Conformational flexibility of a model protein upon immobilization on self-assembled monolayers

Biotechnology and Bioengineering

Volumn 100, Issue 1, 2008, Pages 19-27

  Bigdeli, Saharnaz ^a   Talasaz, Amirali H ^a,b   Ståhl, Patrik ^a   Persson, Henrik H J ^a   Ronaghi, Mostafa ^a   Davis, Ronald W ^a   Nemat Gorgani, Mohsen ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b Stanford University   (United States)

Author keywords

Allosteric effectors; Conformational flexibility; Glutamate dehydrogenase; Immobilization; Orientation; Self assembled monolayer

Indexed keywords

CONFORMATIONAL FLEXIBILITY; FLUORIMETRIC ASSAYS; GLUTAMATE DEHYDROGENASE; SPECIFIC ACTIVITY;

CATALYST ACTIVITY; ENZYME IMMOBILIZATION; SELF ASSEMBLED MONOLAYERS;

PROTEINS;

ADENOSINE DIPHOSPHATE; GLUTAMATE DEHYDROGENASE; GOLD; LEUCINE; NICOTINAMIDE ADENINE DINUCLEOTIDE;

ADSORPTION; ALLOSTERISM; ARTICLE; BIOSENSOR; CHEMICAL BINDING; CHEMICAL MODIFICATION; CONFORMATIONAL TRANSITION; COVALENT BOND; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; FLUOROMETRY; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN IMMOBILIZATION; PROTEIN MICROARRAY; PROTEIN STRUCTURE;

ADSORPTION; ANIMALS; BINDING SITES; CATTLE; COATED MATERIALS, BIOCOMPATIBLE; COMPUTER SIMULATION; ENZYME ACTIVATION; ENZYME STABILITY; ENZYMES, IMMOBILIZED; GLUTAMATE DEHYDROGENASE; GOLD; MODELS, CHEMICAL; MODELS, MOLECULAR; PROTEIN BINDING; PROTEIN CONFORMATION;

BOVINAE;

EID: 42549153762     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21724     Document Type: Article

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