Foot-and-mouth disease virus Leader proteinase: Specificity at the P2 and P3 positions and comparison with other papain-like enzymes

Biochemistry

Volumn 43, Issue 36, 2004, Pages 11482-11490

  Kuehnel, Elisabeth ^a,b   Cencic, Regina ^a   Foeger, Nicole ^a,b   Skern, Tim ^a  

^a Vienna Biocenter   (Austria)

^b GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CELLULAR PROTEINASE; FOOT-AND-MOUTH DISEASES; HOST CELLS; VIRAL POLYPROTEINS;

AMINO ACIDS; CARBON; ENZYMES; MUTAGENESIS; NITROGEN; PROTEINS; SUBSTITUTION REACTIONS; SUBSTRATES; SULFUR;

DISEASES;

ALANYLASPARAGINYLLEUCYLGLYCINE; ARGINYLLEUCYLLYSINE; ARGINYLTHREONYLTHREONYLLEUCINE; ASPARTIC ACID; CATHEPSIN K; COLLAGEN; GLYCYLALANYLGLYCYLGLUTAMINE; INITIATION FACTOR 4G; LEUCYLASPARAGINYLVALYLGLYCINE; PAPAIN; PEPTIDE; PHENYLALANINE; PROLINE; PROTEIN VP4; PROTEINASE; SERYLARGINYLARGINYLSERINE; SIGNAL PEPTIDASE; UNCLASSIFIED DRUG; VIRUS PROTEIN;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; ENZYME SPECIFICITY; FOOT AND MOUTH DISEASE VIRUS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN PROCESSING; SITE DIRECTED MUTAGENESIS;

AMINO ACID SUBSTITUTION; ANIMALS; CATHEPSINS; CATTLE; COLLAGEN TYPE I; CYSTEINE ENDOPEPTIDASES; ENDOPEPTIDASES; ENZYME PRECURSORS; EUKARYOTIC INITIATION FACTOR-4G; FOOT-AND-MOUTH DISEASE VIRUS; HYDROLYSIS; MUTAGENESIS, SITE-DIRECTED; PAPAIN; PEPTIDE FRAGMENTS; PEPTIDE INITIATION FACTORS; PROTEIN ISOFORMS; SUBSTRATE SPECIFICITY; VIRUS REPLICATION;

EUKARYOTA; FOOT-AND-MOUTH DISEASE VIRUS;

EID: 4444236444     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi049340d     Document Type: Article

References (33)

1. Mason, P. W., Grubman, M. J., and Baxt, B. (2003) Molecular basis of pathogenesis of FMDV, Virus Res. 91, 9-32.
2. Strebel, K., and Beck, E. (1986) A second protease of foot-and-mouth disease virus, J. Virol. 58, 893-899.
3. Guarné, A., Torino, J., Kirchweger, K., Pfistermueller, D., Fita, I., and Skern, T. (1998) Structure of the foot-and-mouth disease virus leader protease: a papain-like fold adapted for self-processing and eIF4G recognition, EMBO J. 17, 7469-7479.
4. Berger, A., and Schechter, L. (1970) Mapping the acttive site of papain with the aid of peptide substrates and inhibitors, Philos. Trans. R. Soc. London, Ser. B 257, 249-264.
5. Kirchweger, R., Ziegler, E., Lamphear, B. J., Waters, D., Liebig, H. D., Sommergruber, W., Sobrino, F., Hohenadl, C., Blaas, D., Rhoads, R. E., and Skern, T. (1994) Foot-and-mouth disease virus leader proteinase: Purification of the Lb form and determination of its cleavage site on eIF-4γ, J. Virol. 68, 5677-5684.
6. Devaney, M. A., Vakharia, V. N., Lloyd, R. E., Ehrenfeld, E., and Grubman, M. J. (1988) Leader protein of Foot-and-Mouth disease virus is required for cleavage of the p220 component of the cap-binding protein complex, J. Virol. 62, 4407-4409.
7. Gradi, A., Foeger, N., Skern, T., and Belsham, G. (2004) Cleavage of eukaryotic translation initiation factor 4GII within foot-and-mouth disease virus-infected cells: identification of the L-protease cleavage site in vitro, J. Virol. 78, 3271-3278.
8. Glaser, W., Cencic, R., and Skern, T. (2001) Foot-and-mouth disease Leader proteinase: involvement of C-terminal residues in self-processing and cleavage of eIF4GI, J. Biol. Chem. 276, 35473-35481.
9. Foeger, N., Glaser, W., and Skern, T. (2002) Recognition of eukaryotic initiation factor 4G isoforms by picornaviral proteinases, J. Biol. Chem. 277, 44300-44309.
10. Lamphear, B. J., Yan, R., Yang, F., Waters, D., Liebig, H. D., Klump, H., Kuechler, E., Skern, T., and Rhoads, R. E. (1993) Mapping the cleavage site in protein synthesis initiation factor eIF-4γ of the 2A proteases from human Coxsackievirus and rhinovirus, J. Biol. Chem. 268, 19200-19203.
11. Turk, D., Guncar, G., Podobnik, M., and Turk, B. (1998) Revised definition of substrate binding sites of papain-like cysteine proteases, Biol. Chem. 379, 137-147.
12. Cao, X., Bergmann, I. E., Fullkrug, R., and Beck, E. (1995) Functional analysis of the two alternative translation initiation sites of foot-and-mouth disease virus, J. Virol. 69, 560-563.
13. Forss, S., Strebel, K., Beck, E., and Schaller, H. (1984) Nucleotide sequence and genome organization of foot-and-mouth disease virus, Nucleic Acids Res. 12, 6587-6601.
14. Glaser, W., and Skern, T. (2000) Extremely efficient cleavage of eIF4G by picornaviral proteinases L and 2A in vitro, FEBS Lett. 480, 151-155.
15. Dasso, M. C., and Jackson, R. J. (1989) Efficient initiation of mammalian mRNA translation at a CUG codon, Nucleic Acids Res. 17, 6485-6497.
16. Schlick, P., Kronovetr, J., Hampoelz, B., and Skern, T. (2002) Modulation of the electrostatic charge at the active site of foot-and-mouth-disease-virus leader proteinase, an unusual papain-like enzyme, Biochem. J. 363, 493-501.
17. Etchison, D., Milburn, S. C., Edery, I., Sonenberg, N., and Hershy, J. W. B. (1982) Inhibition of HeLa cell protein synthesis following poliovirus infection correlates with the proteolysis of a 220,000-dalton polypeptide associated with eukaryotic initiation factor 3 and a cap binding protein complex, J. Biol. Chem. 257, 14806-14810.
18. Byrd, M. P., Zamora, M., and Lloyd, R. E. (2002) Generation of multiple isoforms of eukaryotic translation initiation factor 4GI by use of alternate translation initiation codons, Mol. Cell. Biol. 22, 4499-4511.
19. Bradley, C. A., Padovan, J. C., Thompson, T. L., Benoit, C. A., Chait, B. T., and Rhoads, R. E. (2002) Mass spectrometric analysis of the N terminus of translational initiation factor eIF4G-1 reveals novel isoforms, J. Biol. Chem. 277, 12559-12571.
20. Lecaille, F., Choe, Y., Brandt, W., Li, Z., Craik, C. S., and Bromme, D. (2002) Selective inhibition of the collagenolytic activity of human cathepsin K by altering its S2 subsite specificity, Biochemistry 41, 8447-8454.
21. Alves, M. F., Puzer, L., Cotrin, S. S., Juliano, M. A., Juliano, L., Bromme, D., and Carmona, A. K. (2003) S3 to S3′ subsite specificity of recombinant human cathepsin K and development of selective internally quenched fluorescent substrates, Biochem. J. 373, 981-986.
22. Harris, J. L., Backes, B. J., Leonetti, F., Mahrus, S., Ellman, J. A., and Craik, C. S. (2000) Rapid and general profiling of protease specificity by using combinatorial fluorogenic substrate libraries, Proc. Natl. Acad. Sci. U.S.A. 97, 7754-7759.
23. Pauly, T. A., Sulea, T., Ammirati, M., Sivaraman, J., Danley, D. E., Griffor, M. C., Kamath, A. V., Wang, I. K., Laird, E. R., Seddon, A. P., Menard, R., Cygler, M., and Rath, V. L. (2003) Specificity determinants of human cathepsin S revealed by crystal structures of complexes, Biochemistry 42, 3203-3213.
24. Lecaille, F., Weidauer, E., Juliano, M. A., Bromme, D., and Lalmanach, G. (2003) Probing cathepsin K activity with a selective substrate spanning its active site, Biochem. J. 375, 307-312.
25. Guamé, A., Hampoelz, B., Glaser, W., Carpena, X., Tormo, J., Fita, I., and Skern, T. (2000) Structural and biochemical features distinguish the Foot-and-Mouth Disease Virus leader proteinase from other papain-like enzymes, J. Mol. Biol. 302, 1227-1240.
26. Bromme, D., Bonneau, P. R., Lachance, P., and Storer, A. C. (1994) Engineering the S2 subsite specificity of human cathepsin S to a cathepsin L- and cathepsin B-like specificity, J. Biol. Chem. 269, 30238-30242.
27. Gradi, A., Svitkin, Y. V., Sommergruber, W., Imataka, H., Morino, S., Skern, T., and Sonenberg, N. (2003) Human rhinovirus 2A proteinase cleavage sites in eukaryotic initiation factors (eIF) 4GI and eIF4GII are different, J. Virol. 77, 5026-5029.
28. Palmenberg, A. C. (1989) in Molecular aspects of picornavirus infection and detection (Semler, B. L., and Ehrenfeld, E., Eds.) pp 211-241, American Society for Microbiology, Washington, DC.
29. Berti, P. J., and Storer, A. C. (1995) Alignment/phylogeny of the papain superfamily of cysteine proteases, J. Mol. Biol. 246, 273-283.
30. Jones, T. A. (1978) A graphics model building and refinement system for macromolecules, J. Appl. Crystallogr. 11, 268-272.
31. Esnouf, R. (1997) An extensively modified version of Molscript that includes greatly enhanced colouring capacities, J. Mol. Graphics 15, 133-138.
32. Kraulis, P. J. (1991) MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures, J. Appl. Crystallogr. 24, 946-950.
33. Merrit, E., and Murphy, M. (1994) Raster3D version 2.0. A program for photorealistic molecular graphics, Acta Crystallogr. D50, 869-873.