Immunocytochemical staining of p16ink4a protein as an adjunct test in equivocal liquid-based cytology

Diagnostic Cytopathology

Volumn 36, Issue 5, 2008, Pages 311-316

  Eun, Kyoung Shin ^a   Sa, Ra Lee ^a   Mi, Kyoung Kim ^a   Eun, Ji Kang ^a   Ju, Woong ^a   Shi, Nae Lee ^a   Woon, Sup Han ^a   Seung, Cheol Kim ^a,b  

^a Ewha Womans University School of Medicine   (South Korea)

^b Mokdong Hospital   (South Korea)

Author keywords

Adjunct test; Cervical neoplasia; Liquid based cytology; p16ink4a immunocytochemical staining

Indexed keywords

CYCLIN DEPENDENT KINASE INHIBITOR 2A; VIRUS DNA;

ADULT; AGED; ARTICLE; ATYPICAL SQUAMOUS CELL OF UNDETERMINED SIGNIFICANCE; CANCER GRADING; CANCER PATIENT; CANCER SCREENING; CONTROLLED STUDY; CYTOPATHOLOGY; DIAGNOSTIC ACCURACY; DIAGNOSTIC PROCEDURE; FEMALE; HIGH GRADE SQUAMOUS INTRAEPITHELIAL LESION; HISTOPATHOLOGY; HUMAN; HUMAN TISSUE; IMMUNOCYTOCHEMISTRY; IMMUNOREACTIVITY; LOW GRADE SQUAMOUS INTRAEPITHELIAL LESION; MAJOR CLINICAL STUDY; NONHUMAN; PRIORITY JOURNAL; UTERINE CERVIX CANCER; UTERINE CERVIX CYTOLOGY; WART VIRUS;

ADULT; AGED; ALPHAPAPILLOMAVIRUS; CARCINOMA, SQUAMOUS CELL; CERVICAL INTRAEPITHELIAL NEOPLASIA; CYCLIN-DEPENDENT KINASE INHIBITOR P16; CYTODIAGNOSIS; DNA, NEOPLASM; FEMALE; FLUORESCENT ANTIBODY TECHNIQUE, DIRECT; HUMANS; IMMUNOENZYME TECHNIQUES; MIDDLE AGED; PAPILLOMAVIRUS INFECTIONS; REPRODUCIBILITY OF RESULTS; TUMOR MARKERS, BIOLOGICAL; UTERINE CERVICAL NEOPLASMS;

EID: 43049139897     PISSN: 87551039     EISSN: 10970339     Source Type: Journal    
DOI: 10.1002/dc.20786     Document Type: Article

References (28)

1. Hausen HZ. Papillomavirus infections: A major cause of human cancers. Biochim Biophys Acta 1996;1288:F55-F78.
2. Koss LG. The Papanicolaou test for cervical cancer detection: A triumph and a tragedy. JAMA 1989;261:737-743.
3. Landis SH, Murray T, Bolden S, Wingo PA. Cancer statistics, 1999. CA Cancer J Clin 1999;49:8-31.
4. Jacobs MV, Snijders PJF, Voorhorst FJ. Reliable high-risk HPV-DNA testing by polymerase chain reaction: An intermethod and intramethod comparison. J Clin Pathol 1999;52:498-503.
5. Solomon D, Schiffman M, Tarone R. ASCUS LSIL Triage Study (ALTS) conclusions reaffirmed: Response to a November 2001 commentary. Obstet Gynecol 2002;99:671-674.
6. Wright TC Jr, Cox JT, Massad LS, Carlson J, Twiggs LB, Wilkinson EJ. 2001 ASCCP-sponsored Consensus Workshop. 2001 Consensus Guidelines for the Management of Women with Cervical Intraepithelial Neoplasia. J Low Genit Tract Dis 2003;7:154-167.
7. Mayrand MH, Duarte-Franco E, Rodrigues I, et al. Canadian cervical cancer screening trial study group human papillomavirus DNA versus Papanicolaou screening tests for cervical cancer. N Engl J Med 2007;357:1579-1588.
8. Anton M, Horky M, Blaha M. The role of tumor suppressions and viral oncoproteins in cervical carcinogenesis. Ceska Gynecol 2000;65:275-278.
9. Kato J, Mathushime H, Hiebent SW, Ewen ME, Sherr CJ. Direct binding of cyclin D to the retinoblastoma gene product (pRb) and pRb phosphorylation by the cyclin D-dependent kinase CDK4. Genes Dev 1993;7:331-342.
10. Dublin EA, Patel NK, Gillett CE, Smith P, Peters G, Barnes DM. Retinoblastoma and p16 proteins in mammary carcinoma: Their relationship to cyclin D1 and histopathological parameters. Int J Cancer 1998;79:71-75.
11. Ludlow JW, Glendening CL, Livingston DM, DeCaprio JA. Specific enzymatic dephosphorylation of the retinoblastoma protein. Mol Cell Biol 1993;13:367-372.
12. Sherr CJ. Cancer cell cycles. Science 1996;274:1672-1677.
13. Li Y, Nichols MA, Shay JW, Yiong Y. Transcriptional repression of the D-type cyclin-dependent kinase inhibitor p16 by the retinoblastoma susceptibility gene product pRb. Cancer Res 1994;54:6078-6082.
14. Khlief SN, DeGregori J, Yee CL, et al. Inhibition of cyclin D-CDK4/CDK6 activity is associated with an E2F-mediated induction of cyclin kinase inhibitor activity. Proc Natl Acad Sci USA 1996;93:4350-4354.
15. Walboomers JM, Jacobs MV, Manos MM, et al. Human papillomavirus is a necessary cause of invasive cervical cancer worldwide. J Pathol 1999;189:12-19.
16. Nakao Y, Yarg X, Yokoyama M. Induction of p16 during immortalization by HPV 16 and 18 and not during malignant transformation. Br J Cancer 1997;75:1410-1416.
17. Sano T, Oyama T, Kashiwabara K, Fukuda T, Nakajima T. Expression status of p16 protein is associated with human papillomavirus oncogenic potential in cervical and genital lesions. Am J Pathol 1998;153:1741-1748.
18. Klaes R, Friedrich T, Spitkovsky D. Overexpression of p16 (INK4A) as a specific marker for dysplastic and neoplastic epithelial cells of the cervical uteri. Int J Cancer 2001;92:276-284.
19. Bibbo M, Klump W, DeCecco J. Procedure for immunocytochemical detection of p16ink4a antigen in thin layer, liquid-based specimens. Acta Cytol 2002;46:25-29.
20. Saqi A, Pasha TL, McGrath M. Overexpression of p16ink4a in liquid-based specimens (SurePath) as marker of cervical dysplasia and neoplasia. Diagn Cytopathol 2002;27:365-370.
21. Guo M, Hu L, Baliga M, He Z, Hughson MD. The predictive value of p16ink4a and hybrid capture human papillomavirus testing for high-grade cervical intraepithelial neoplasia. Am J Clin Pathol 2004;122:894-901.
22. Nieh S, Chen SF, Chu TY, Lai HC, Fu E. Expression of p16(INK4A) in Papanicolaou smears containing atypical squamous cells of undetermined significance from the uterine cervix. Gynecol Oncol 2003;91:201-208.
23. ink4a expression more useful than human papillomavirus test to determine the outcome of atypical squamous cells of undetermined significance-categorized Pap smear? A comparative analysis using abnormal cervical smears with follow-up biopsies. Gynecol Oncol 2005;97:35-40.
24. Klaes R, Friedrich T, Spitkovsky D, et al. Overexpression of p16(INK4a) as a specific marker for dysplastic and neoplastic epithelial cells of the cervix uteri. Int J Cancer 2001;92:276-284.
25. Wright TC, Sun XW, Koulos J. Comparison of management algorithms for the evaluation of women with low-grade cytologic abnormalities. Obstet Gynecol 1995;85:202-210.
26. Lonky NM, Navarre GL, Saunders S, Sadeghi M, Wolde-Tsadik G. Low-grade Papanicolaou smears and the Bethesda system: A prospective cytohistopathologic analysis. Obstet Gynecol 1995;85:716-720.
27. Cox JT, Lorincz AT, Schiffman MH, Sherman ME, Cullen A, Kurman RJ. Human papillomavirus testing by hybrid capture appears to be useful in triaging women with a cytologic diagnosis of atypical squamous cells of undetermined significance. Am J Obstet Gynecol 1995;172:946-954.
28. Pientong C, Ekalaksananan T, Kongyingyoes B, et al. Immunocytochemical staining of p16INK4a protein from conventional Pap test and its association with human papillomavirus infection. Diagn Cytopathol 2004;31:235-242.