A fluorescence-resonance-energy-transfer-based protease activity assay and its use to monitor paralog-specific small ubiquitin-like modifier processing

Analytical Biochemistry

Volumn 363, Issue 1, 2007, Pages 83-90

  Martin, Sarah F ^a   Hattersley, Neil ^b   Samuel, Ifor D W ^a   Hay, Ronald T ^b   Tatham, Michael H ^b  

^a UNIVERSITY OF ST ANDREWS   (United Kingdom)

^b UNIVERSITY OF DUNDEE   (United Kingdom)

Author keywords

C terminal hydrolase; FRET; Paralog specificity; Sentrin Protease; SUMO; Ubiquitin like modifier

Indexed keywords

ENERGY TRANSFER; HYDROLASES; LANTHANUM COMPOUNDS;

FRET; PARALOGS; SENTRIN PROTEASE; SUMO; UBIQUITIN-LIKE;

FORSTER RESONANCE ENERGY TRANSFER;

SUMO PROTEIN;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL DIFFERENTIATION; CELL FUNCTION; CELL MATURATION; ENERGY TRANSFER; ENZYME ACTIVITY; ENZYME KINETICS; EUKARYOTE; FLUORESCENCE SPECTROSCOPY; HELA CELL; HUMAN; HUMAN CELL; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS;

EUKARYOTA;

EID: 33847372862     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2006.12.018     Document Type: Article

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