Novel assays for proteases using green fluorescent protein-tagged substrate immobilized on a membrane disk

Analytical Biochemistry

Volumn 378, Issue 2, 2008, Pages 132-137

  Aoki, Takashi ^a   Tsuchida, Shirou ^a   Yahara, Taemi ^a   Hamaue, Naoya ^a  

^a HEALTH SCIENCES UNIVERSITY OF HOKKAIDO   (Japan)

Author keywords

Disk separation assay; Green fluorescent protein; Homogeneous assay; Nylon membrane; Protease; Staphylococcal protein A

Indexed keywords

FLUORESCENCE; MEMBRANES; POLYAMIDES; RAYON; SEPARATION;

GREEN FLUORESCENT PROTEIN; HOMOGENEOUS ASSAYS; NYLON MEMBRANES; PROTEASE; STAPHYLOCOCCAL PROTEIN A;

PROTEINS;

GREEN FLUORESCENT PROTEIN; NYLON; PROTEINASE K; STAPHYLOCOCCUS PROTEIN A; TRYPSIN;

ARTICLE; PRESERVATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ISOLATION; REPRODUCIBILITY; ULTRAVIOLET RADIATION;

EID: 44649129938     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.04.022     Document Type: Article

References (19)

1. Olsen M.J., Stephens D., Griffiths D., Daugherty P., Georgiou G., and Iverson B.L. Function-based isolation of novel enzymes from a large library. Nature Biotechnol. 18 (2000) 1071-1074
2. Eggeling C., Jager S., Winkler D., and Kask P. Comparison of different fluorescence fluctuation methods for their use in FRET assays: Monitoring a protease reaction. Curr. Pharm. Biotechnol. 6 (2005) 351-371
3. Sun H., Panicker R.C., and Yao S.Q. Activity based fingerprinting of proteases using FRET peptides. Biopolymers 88 (2007) 141-149
4. Bolger R., and Checovich W. A new protease activity assay using fluorescence polarization. BioTechniques 17 (1994) 585-589
5. Oh L.Y., Larsen P.H., Krekoski C.A., Edwards D.R., Donovan F., Werb Z., and Yong V.W. Matrix metalloproteinase-9/gelatinase B is required for process outgrowth by oligodendrocytes. J. Neurosci. 19 (1999) 8464-8475
6. Grys E.L., Schade S.Z., Cohen M.E., Geivelis M., Robinson P.J., and Simonson L.G. Measurement of proteases in human subgingival dental plaque by fluorescence polarization. Arch. Oral Biol. 45 (2000) 1101-1106
7. Blommel P.G., and Fox B.G. Fluorescence anisotropy assay for proteolysis of specifically labeled fusion proteins. Anal. Biochem. 336 (2005) 75-86
8. Fujino H., Aoki T., and Watabe H. A highly sensitive assay for proteases using staphylococcal protein A fused with enhanced green fluorescent protein. Biosci. Biotechnol. Biochem. 66 (2002) 1601-1604
9. Chalfie M., Tu Y., Euskirchen G., Ward W.W., and Prasher D.C. Green fluorescent protein as a marker for gene expression. Science 263 (1994) 802-805
10. Cubitt A.B., Heim R., Adams S.R., Boyd A.E., Gross L.A., and Tsien R.Y. Understanding, improving, and using green fluorescent proteins. Trends Biochem. Sci. 20 (1995) 448-455
11. Misteli T., and Spector D.L. Applications of the green fluorescent protein in cell biology and biotechnology. Nat. Biotechnol. 15 (1997) 961-964
12. Mitra R.D., Silva C.M., and Youvan D.C. Fluorescence resonance energy transfer between blue-emitting and red-shifted excitation derivatives of the green fluorescent protein. Gene 173 (1996) 13-17
13. Jones J., Heim R., Hare E., Stack J., and Pollok B.A. Development and application of a GFP-FRET intracellular caspase assay for drug screening. J. Biomol. Screen. 5 (2000) 307-318
14. Kohl T., Heinze K.G., Kuhlemann R., Koltermann A., and Schwille P. A protease assay for two-photon cross-correlation and FRET analysis based solely on fluorescent proteins. Proc. Natl. Acad. Sci. USA 99 (2002) 12161-12166
15. Hsu Y.Y., Liu Y.N., Wang W., Kao F.J., and Kung S.H. In vivo dynamics of enterovirus protease revealed by fluorescence resonance emission transfer (FRET) based on a novel FRET pair. Biochem. Biophys. Res. Commun. 353 (2007) 939-945
16. Martin S.F., Hattersley N., Samuel I.D., Hay R.T., and Michael H.T. A fluorescence-resonance-energy-transfer-based protease activity assay and its use to monitor paralog-specific small ubiquitin-like modifier processing. Anal. Biochem. 363 (2007) 83-90
17. Aoki T., Kazama H., Satoh M., Mizuki K., and Watabe H. A homogeneous assay for relative affinity of binding proteins using a green fluorescent protein tag and membrane disk. Anal. Biochem. 344 (2005) 25-32
18. 1. Biol. Pharm. Bull. 25 (2002) 168-171
19. Aoki T., Tahara T., Satoh K., Fujino H., and Watabe H. General properties of GFP-display, an electrophoretic analysis for single amino acid changes in target polypeptides. Anal. Biochem. 317 (2003) 107-115