Critical role of arginine 160 of the EutB protein subunit for active site structure and radical catalysis in coenzyme B12-dependent ethanolamine ammonia-lyase

Biochemistry

Volumn 47, Issue 20, 2008, Pages 5523-5535

  Sun, Li ^a   Groover, Olivia A ^a   Canfield, Jeffrey M ^a   Warncke, Kurt ^a  

^a EMORY UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; CATALYSIS; CHAIN LENGTH; ELECTRON SPECTROSCOPY; ENZYMES; HYDROGEN BONDS; MUTAGENESIS; PARAMAGNETIC RESONANCE;

ATOM TRANSFER; ELECTRON SPIN - ECHO ENVELOPE MODULATION (ESEEM; ETHANOLAMINE AMMONIA-LYASE (EAL; HYPERFINE-COUPLED PROTEIN;

AMINO ACIDS;

ARGININE; BACTERIAL PROTEIN; COBALT DERIVATIVE; COBAMAMIDE; ETHANOLAMINE; ETHANOLAMINE AMMONIA LYASE; EUTB PROTEIN; GUANIDINE; HYDROGEN; LYSINE; MUTANT PROTEIN; NITROGEN; OLIGOMER; PROPANOLAMINE DERIVATIVE; PROTEIN SUBUNIT;

ANALYTIC METHOD; ARTICLE; CATALYSIS; CONTROLLED STUDY; ELECTRON SPIN RESONANCE; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME PURIFICATION; HYDROGEN BOND; MOLECULAR INTERACTION; NONHUMAN; PREDICTION; PRIORITY JOURNAL; PROTEIN STRUCTURE; SALMONELLA TYPHIMURIUM; SIMULATION; SITE DIRECTED MUTAGENESIS; WILD TYPE;

ARGININE; BACTERIAL PROTEINS; BINDING SITES; CATALYSIS; COBAMIDES; CRYSTALLOGRAPHY, X-RAY; ELECTRON SPIN RESONANCE SPECTROSCOPY; ENZYME ACTIVATION; ETHANOLAMINE AMMONIA-LYASE; FREE RADICALS; GUANIDINE; KINETICS; MODELS, MOLECULAR; MUTATION; PROTEIN STRUCTURE, TERTIARY; PROTEIN SUBUNITS; SALMONELLA TYPHIMURIUM; STRUCTURAL HOMOLOGY, PROTEIN; SUBSTRATE SPECIFICITY;

SALMONELLA TYPHIMURIUM;

EID: 43949084713     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi702366e     Document Type: Article

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