Structures of bovine glutamate dehydrogenase complexes elucidate the mechanism of purine regulation

Journal of Molecular Biology

Volumn 307, Issue 2, 2001, Pages 707-720

  Smith, Thomas J ^a   Peterson, Peter E ^a   Schmidt, Timothy ^a   Fang, Jie ^b   Stanley, Charles A ^b  

^a PURDUE UNIVERSITY   (United States)

^b CHILDREN S HOSPITAL OF PHILADELPHIA   (United States)

Author keywords

Allostery; Glutamate dehydrogenase; Hyperinsulinism; Purine regulation

Indexed keywords

2 OXOGLUTARIC ACID; ADENOSINE DIPHOSPHATE; GLUTAMATE DEHYDROGENASE; GLUTAMIC ACID; GUANOSINE TRIPHOSPHATE; NICOTINAMIDE ADENINE DINUCLEOTIDE; PURINE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE;

ARTICLE; CATTLE; ENZYME ACTIVE SITE; ENZYME STRUCTURE; ESCHERICHIA COLI; HUMAN; HYPERAMMONEMIA; HYPERINSULINISM; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; STRUCTURE ANALYSIS;

ANIMALIA; BOVINAE;

EID: 0035937256     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2001.4499     Document Type: Article

References (52)

1. L-Glutamate dehydrogenases: Distribution, properties and mechanism
2. Glutamate dehydrogenases
3. Extremely thermostable glutamate dehydrogenase from the hyperthermophilic archaebacterium Pyrococcus furiosus
4. Glutamate dehydrogenase from the thermoacidophilic archaebacterium Sulfolobus solfataricus
5. The binding of oxidized coenzymes by glutamate dehydrogenase and the effects of glutarate and purine nucleotides
6. Kinetics and mechanisms of nicotinamide-nucleotide-linked dehydrogenases
7. Negative co-operativity in glutamate dehydrogenase. Involvement of the 2-position in the induction of conformational changes
8. Kinetic studies of glutamate dehydrogenase with glutamate and norvaline as substrates
9. Kinetic studies of glutamate dehydrogenase. The reductive amination of 2-oxoglutarate
10. The mechanism of glutamate dehydrogenase reaction. IV. Evidence for random and rapid binding of substrate and coenzyme in the burst phase
11. A product-inhibition study of bovine liver glutamate dehydrogenase
12. The real-time resolution of proton-related transient-state steps in an enzymatic reaction
13. Conformational flexibility in glutamate dehydrogenase. Role of water in substrate recognition and catalysis
14. Regulation De L' Activite' De La glutamate dehydrogenase par les effecteurs GTP et ADP: ETUDE par "stopped flow"
15. Effects of adenosine 5′-diphosphate on bovine glutamate dehydrogenase: Diethyl pyrocarbonate modification
16. Regulation of bovine glutamate dehydrogenase
17. Glutamic dehydrogenase I. The effect of coenzyme on the sedimentation velocity and kinetic mechanism
18. Glutamic dehydrogenase II The effect of various nucleotides on the association-disassociation and kinetic properties
19. 6-ethenoadenosine
20. The influence of ADP, GTP and L-glutamate on the binding of the reduced coenzyme to beef-liver glutamate dehydrogenase
21. The binding of oxidised coenzyme to bovine-liver glutamate dehydrogenase studied by circular-difference spectroscopy
22. The crystal structure of glutamate dehydrogenase from Clostridium symbiosum at 0.6 nm resolution
23. Subunit assembly and active site location in the structure of glutamate dehydrogenase
24. The structure of Pyrococcus furiosus glutamate dehydrogenase reveals a key role for ion-pair networks in maintaining enzyme stability at extreme temperatures
25. The structure of the thermophilic glutamate dehydrogenase from Thermococcus ANI
26. Crystal structure of glutamate dehydrogenase from the hyperthermophilic eubacterium Thermotoga maritima at 3.0 Å resolution
27. The structure of bovine glutamate dehydrogenase provides insights into the mechanism of allostery
28. Hyperinsulinism and hyperammonemia in infants with regulatory mutations of the glutamate dehydrogenase gene
29. PROCHECK: A program to check the sterochemical quality of protein structures
30. MolView: A program to analyze and display atomic structures on the Macintosh personal computer
31. Bovine liver glutamate dehydrogenase: Tentative amino acid sequence; identification of a reactive lysine; nitration of a specific tyrosine and loss of allosteric inhibition by guanosine triphosphate
32. L-Glutamate dehydrogenase
33. Glutamate dehydrogenase IV. Studies on enzyme inactivation and coenzyme binding
34. Affinity labeling of a regulatory site of bovine liver glutamate dehydrogenase
35. Identification of the lysine and tyrosine peptides labeled by 5′-p-fluorosulfonylbenzoyladenosine in the NADH inhibitory site of glutamate dehydrogenase
36. Activation of bovine liver glutamate dehydrogenase by covalent reaction of adenosine 5′-O-[S-(4-bromo-2,3-dioxobutyl)thiophosphate] with arginine-459 at an ADP regulatory site
37. Binding of reduced cofactor to glutamate dehydrogenase
38. Studies of glutamate dehydrogenase. The interaction of ADP, GTP, and NADPH in complexes with glutamate dehydrogenase
39. Effect of trinitrophenylation of specific lysyl residues on the catalytic, regulatory, and molecular properties of bovine liver glutamate dehydrogenase
40. Identification of amino acids modified by the bifunctional affinity label 5′-(p-fluorosulfonyl)benzoyl)-8-azidoadenosine in the reduced coenzyme regulatory site of bovine liver glutamate dehydrogenase
41. Isolation and identification of cysteinyl peptide labeled by 6-[(4-bromo-2,3-dioxobutyl)thio]-6-deaminoadenosine 5′-diphosphate in the reduced diphosphopyridine nucleotide inhibitory site of glutamate dehydrogenase
42. A complex effet of arsenite on the formation of a-ketoglutarate in rate liver mitochondria
43. Physiological roles of nicotinamide nucleotide transhydrogenase
44. Studies of the energy-transfer system of submitochondrial particles. I. Competition between oxidative phosphorylation and the energy-linked nicotinamide-adenine dinucleotide transhydrogenase reaction
45. Factors governing the kinetics and steady state of the mitochondrial nicotinamide nucleotide transhydrogenase system
46. DENZO
47. Processing of X-ray diffraction data collected in oscillation mode
48. Software for handling macromolecular envelopes
49. The CCP4 suite: Programs for protein crystallography
50. Halloween . . . masks and bones