Evaluation of a 2k CCD camera with an epitaxially grown CsI scintillator for recording energy-filtered electron cryo-micrographs

Journal of Electron Microscopy

Volumn 57, Issue 3, 2008, Pages 101-112

  Yasunaga, Takuo ^a,c   Wakabayashi, Takeyuki ^b  

^a KYUSHU INSTITUTE OF TECHNOLOGY   (Japan)

^b TEIKYO UNIVERSITY   (Japan)

^c JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

Catalase; CCD camera; Electron microscopy; Energy filtered imaging; Epitaxially grown CsI; Modulation transfer function

Indexed keywords

CATALASE;

ARTICLE; CHEMISTRY; CRYSTALLOGRAPHY; ELECTRON MICROSCOPY; EQUIPMENT DESIGN; IMAGE PROCESSING; INSTRUMENTATION; METHODOLOGY; SCINTILLATION CAMERA; VIDEORECORDING;

CATALASE; CRYSTALLOGRAPHY; EQUIPMENT DESIGN; GAMMA CAMERAS; IMAGE PROCESSING, COMPUTER-ASSISTED; MICROSCOPY, ELECTRON; VIDEO RECORDING;

CCD CAMERAS; CESIUM IODIDE; CHARGE COUPLED DEVICES; ELECTRONS; HYDRATION; IMAGE RESOLUTION; OPTICAL FIBERS; SCINTILLATION COUNTERS;

CATALASE; CHARGE COUPLED DEVICES CAMERA; ENERGY; ENERGY-FILTERED IMAGING; EPITAXIALLY GROWN; EPITAXIALLY GROWN CSI; HIGH SENSITIVITY; HIGH SPATIAL RESOLUTION; LOW NOISE LEVELS; SPATIAL RESOLUTION;

OPTICAL TRANSFER FUNCTION;

EID: 44649107822     PISSN: 00220744     EISSN: 14779986     Source Type: Journal    
DOI: 10.1093/jmicro/dfn006     Document Type: Article

References (26)

1. Downing K H and Hendrickson F M (1999) Performance of a 2k CCD camera designed for electron crystallography at 400 kV. Ultramicroscopy 75: 215-233.
2. Faruqi A R, Henderson R and Subramaniam (1999) Cooled CCD detector with tapered fibre optics for recording electron diffraction patterns S. Ultramicroscopy 75: 235-250.
3. Faruqi A R and Tyrell G G (1999) Evaluation of gadolinium oxysulphide (P43) phosphor used in CCD detectors for electron microscopy Ultramicroscopy 76: 69-75.
4. Ishizuka K (1993) Analysis of electron image detection efficiency of slow-scan CCD cameras. Ultramicroscopy 52: 7-20.
5. van Zwet E J and Zandberge H W (1996) Measurement of the modulation transfer function of a slow-scan CCD camera on a TEM using a thin amorphous film as test signal. Ultramicroscopy 64: 49-55.
6. Meyer R R and Kirkland A (1998) The effects of electron and photon scattering on signal and noise transfer properties of scintillators in CCD cameras used for electron detection. Ultramicroscopy 75: 23-33.
7. Faruqi A R and Subramaniam S (2000) CCD detectors in high-resolution biological electron microscopy. Q. Rev. Biophys. 33: 1-27.
8. Carragher B, Kisseberth N, Kriegman D, Milligan R A, Pottor C S, Pulokas J, and Reilein A (2000) Leginon: an automated system for acquisition of images from vitreous ice specimens. J. Struct. Biol. 132: 33-45.
9. Rose H (1989) Optimization of imaging energy filters for high-resolution analytical electron microscopy. Ultramicroscopy 28: 184-189.
10. Taya S, Taniguchi Y, Nakazawa E, and Usukura J (1996) Development of γ-type energy filtering TEM. J. Electron Microsc. 45: 307.
11. Lucic V, Forster F, and Baumeister W (2005) Structural studies by electron tomography: from cells to molecules. Annu. Rev. Biochem. 74: 833-865.
12. Sander B, Golas M M, and Stark H (2005) Advantages of CCD detectors for de novo three-dimensional structure determination in single-particle electron microscopy. J. Struct. Biol. 151: 92-105.
13. Oba K, Ito M, and Yamaguchi M, Tanaka, M. (1988) CsI(Na) scintillation plate with high spatial resolution. Adv. Electron El. Phys. 74: 247-255.
14. Gunji S, Hanada N, Hoshino T, Kamae T, Miyazaki S, Kakahashi T, Tamura T, Tanaka M, Yamagami T, Murakami H, Mori K, Tanaka H, and Yamamoto K (1990) Use of a large area photodiode in CsI(Tl) scintillation counters. Nucl. Instrum. Methods Phys. Res. Sec. A 295: 400-404.
15. Sumner J B and Dounce A L (1955) [137] Liver catalase. Meth. Enyzmol. 2: 775-781.
16. Henderson R, Baldwin J M, Ceska T A, Zemlin F, Beckmann E, and Dowing K H (1990) Model for the structure of bacteriorhodopsin based on high-resolution electron cryo-microscopy. J. Mol. Biol. 213: 899-929.
17. Yasunaga T and Wakabayashi T (1996) Extensible and object-oriented system Eos supplies a new environment for image analysis of electron micrographs of macromolecules. J. Struct. Biol. 116: 155-160.
18. Fan G Y and Ellisman M H (1996) Optimization of thin-foil based phosphor screens for CCD imaging in TEM in the voltage range of 80-400 kV. Ultramicroscopy 66: 11-19.
19. Zuo J M (1996) Electron detection characteristics of slow-scan CCD camera. Ultramicroscopy 66: 21-33.
20. Meyer R R and Kerkland A I (2000) Characterization of the signal and noise transfer of CCD cameras for electron detection. Microsc. Res. Tech. 49: 269-280.
21. Faruqi A R, Henderson R, and Tlustos L (2005) Noiseless direct detection of electrons in Medipix2 for electron microscopy. Nucl. Instr. Methods A 546: 160-163.
22. Faruqi A R (2007) Direct electron detectors for electron microscopy. Adv. Imaging Electron Phys. 145: 55-93.
23. McMullan G, Cattermole D M, Chen S, Henderson R, Llopart X, Summerfield C, Tlustos L, and Faruqi A R (2007) Electron imaging with Medipix2 hybrid pixel detector. Ultramicroscopy 107: 401-413.
24. Deptuch G, Besson A, Rehak P, Szelezniak M, Winter M, and Zhu Y (2007) Direct electron imaging in electron microscopy with monolithic active pixel sensors. Ultramicroscopy 107: 674-684.
25. Henderson R, Baldwin J M, Downing K H, Lepault J, and Zemlin F (1986) Structure of purple membrane from halobacterium halobium: recording, measurement and evaluation of electron micrographs at 3.5 Å resolution. Ultramicroscopy 19: 147-178.
26. Yonekura K, Maki S, and Namba K (2002) Quantitative comparison of zero-loss and conventional electron diffraction from two-dimensional and thin three-dimensional protein crystals. Biophys. J. 82: 2784-2797.