Construction and characterization of an enhanced GFP-tagged anti-BAFF scFv antibody

Applied Microbiology and Biotechnology

Volumn 79, Issue 3, 2008, Pages 423-431

  Cao, Meng ^a,c   Cao, Peng ^b   Yan, Huaijiang ^a   Ren, Fang ^a   Lu, Wuguang ^a   Hu, Yunlong ^a   Zhang, Shuangquan ^a  

^a NANJING NORMAL UNIVERSITY   (China)

^b Laboratory of Cellular and Molecular Biology Jiangsu Province Institute of Traditional Chinese Medicine   (China)

^c SOUTHEAST UNIVERSITY   (China)

Author keywords

Anti BAFF antibody; Autoimmune diseases; Bacterial expression; Enhanced green fluorescent protein (EGFP)

Indexed keywords

ELECTROPHORESIS; FLUORESCENCE; MOLECULAR WEIGHT; PATHOGENS; POLYACRYLATES; SODIUM COMPOUNDS;

ANTI-BAFF ANTIBODY; AUTOIMMUNE DISEASES; BACTERIAL EXPRESSION; ENHANCED GREEN FLUORESCENT PROTEIN (EGFP);

ANTIBODIES;

B CELL ACTIVATING FACTOR; ENHANCED GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN;

ANTIBODY; ELECTROKINESIS; GENE EXPRESSION; IMMOBILIZATION; IMMUNOASSAY; METAL BINDING; PATHOGENICITY; PROTEIN;

ARTICLE; AUTOIMMUNE DISEASE; CONFOCAL MICROSCOPY; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; HUMAN; HUMAN CELL; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; IN VITRO STUDY; MOLECULAR WEIGHT; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; WESTERN BLOTTING;

ANTIBODIES; ANTIBODY SPECIFICITY; ANTIGENS; B-CELL ACTIVATING FACTOR; CELL LINE; ENZYME-LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HUMANS; MOLECULAR WEIGHT; RECOMBINANT FUSION PROTEINS;

BACTERIA (MICROORGANISMS);

EID: 43949111629     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-008-1447-9     Document Type: Article

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