A recombinant group 1 house dust mite allergen, rDer f 1, with biological activities similar to those of the native allergen

Protein Expression and Purification

Volumn 20, Issue 3, 2000, Pages 462-471

  Best, Elaine A ^a   Stedman, Kim E ^a   Bozic, Cynthia M ^a   Hunter, Shirley Wu ^a   Vailes, Lisa ^b   Chapman, Martin D ^b   McCall, Catherine A ^a   McDermott, Martin J ^a  

^a HESKA CORPORATION   (United States)

^b UNIVERSITY OF VIRGINIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALLERGEN; ASTHMA; BINDING KINETICS; DERMATOPHAGOIDES FARINAE; ENZYME ACTIVITY; EXPRESSION VECTOR; IMMUNOGLOBULIN FRAGMENT; MOLECULAR WEIGHT; PICHIA PASTORIS; PROTEIN GLYCOSYLATION; PROTEINASE; SACCHAROMYCES CEREVISIAE; SIGNAL PEPTIDE;

ACARI; ASTIGMATA; DERMATOPHAGOIDES FARINAE; PICHIA PASTORIS; PYROGLYPHIDAE; SACCHAROMYCES CEREVISIAE;

EID: 0033668126     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2000.1327     Document Type: Article

References (30)

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3. House-dust-mite allergens
4. A major house dust mite allergen disrupts the immunoglobulin E network by selectively cleaving CD23: Innate protection by antiproteases
5. Mite allergens: Significance of enzymatic activity
6. Sequence analysis of cDNA coding for a major house dust mite allergen, Der f I
7. Sequence analysis of cDNA coding for a major house dust mite allergen, Der p I: Homology with cysteine proteases
8. Production of recombinant allergens and their application for immunotherapy
9. IgE and IgG binding peptides expressed from fragments of cDNA encoding the major house dust mite allergen Der p I
10. High-frequency binding of IgE to the Der p allergen expressed in yeast
11. Production of recombinant mite allergen Der fI in insect cells and characterization of products - Removal of Pro-sequence is essential to IgE-binding activity
12. Production of recombinant Der fI with the native IgE-binding activity using a baculovirus expression system
13. Gene splicing by overlap extension: Tailor-made genes using the polymerase chain reaction
14. Small vectors for expression based on dominant drug resistance with direct multicopy selection
15. Transformation
16. Improved antibiotic resistance cassettes through restriction site elimination using Pfu DNA polymerase PCR
17. Conformational stability of B cell epitopes on group I and group II Dermatophagoides spp. allergens. Effect of thermal and chemical denaturation on the binding of murine IgG and human IgE antibodies
18. Epitope mapping of two major inhalant allergens. Der p I and Der f I, from mites of the genus Dermatophagoides
19. Cleavage of structural proteins during assembly of the head of T4 bacteriophage
20. Antigen Der f I from the dust mite Dermatophagoides farinae: Structural comparison with Der p I from Dermatophagoides pteronyssinus and epitope specificity of murine IgG and human IgE antibodies
21. High prevalence of sensitization to cat allergen among Japanese children with asthma, living without cats
22. Use of T7 RNA polymerase to direct expression of cloned genes
23. Low-usage codons in Escheriehia coli, yeast, fruit fly, and primates
24. Clustering of low usage codons and ribosome movement
25. Role of the occluding loop in cathepsin B activity
26. Functional effects of polymorphisms of house dust mite allergens
27. Structural studies of a-N-acetylgalactosaminidase: Effect of glycosylation on the level of expression, secretion efficiency, and enzyme activity
28. Purification and characterization of the major allergen from Dermatophagoides pteronyssinus antigen P1
29. Characterization of the carbohydrate moiety in a purified allergen preparation from the mite Dermatophagoides farinae and its importance for allergenic activity as tested by rast-inhibition method