Differential evanescence nanometry: Live-cell fluorescence measurements with 10-nm axial resolution on the plasma membrane

Biophysical Journal

Volumn 94, Issue 6, 2008, Pages 2333-2342

  Saffarian, S ^a   Kirchhausen, T ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CLATHRIN; FLUORESCENT DYE;

ANIMAL; ARTICLE; BRAIN; CALIBRATION; CATTLE; CELL MEMBRANE; CHEMISTRY; CRYOELECTRON MICROSCOPY; FLUORESCENCE; FLUORESCENCE MICROSCOPY; INSTRUMENTATION; METABOLISM; METHODOLOGY; MONTE CARLO METHOD; REPRODUCIBILITY; STATISTICAL MODEL; THERMODYNAMICS; TIME;

ANIMALS; BRAIN; CALIBRATION; CATTLE; CELL MEMBRANE; CLATHRIN; CRYOELECTRON MICROSCOPY; FLUORESCENCE; FLUORESCENT DYES; MICROSCOPY, FLUORESCENCE; MODELS, STATISTICAL; MONTE CARLO METHOD; REPRODUCIBILITY OF RESULTS; THERMODYNAMICS; TIME FACTORS;

EID: 44049089141     PISSN: 00063495     EISSN: 15420086     Source Type: Journal    
DOI: 10.1529/biophysj.107.117234     Document Type: Article

References (43)

1. Born, M. W. E. 2002. Principles of Optics. Cambridge University Press, Cambridge, UK.
2. Pawley, J. 2006. Handbook of Biological Confocal Microscopy. Springer, New York.
3. Hell, S., and E. H. K. Stelzer. 1992. Properties of a 4pi confocal fluorescence microscope. J. Opt. Soc. Am. A. 9:2159-2166.
4. Schrader, M., S. W. Hell, and H. T. M. van der Voort. 1998. Three-dimensional super-resolution with a 4Pi-confocal microscope using image restoration. J. Appl. Phys. 84:4033-4042.
5. Gustafsson, M. G. L. 2005. Nonlinear structured-illumination microscopy: wide-field fluorescence imaging with theoretically unlimited resolution. Proc. Natl. Acad. Sci. USA. 102:13081-13086.
6. Hanser, B. M., M. G. L. Gustafsson, D. A. Agard, and J. W. Sedat. 2004. Phase-retrieved pupil functions in wide-field fluorescence microscopy. J. Microsc. 216:32-48.
7. Hofmann, M., C. Eggeling, S. Jakobs, and S. W. Hell. 2005. Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins. Proc. Natl. Acad. Sci. USA. 102:17565-17569.
8. Klar, T. A., E. Engel, and S. W. Hell. 2001. Breaking Abbe's diffraction resolution limit in fluorescence microscopy with stimulated emission depletion beams of various shapes. Phys. Rev. E Stat. Nonlin. Soft Matter Phys. 64:066613.
9. Klar, T. A., S. Jakobs, M. Dyba, A. Egner, and S. W. Hell. 2000. Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission. Proc. Natl. Acad. Sci. USA. 97:8206-8210.
10. Westphal, V., and S. W. Hell. 2005. Nanoscale resolution in the focal plane of an optical microscope. Phys. Rev. Lett. 94:143903.
11. Egner, A., S. Verrier, A. Goroshkov, H. D. Soling, and S. W. Hell. 2004. 4Pi-microscopy of the Golgi apparatus in live mammalian cells. J. Struct. Biol. 147:70-76.
12. Egner, A., and S. W. Hell. 2005. Fluorescence microscopy with super- resolved optical sections. Trends Cell Biol. 15:207-215.
13. Ehrlich, M., W. Boll, A. van Oijen, R. Hariharan, K. Chandran, M. L. Nibert, and T. Kirchhausen. 2004. Endocytosis by random initiation and stabilization of clathrin-coated pits. Cell. 118:591-605.
14. Gaidarov, I., F. Santini, R. A. Warren, and J. H. Keen. 1999. Spatial control of coated-pit dynamics in living cells. Nat. Cell Biol. 1:1-7.
15. Merrifield, C. J., M. E. Feldman, L. Wan, and W. Almers. 2002. Imaging actin and dynamin recruitment during invagination of single clathrin-coated pits. Nat. Cell Biol. 4:691-698.
16. Merrifield, C. J., D. Perrais, and D. Zenisek. 2005. Coupling between clathrin-coatedpPit invagination, cortactin recruitment, and membrane scission observed in live cells. Cell. 121:593-606.
17. Giannone, G., B. J. Dubin-Thaler, O. Rossier, Y. Cai, O. Chaga, G. Jiang, W. Beaver, H.-G. Dobereiner, Y. Freund, G. Borisy, and M. P. Sheetz. 2007. Lamellipodial actin mechanically links myosin activity with adhesion-site formation. Cell. 128:561-575.
18. Ponti, A., M. Machacek, S. L. Gupton, C. M. Waterman-Storer, and G. Danuser. 2004. Two distinct actin networks drive the protrusion of migrating cells. Science. 305:1782-1786.
19. Hu, K., L. Ji, K. T. Applegate, G. Danuser, and C. M. Waterman-Storer. 2007. Differential transmission of actin motion within focal adhesions. Science. 315:111-115.
20. Morita, E., and W. I. Sundquist. 2004. Retrovirus budding. Annu. Rev. Cell Dev. Biol. 20:395-425.
21. Brown, E. L., and D. S. Lyles. 2003. Organization of the vesicular stomatitis virus glycoprotein into membrane microdomains occurs independently of intracellular viral components. J. Virol. 77:3985-3992.
22. Larson, D. R., M. C. Johnson, W. W. Webb, and V. M. Vogt. 2005. Visualization of retrovirus budding with correlated light and electron microscopy. Proc. Natl. Acad. Sci. USA. 102:15453-15458.
23. Gelles, J., B. J. Schnapp, and M. P. Sheetz. 1988. Tracking kinesin-driven movements with nanometre-scale precision. Nature. 331:450-453.
24. Thompson, R. E., D. R. Larson, and W. W. Webb. 2002. Precise nanometer localization analysis for individual fluorescent probes. Biophys. J. 82:2775-2783.
25. Betzig, E., G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess. 2006. Imaging intracellular fluorescent proteins at nanometer resolution. Science. 313:1642-1645.
26. Hess, S. T., T. P. K. Girirajan, and M. D. Mason. 2006. Ultra-high resolution imaging by fluorescence photoactivation localization microscopy. Biophys. J. 91:4258-4272.
27. Rust, M. J., M. Bates, and X. W. Zhuang. 2006. Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM). Nat. Methods. 3:793-795.
28. Peterman, E. J. G., S. Brasselet, and W. E. Moerner. 1999. The fluorescence dynamics of single molecules of green fluorescent protein. J. Phys. Chem. A. 103:10553-10560.
29. Axelrod, D. 2003. Total internal reflection fluorescence microscopy in cell biology. In Biophotonics, Part B. Academic Press, New York. 1-33.
30. Axelrod, D. 1981. Cell-substrate contacts illuminated by total internal- reflection fluorescence. J. Cell Biol. 89:141-145.
31. Sarkar, A., R. B. Robertson, and J. M. Fernandez. 2004. Simultaneous atomic force microscope and fluorescence measurements of protein unfolding using a calibrated evanescent wave. Proc. Natl. Acad. Sci. USA. 101:12882-12886.
32. Cheng, Y., W. Boll, T. Kirchhausen, S. C. Harrison, and T. Walz. 2007. Cryo-electron tomography of clathrin-coated vesicles: structural implications for coat assembly. J. Mol. Biol. 365:892-899.
33. Macia, E., M. Ehrlich, R. Massol, E. Boucrot, C. Brunner, and T. Kirchhausen. 2006. Dynasore, a cell-permeable inhibitor of dynamin. Dev. Cell. 10:839-850.
34. Massol, R. H., W. Boll, A. M. Griffin, and T. Kirchhausen. 2006. A burst of auxilin recruitment determines the onset of clathrin-coated vesicle uncoating. Proc. Natl. Acad. Sci. USA. 103:10265-10270.
35. Boucrot, E., S. Saffarian, R. Massol, T. Kirchhausen, and M. Ehrlich. 2006. Role of lipids and actin in the formation of clathrin-coated pits. Exp. Cell Res. 312:4036-4048.
36. Smith, C. J., N. Grigorieff, and B. M. F. Pearse. 1998. Clathrin coats at 21 Å resolution: a cellular assembly designed to recycle multiple membrane receptors. EMBO J. 17:4943-4953.
37. Fotin, A., Y. Cheng, P. Sliz, N. Grigorieff, S. C. Harrison, T. Kirchhausen, and T. Walz. 2004. Molecular model for a complete clathrin lattice from electron cryomicroscopy. Nature. 432:573-579.
38. Hell, S. W. 2007. Far-field optical nanoscopy. Science. 316:1153-1158.
39. Clegg, R. M. 1992. Fluorescence resonance energy transfer and nucleic acids. Methods Enzymol. 211:353-388.
40. Hickerson, R., Z. K. Majumdar, A. Baucom, R. M. Clegg, and H. F. Noller. 2005. Measurement of internal movements within the 30 S ribosomal subunit using Förster resonance energy transfer. J. Mol. Biol. 354:459-472.
41. Sund, S. E., J. A. Swanson, and D. Axelrod. 1999. Cell membrane orientation visualized by polarized total internal reflection fluorescence. Biophys. J. 77:2266-2283.
42. Churchman, L. S., Z. Okten, R. S. Rock, J. F. Dawson, and J. A. Spudich. 2005. Single molecule high-resolution colocalization of Cy3 and Cy5 attached to macromolecules measures intramolecular distances through time. Proc. Natl. Acad. Sci. USA. 102:1419-1423.
43. Rappoport, J. Z., S. Kemal, A. Benmerah, and S. M. Simon. 2006. Dynamics of clathrin and adaptor proteins during endocytosis. Am. J. Physiol. Cell Physiol. 291:C1072-C1081.