Expression and purification of His-tagged rat mitochondrial short-chain 3-hydroxyacyl-CoA dehydrogenase wild-type and Ser137 mutant proteins

Protein Expression and Purification

Volumn 37, Issue 2, 2004, Pages 344-351

  Liu, Xiaojun ^a   Chu, Xiusheng ^a   Yu, Wenhua ^a   Li, Pingfeng ^b   Li, Ding ^a  

^a CITY UNIVERSITY OF HONG KONG   (Hong Kong)

^b PEKING UNIVERSITY HEALTH SCIENCE CENTER   (China)

Author keywords

Oxidation; 3 Hydroxyacyl CoA dehydrogenase; Fatty acid; His tag; Mitochondria

Indexed keywords

3 HYDROXYACYL COENZYME A DEHYDROGENASE; CYSTEINE; NICKEL; OXYGEN; SERINE; THREONINE;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BIOSYNTHESIS; CHEMICAL MODEL; CHEMISTRY; CIRCULAR DICHROISM; ENZYMOLOGY; ESCHERICHIA COLI; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MITOCHONDRION; MOLECULAR CLONING; MOLECULAR GENETICS; MUTATION; PH; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; RAT; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; X RAY CRYSTALLOGRAPHY;

3-HYDROXYACYL COA DEHYDROGENASES; AMINO ACID SEQUENCE; ANIMALS; CIRCULAR DICHROISM; CLONING, MOLECULAR; CRYSTALLOGRAPHY, X-RAY; CYSTEINE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; KINETICS; MITOCHONDRIA; MODELS, CHEMICAL; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; NICKEL; OXYGEN; PLASMIDS; RATS; SEQUENCE HOMOLOGY, AMINO ACID; SERINE; THREONINE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; VECTORS;

EID: 4444240721     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.06.026     Document Type: Article

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