Identification of targeted embryonic stem cells using long-distance PCR

BioTechniques

Volumn 23, Issue 3, 1997, Pages 376-380

  Loukianov, Evgeny V ^a   Loukianova, Tanya ^a   Periasamy, Muthu ^a  

^a UNIVERSITY OF CINCINNATI   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; EMBRYO; EMBRYO CELL; GENETIC RECOMBINATION; HUMAN; NONHUMAN; POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING; STEM CELL;

EID: 0030884484     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97233bm05     Document Type: Article

References (6)

1. Hasty, P. and A. Bradley. 1995. Gene targeting vectors for mammalian cells. p. 1-31. In A.L. Joyner (Ed.), Gene Targeting. A Practical Approach. Oxford University Press, New York.
2. Kim, H.-S. and O. Smithies. 1988. Recombinant fragment assay for gene targeting based on the polymerase chain reaction. Nucleic Acids Res. 16:8887-8903.
3. Udy, G.B. and M.J. Evans. 1994. Microplate DNA preparation. PCR screening and cell freezing for gene targeting in embryonic stem cells. BioTechniques 17:887-894.
4. Valancius, V. and O. Smithies. 1991. Testing an "in-out" targeting procedure for making subtle genomic modifications in mouse embryonic stem cells. Mol. Cell. Biol. 11:1402-1408.
5. Van der Lugt, N., E.R. Maandag, H. te Riele, P.W. Laird and A. Berns. 1991. A pgk::hprt fusion as a selectable marker for targeting of genes in mouse embryonic stem cells: disruption of the T cell receptor d-chain encoding gene. Gene 105:263-267.
6. Wurst, W.A. and L. Joyner. 1995. Production of targeted embryonic stem cell clones, p. 33-61. In A.L. Joyner (Ed.), Gene Targeting. A Practical Approach. Oxford University Press, New York.