A novel genotyping strategy based on allele-specific inverse PCR for rapid and reliable identification of conditional FADD knockout mice

Molecular Biotechnology

Volumn 38, Issue 2, 2008, Pages 129-135

  Dong, Xiangbai ^a   Li, Jie ^a   Li, Shufeng ^a   Zhang, Jing ^a   Hua, Zi Chun ^a  

^a NANJING UNIVERSITY   (China)

Author keywords

Allele specific inverse PCR; Conditional gene targeting; Fas associated death domain protein (FADD); Genotyping; Mice

Indexed keywords

BIOPSY; LABORATORIES; NUCLEIC ACIDS; ORGANIC ACIDS;

ALLELE-SPECIFIC INVERSE PCR; ANIMAL POPULATIONS; BIOLOGICAL LABORATORIES; CONDITIONAL GENE KNOCKOUT; CONDITIONAL GENE TARGETING; CRE/LOXP; FAS-ASSOCIATED DEATH DOMAIN PROTEIN (FADD); GENOMIC DNA; GENOTYPING; HEART DEFECTS; IN-CELL; KNOCKOUT MICE; MICE; REAL-TIME PCR; SOUTHERN BLOTTING; WILD-TYPE;

COSMIC RAYS;

CD4 ANTIGEN; CRE RECOMBINASE; FAS ASSOCIATED DEATH DOMAIN PROTEIN; GENOMIC DNA; FADD PROTEIN, MOUSE; UNCLASSIFIED DRUG;

ALLELE SPECIFIC INVERSE POLYMERASE CHAIN REACTION; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CD4+ T LYMPHOCYTE; CONTROLLED STUDY; DNA SEQUENCE; FLOW CYTOMETRY; GENOTYPE; INVERSE POLYMERASE CHAIN REACTION; KNOCKOUT GENE; LOXP SITE; MOUSE; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING; WILD TYPE; ALLELE; ANIMAL; GENETICS; METABOLISM; METHODOLOGY; MOUSE MUTANT; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; T LYMPHOCYTE; TIME;

ANIMALIA; MUS;

ALLELES; ANIMALS; BLOTTING, SOUTHERN; FAS-ASSOCIATED DEATH DOMAIN PROTEIN; GENOTYPE; MICE; MICE, KNOCKOUT; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; T-LYMPHOCYTES; TIME FACTORS;

EID: 42449126785     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12033-007-9002-y     Document Type: Article

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