Expression of tropomyosin from Blattella germanica as a recombinant non-fusion protein in Pichia pastoris and comparison of its IgE reactivity with its native counterpart

Protein Expression and Purification

Volumn 37, Issue 2, 2004, Pages 273-278

  Jeong, Kyoung Yong ^a   Lee, Jongweon ^a   Lee, In Yong ^a   Hong, Chein Soo ^a   Ree, Han Il ^a   Yong, Tai Soon ^a  

^a Yonsei University College of Medicine   (South Korea)

Author keywords

Allergenicity; Allergens; Blattella germanica; Tropomyosin

Indexed keywords

ALLERGEN; AMMONIUM SULFATE; HYDROXYAPATITE; IMMUNOGLOBULIN E; PROTEIN; RECOMBINANT PROTEIN; TROPOMYOSIN;

ANIMAL; ARTICLE; BIOSYNTHESIS; CHEMISTRY; CHROMATOGRAPHY; COCKROACH; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; HUMAN; IMMUNOBLOTTING; METABOLISM; PICHIA; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN BINDING; PROTEIN TERTIARY STRUCTURE; TIME;

ALLERGENS; AMMONIUM SULFATE; ANIMALS; CHROMATOGRAPHY; COCKROACHES; DURAPATITE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; HUMANS; IMMUNOBLOTTING; IMMUNOGLOBULIN E; PICHIA; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; PROTEINS; RECOMBINANT PROTEINS; TIME FACTORS; TROPOMYOSIN;

BLATTARIA; BLATTELLA GERMANICA; ESCHERICHIA COLI; INVERTEBRATA; PICHIA; PICHIA PASTORIS;

EID: 4444322125     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.06.009     Document Type: Article

References (44)

1. Platts-Mills T.A., Chapman M.D. Allergen standardization. J. Allergy Clin. Immunol. 87:1991;621-625
2. Patterson M.L., Slater J.E. Characterization and comparison of commercially available German and American cockroach allergen extracts. Clin. Exp. Allergy. 32:2002;721-727
3. Hiller R., Laffer S., Harwanegg C., Huber M., Schmidt W.M., Twardosz A., Barletta B., Becker W.M., Blaser K., Breiteneder H., Chapman M., Crameri R., Duchene M., Ferreira F., Fiebig H., Hoffmann-Sommergruber K., King T.P., Kleber-Janke T., Kurup V.P., Lehrer S.B., Lidholm J., Muller U., Pini C., Reese G., Scheiner O., Scheynius A., Shen H.D., Spitzauer S., Suck R., Swoboda I., Thomas W., Tinghino R., Van Hage-Hamsten M., Virtanen T., Kraft D., Muller M.W., Valenta R. Microarrayed allergen molecules: diagnostic gatekeepers for allergy treatment. FASEB. J. 16:2002;414-416
4. Harwanegg C., Laffer S., Hiller R., Mueller M.W., Kraft D., Spitzuer S., Valenta R. Microarrayed recombinant allergens for diagnosis of allergy. Clin. Exp. Allergy. 33:2003;7-13
5. Pomes A., Chapman M.D. Can knowledge of the molecular structure of allergens improve immunotherapy. Curr. Opin. Allergy Clin. Immunol. 1:2001;549-554
6. Rolland J.M., O'Hehir R.E. Allergen immunotherapy: current and new therapeutic strategies. Allergol. Intl. 51:2002;221-231
7. Kazemi-Shirazi L., Verena Niederberger V., Linhart B., Lidholm J., Kraft D., Valenta R. Recombinant marker allergens: diagnostic gatekeepers for the treatment of allergy. Int. Arch. Allergy Immunol. 127:2002;259-268
8. Ferreia F., Wallner M., Breiteneder H., Hartl A., Thalhamer J., Ebner C. Genetic engineering of allergens: future therapeutic products. Int. Arch. Allergy Immunol. 128:2002;171-178
9. Aki T., Kodama T. Immunochemical characterization of recombinant and native tropomyosin as a new allergen from the house dust mite, Dermatophagoides farinae. J. Allergy Clin. Immunol. 96:1995;74-83
10. Santos A.B.R., Chapman M.D., Aalberse R.C., Vailes L.D., Ferriani V.P., Oliver C., Rizzo M.C., Naspitz C.K., Arruda L.K. Cockroach allergens and asthma in Brazil: identification of tropomyosin as a major allergen with potential cross reactivity with mite and shrimp allergens. J. Allergy Clin. Immunol. 104:1999;329-337
11. Asturias J.A., Arilla M.C., Gomez-Bayon N., Martinez A., Martinez J., Palacios R. Sequencing and high level of expression in Escherichia coli of the tropomyosin allergen (Der p 10) from Dermatophagoides pteronyssinus. Biochem. Biophys. Acta. 1397:1998;27-30
12. Asturias J.A., Eraso E., Moneo I., Martinez A. Is tropomyosin an allergen in Anisakis? Allergy. 55:2000;898-899
13. Yi F.C., Cheong N., Shek P.C.L., Wang D.Y., Chua K.Y., Lee B.W. Identification of shared and unique immunoglobulin E epitopes of the highly conserved tropomyosins in Blomia tropicalis and Dermatophagoides pteronyssinus. Clin. Exp. Allergy. 32:2002;1203-1210
14. Saarne T., Kaiser L., Rasool O., Huecas S., van Hage-Hamsten M., Gafvelin G. Cloning and characterization of two IgE-binding proteins, homologous to tropomyosin and α-tubulin, from the mite Lepidoglyphus destructor. Int. Arch. Allergy Immunol. 130:2003;258-265
15. Hodges R.S., Smillie L.B. The histidine and methionine sequences of rabbit skeletal tropomyosin. Can. J. Biochem. 50:1972;312-329
16. Cho Y.J., Liu J., Hitchcock-DeGregori S.E. The amino terminus of muscle tropomyosin is a major determinant for function. J. Biol. Chem. 265:1990;538-545
17. Hilario E., Lataro R.C., Alegria M.C., Lavarda S.C.S., Ferro J.A., Bertolini M.C. High-level production of functional muscle α-tropomyosin in Pichia pastoris. Biochem. Biophys. Res. Commun. 284:2001;955-960
18. Alegria M.C., Lavarda S.C.S., Lataro R.C., Hilario E., Ferro J.A., Bertolini M.C. Conditions affecting production of functional muscle recombinant α-tropomyosin in Saccharomyces cerevisiae. Protein Expr. Purif. 30:2003;105-111
19. K.Y. Jeong, I.Y., Lee, J. Lee, H.I. Ree, C.S. Hong, T.S. Yong, Effectiveness of health education for the control of dust mites and cockroaches in Seoul, Korea, Med. Vet. Entomol. (2004) (in press)
20. Jeong K.Y., Lee J., Lee I.Y., Ree H.I., Hong C.S., Yong T.S. Allergenicity of recombinant Bla g 7, German cockroach tropomyosin. Allergy. 58:2003;1059-1063
21. Smillie L.B. Preparation and identification of α and β tropomyosins. Methods Ezymol. 85:1982;234-241
22. Arruda L.K., Vailes L.D., Benjamin D.C., Chapman M.D. Molecular cloning of German cockroach (Blattella germanica) allergens. Int. Arch. Allergy Immunol. 107:1995;295-297
23. Kozak M. Compilation and analysis of sequences upstream from the translational start PG in eukaryotic mRNAs. Nucleic Acids Res. 12:1984;857-872
24. ′ -noncoding sequences from 699 vertebrate messenger RNAs Nucleic Acids Res. 15:1987;8125-8148
25. Johansson S.G.O., Hourihane J.O.B., Bousquet J., Bruijnzeel-Koomen C., Dreborg S., Haahtela T., Kowalski M.L., Mygind N., Ring J., van Vauwenberge P., van Hage-Hamsten M., Wuthrich B. A revised nomenclature for allergy. An EAACI position statement from EAACI nomenclature task force. Allergy. 56:2001;813-824
26. Laemmli U.K. Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature. 227:1970;680-685
27. Valenta R., Kraft D. Recombinant allergen molecules: tools to study effector cell activation. Immunol. Rev. 179:2001;119-127
28. van der Veen M.J., Mulder M., Witteman A.M., van Ree R., Aalberse R.C., Jansen H.M., van der Zee J.S. False-positive skin prick test responses to commercially available dog dander extracts caused by contamination with house dust mite (Dermatophagoides pteronyssinus) allergens. J. Allergy Clin. Immunol. 98:1996;1028-1034
29. Nelson H.S., Ilke D., Buchmeier A. Studies of allergen extract stability: the effect of dilution and mixing. J. Allergy Clin. Immunol. 98:1996;382-388
30. Schneiner O., Kraft D. Basic and practical aspects of recombinant allergens. Allergy. 50:1995;384-391
31. Cereghino J.L., Cregg J.M. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol. Rev. 24:2000;45-66
32. Shakib F., Schulz O., Sewell H. A mite subversive: cleavage of CD23 and CD25 by Der p 1 enhances allergenicity. Immunol. Today. 19:1998;313-316
33. Best E.A., Stedman K.E., Bozic C.M., Hunter S.W., Vailes L., Chapman M.D., McCall C.A., McDermott M.J. A recombinant group 1 house dust mite allergen, rDer f 1, with biological activities similar to those of the native allergen. Protein Expr. Purif. 20:2000;462-471
34. Yasuhara T., Takai T., Yuuki T., Okudaira H., Okumura Y. Biologically active recombinant forms of a major house dust mite group 1 allergen Der f 1 with full activities of both cysteine protease and IgE binding. Clin. Exp. Allergy. 31:2001;116-124
35. Pomes A. Intrinsic properties of allergens and environmental exposure as determinants of allergenicity. Allergy. 57:2002;673-679
36. Heeley D.H., Watson M.H., Mak A.S., Dubord P., Smillie L.B. Effect of phosphorylation on the interaction and functional properties of rabbit striated muscle α α-tropomyosin. J. Biol. Chem. 264:1989;2424-2430
37. Parronchi P., Brugnolo F., Sampognaro S., Maggi E. Genetic and environmental factors contributing to the onset of allergic disorders. Int. Arch. Allergy Immunol. 121:2000;2-9
38. Otsu A., Shirakawa T. Genetic and environmental factors of atopy. Allergol. Intl. 51:2002;213-219
39. Pomes A., Chapman M.D., Vailes L.D., Blundell T.L., Dhanaraj V. Cockroach allergen Bla g 2: structure, function, and implications for the allergic sensitization. Am. J. Respir. Crit. Care Med. 165:2002;391-397
40. Gold D.R., Burge H.A., Carey V., Milton D.K., Platts-Mills T., Weiss S.T.I. Predictors of repeated wheeze in the first year of life. Am. J. Respir. Crit. Care Med. 160:1999;227-236
41. Sluyter R., Tovey E.R., Duffy D.L., Britton W.J. Limited genetic control of IgE responses to rye grass allergens in Australian twins. Clin. Exp. Allergy. 28:1998;322-331
42. Ferreira F., Hirtenlehner K., Jilek A., Godnik-Cvar J., Breiteneder H., Grimm R., Hoffmann-Sommergruber K., Scheiner O., Kraft D., Breitenbach M., Rheinberger H.J., Ebner C. Dissection of immunoglobulin E and T lymphocyte reactivity of isoforms of the major birch pollen allergen Bet v 1: potential use of hypoallergenic isoforms for immunotherapy. J. Exp. Med. 183:1996;599-609
43. Heald R.W., Hitchcock-DeGregori S.E. The structure of the amino terminus of tropomyosin is critical for binding to actin in the absence and presence of troponin. J. Biol. Chem. 263:1988;5254-5259
44. Reese G., Ayuso R., Lehrer S.B. Tropomyosin: an invertebrate pan-allergen. Int. Arch. Allergy Immunol. 119:1999;247-258