A purification method for improving the process yield and quality of recombinant human granulocyte colony-stimulating factor expressed in Escherichia coli and its characterization

Biotechnology and Applied Biochemistry

Volumn 50, Issue 2, 2008, Pages 77-87

  Rao, Dasari Venkata Krishna ^a   Narasu, Mangamoori Lakshmi ^b   Rao, Adibhatla Kali Satya Bhujanga ^a  

^a NATCO   (India)

^b JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY   (India)

Author keywords

Complete refolding; High purity; High recovery; Impurity profile; Process development; Recombinant human granulocyte colony stimulating factor (rHG CSF)

Indexed keywords

BIOMOLECULES; CHROMATOGRAPHIC ANALYSIS; COLUMN CHROMATOGRAPHY; ESCHERICHIA COLI; NUCLEIC ACIDS; POLYSACCHARIDES; SIZE EXCLUSION CHROMATOGRAPHY; SOAPS (DETERGENTS);

(E ,3E) PROCESS; BED HEIGHT; CONCENTRATION (COMPOSITION); CONTROL STRATEGIES; GRANULOCYTE-COLONY STIMULATING FACTOR (G-CSF); HOST CELLS; KEY PARAMETERS; LIPOPOLYSACCHARIDES (LPS); PORTLAND; PROCESS COSTS; PROCESS YIELD; PRODUCT YIELDS; PROTEIN REFOLDING; PURIFICATION METHODS; SIZE EXCLUSION CHROMATOGRAPHY (SEC);

PURIFICATION;

CELL PROTEIN; GRANULOCYTE COLONY STIMULATING FACTOR; LIPOPOLYSACCHARIDE; RECOMBINANT GRANULOCYTE COLONY STIMULATING FACTOR; RECOMBINANT PROTEIN;

ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; GEL FILTRATION CHROMATOGRAPHY; GEL PERMEATION CHROMATOGRAPHY; NONHUMAN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; CHEMISTRY; HUMAN; ISOLATION AND PURIFICATION; LIQUID CHROMATOGRAPHY; METABOLISM; METHODOLOGY; PHYSIOLOGY; PROTEIN ENGINEERING;

ESCHERICHIA COLI;

CHROMATOGRAPHY, LIQUID; ESCHERICHIA COLI; GRANULOCYTE COLONY STIMULATING FACTOR, RECOMBINANT; HUMANS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS;

EID: 44849086758     PISSN: 08854513     EISSN: None     Source Type: Journal    
DOI: 10.1042/BA20070130     Document Type: Article

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