Expression enhancement of bubaline somatotropin in E. coli through gene modifications in the 5′-end coding region

Journal of Biotechnology

Volumn 135, Issue 2, 2008, Pages 134-139

  Sadaf, Saima ^a   Khan, Muhammad Altaf ^a   Akhtar, Muhammad Waheed ^a  

^a UNIVERSITY OF THE PUNJAB   (Pakistan)

Author keywords

Gene expression; mRNA secondary structure; PCR mutagenesis; Somatotropin; T7lac promoter; Translation initiation

Indexed keywords

ESCHERICHIA COLI; GENE ENCODING; OPTIMIZATION; PROTEINS; SUBSTITUTION REACTIONS;

MRNA SECONDARY STRUCTURE; PCR MUTAGENESIS; SOMATOTROPIN; T7LAC PROMOTERS; TRANSLATION INITIATION;

GENE EXPRESSION;

BUBALINE SOMATOTROPIN; CELL PROTEIN; GROWTH HORMONE DERIVATIVE; MESSENGER RNA;

ARTICLE; BACTERIAL CELL; CODON; CONTROLLED STUDY; DNA MODIFICATION; ESCHERICHIA COLI; GENE CONSTRUCT; GENE DELETION; GENE EXPRESSION; GENE INSERTION; GENE MUTATION; GENE SILENCING; GENETIC VARIABILITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN SECONDARY STRUCTURE; REAL TIME POLYMERASE CHAIN REACTION; RNA STABILITY; STRUCTURE ANALYSIS; SUBSTITUTION LINE;

ANIMALS; BASE SEQUENCE; BUFFALOES; CATTLE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENE EXPRESSION; GROWTH HORMONE; NUCLEIC ACID CONFORMATION; PLASMIDS; POINT MUTATION; RECOMBINANT PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER;

BUBALUS; EUKARYOTA;

EID: 43849095306     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2008.03.010     Document Type: Article

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