniero, A. Toffan, E. Bertoli, S. Fassina, C. Terregino, C. Robbi, G. Vicenzoni, and I. Capua. Comparison of three rapid detection systems for type A influenza virus on tracheal swabs of experimentally and naturally infected birds. Avian Pathol. 33:432-437. 2004.
5. Chakrabarti, D., J. B. Dame, R. R. Gutell, and C. A. Yowell. Characterization of the rDNA unit and sequence analysis of the small subunit rRNA and 5.8S rRNA genes from Tritrichomonas foetus. Mol. Biochem. Parasitol. 52:75-83. 1992.
6. Clarkson, M. J. The progressive pathology of Heterakis-produced histomoniasis in turkeys. Res. Vet. Sci. 3:443-448. 1962.
7. Delgado-Viscogliosi, P., E. Viscogliosi, D. Gerbod, J. Kulda, M. L. Sogin, and V. P. Edgcomb. Molecular phylogeny of parabasalids based on small subunit rRNA sequences, with emphasis on trichomonadinae subfamily. J. Eukaryot. Microbiol. 47:70-75. 2000.
8. Dwyer, D. M. An improved method for cultivating Histomonas meleagridis. J. Parasitol. 56:191-192. 1970.
9. Esquenet, C., P. De Herdt, H. De Bosschere, S. Ronsmans, R. Ducatelle, and J. Van Erum. An outbreak of histomoniasis in free-range layer hens. Avian Pathol. 32:305-308. 2003.
10. Gerbod, D., V. P. Edgcomb, C. Noel, L. Zenner, R. Wintjens, P. Delgado-Viscogliosi, M. E. Holder, M. L. Sogin, and E. Viscogliosi. Phylogenetic position of the trichomonad parasite of turkeys, Histomonas meleagridis (Smith) Tyzzer, inferred from small subunit rRNA sequence. J. Eukaryot. Microbiol. 48:498-504. 2001.
11. Guy, R. A., C. Xiao, and P. A. Horgen. Real-time PCR assay for detection and genotype differentiation of Giardia lamblia in stool specimens. J. Clin. Microbiol. 42:3317-3320. 2004.
12. Hafez, H. M., A. Mazaheri, C. Prusas, K. Böhland, M. Pöppel, and D. Schulze. Aktuelle Geflügelkrankheiten bei Legehennen im Zusammenhang mit alternativen Haltungssystemen. Tierärztl Prax 29:168-174. 2001.
13. Hardick, J., S. Yang, S. Lin, D. Duncan, and C. Gaydos. Use of the Roche LightCycler instrument in a real-time PCR for Trichomonas vaginalis in urine samples from females and males. J. Clin. Microbiol. 41: 5619-5622. 2003.
14. Heid, C. A., J. Stevens, K. J. Livak, and P. M. Williams. Real time quantitative PCR. Genome Res. 6:986-994. 1996.
15. Hierl, T., U. Reischl, P. Lang, H. Hebart, M. Stark, P. Kyme, and I. B. Autenrieth. Preliminary evaluation of one conventional nested and two real-time PCR assays for the detection of Toxoplasma gondii in immunocompromised patients. J. Med. Microbiol. 53:629-632. 2004.
16. Hoffmann, T. Avipocker. - molekularbiologische Untersuchungen zum Nachweis, zur Epidemiologie und zur Differenzierung aviärer Pockenviren, in Fachbereich Veterinärzmedizin. Dr. med. ver. thesis, Free University Berlin. 2005.
17. Jordan, J. A., D. Lowery, and M. Trucco. TaqMan-based detection of Trichomonas vaginalis DNA from female genital specimens. J. Clin. Microbiol. 39:3819-3822. 2001.
18. Kemp, R. L., and W. M. Reid. Staining techniques for differential diagnosis of histomoniasis and mycosis in domestic poultry. Avian Dis. 10: 357-363. 1966.
19. Kemp, R. L., and W. M. Reid. Studies on the etiology of blackhead disease: the roles of Histomonas meleagridis and Candida albicans in the United States. Poult. Sci. 45:1296-1302. 1966.
20. Lee, M. A., C. H. Tan, L. T. Aw, C. S. Tang, M. Singh, S. H. Lee, H. P. Chia, and E. P. Yap. Real-time fluorescence-based PCR for detection of malaria parasites. J. Clin. Microbiol. 40:4343-4345. 2002.
21. Lund, E. E. Histomoniasis. In: Diseases of poultry, 6th ed. M. S. Hofstad, ed. Iowa State Press, Ames, IA. pp. 990-1006. 1972.
22. McDougald, L. Research on the epidemiology and pathogenicity of histomoniasis in chickens and turkeys. In: Turkey production: balance act between consumer protection, animal welfare and economic aspects. H. M. Hafez, ed. Verlag Ulmer, Stuttgart, Germany. pp. 42-47. 2003.
23. McDougald, L. R. Other protozoan diseases of the intestinal tract - histomoniasis (blackhead). In: Diseases of poultry, 11th ed. Y. M. Saif, ed. Iowa State Press, Ames, IA. pp. 1001-1010. 2003.
24. McDougald, L. R., and R. Galloway. Blackhead disease: in vitro isolation of Histomonas meleagridis as a potentially useful diagnostic aid. Avian Dis. 17:847-850. 1973.
25. Perandin, F., N. Manca, A. Calderaro, G. Piccolo, L. Galati, L. Ricci, M. C. Medici, M. C. Arcangeletti, G. Snounou, G. Dettori, and C. Chezzi. Development of a real-time PCR assay for detection of Plasmodium falciparum, Plasmodium vivax, and Plasmodium ovale for routine clinical diagnosis. J. Clin. Microbiol. 42:1214-1219. 2004.
26. Riley, D. E., M. C. Roberts, T. Takayama, and J. N. Krieger. Development of a polymerase chain reaction-based diagnosis of Trichomonas vaginalis. J. Clin. Microbiol. 30:465-472. 1992.
27. Rozen, S., and H. Skaletsky. Primer3 on the WWW for general users and for biologist programmers. Methods Mol. Biol. 132:365-386. 2000.
28. Smith, T. An infectious disease among turkeys caused by protozoa (infectious entero-hepatitis). Buz. Anim. Ind. USDA Bul. 8 (7). 1895
29. (cited in Hinshaw, W. T. Diseases of turkeys. In: Diseases of poultry, 5th ed. H. E. Biester and L. H. Schwarte, ed. Iowa State Press, Ames, IA. pp. 1253-1365. 1959).
30. Thompson, J. D., D. G. Higgins, and T. J. Gibson. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680. 1994.
31. van Beek, P. Histomoniasis in turkey flocks: clinical observations and some future strategies in prevention, early diagnosis and treatment. In: Turkey production: balance act between consumer protection, animal welfare and economic aspects. H. M. Hafez, ed. Verlag Ulmer, Stuttgart, Germany. pp. 28-36. 2003.