Generation of ρ0 cells utilizing a mitochondrially targeted restriction endonuclease and comparative analyses

Nucleic Acids Research

Volumn 36, Issue 7, 2008, Pages

  Kukat, Alexandra ^a,b   Kukat, Christian ^a   Brocher, Jan ^c   Schäfer, Ingo ^a   Krohne, Georg ^c   Trounce, Ian A ^d   Villani, Gaetano ^e   Seibel, Peter ^a  

^a UNIVERSITY OF LEIPZIG   (Germany)

^b KAROLINSKA INSTITUTE   (Sweden)

^c UNIVERSITY OF WÜRZBURG   (Germany)

^d UNIVERSITY OF MELBOURNE   (Australia)

^e UNIVERSITY OF BARI   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE STRANDED DNA; ENHANCED GREEN FLUORESCENT PROTEIN; MITOCHONDRIAL DNA; RESTRICTION ENDONUCLEASE; FLUORESCENT DYE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; SIGNAL PEPTIDE; TYPE II SITE SPECIFIC DEOXYRIBONUCLEASE;

ANIMAL CELL; ARTICLE; CELL LINE; CELL ULTRASTRUCTURE; COMPARATIVE STUDY; CONFOCAL MICROSCOPY; CONTROLLED STUDY; CULTURE MEDIUM; EUKARYOTE; EXPRESSION VECTOR; FLUORESCENCE ACTIVATED CELL SORTING; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; MITOCHONDRION; MOLECULAR CLONING; MOUSE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; SOUTHERN BLOTTING; TRANSMISSION ELECTRON MICROSCOPY; ANIMAL; CHEMISTRY; GENETICS; METABOLISM; TUMOR CELL LINE; ULTRASTRUCTURE;

EUKARYOTA; RATTUS;

ANIMALS; CELL LINE; CELL LINE, TUMOR; CULTURE MEDIA; DEOXYRIBONUCLEASE ECORI; DNA, MITOCHONDRIAL; FLUORESCENT DYES; GREEN FLUORESCENT PROTEINS; HUMANS; MICE; MICROSCOPY, CONFOCAL; MICROSCOPY, ELECTRON, TRANSMISSION; MITOCHONDRIA; PROTEIN SORTING SIGNALS; RATS; RECOMBINANT FUSION PROTEINS;

EID: 42449095005     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gkn124     Document Type: Article

References (37)

1. Wallace,D.C. (2001) A mitochondrial paradigm for degenerative diseases and ageing. Novartis Found. Symp., 235, 247-263.
2. Anderson,S., Bankier,A.T., Barrell,B.G., de Bruijn,M.H., Coulson,A.R., Drouin,J., Eperon,I.C., Nierlich,D.P., Roe,B.A. et al. (1981) Sequence and organization of the human mitochondrial genome. Nature 290, 457-465.
3. King,M.P. and Attardi,G. (1989) Human cells lacking mtDNA: Repopulation with exogenous mitochondria by complementation. Science., 246 500-503.
4. Borst,P. and Grivell,L.A. (1978) The mitochondrial genome of yeast. Cell, 15, 705-723.
5. Goldring,E.S., Grossman,L.I., Krupnick,D., Cryer,D.R. and Marmur,J. (1970) The petite mutation in yeast. Loss of mitochondrial deoxyribonucleic acid during induction of petites with ethidium bromide. J. Mol. Biol., 52, 323-335.
6. Nagley,P. and Linnane,A.W. (1970) Mitochondrial DNA deficient petite mutants of yeast. Biochem. Biophys. Res. Commun., 39, 989-996.
7. Inoue,K., Ito,S., Takai,D., Soejima,A., Shisa,H., LePecq,J.B., Segal-Bendirdjian,E., Kagawa,Y. and Hayashi,J.I. (1997) Isolation of mitochondrial DNA-less mouse cell lines and their application for trapping mouse synaptosomal mitochondrial DNA with deletion mutations. J. Biol. Chem., 272, 15510-15515.
8. Inoue,K., Takai,D., Hosaka,H., Ito,S., Shitara,H., Isobe,K., LePecq,J.B., Segal-Bendirdjian,E. and Hayashi,J. (1997) Isolation and characterization of mitochondrial DNA-less lines from various mammalian cell lines by application of an anticancer drug, ditercalinium. Biochem. Biophys. Res. Commun., 239, 257-260.
9. Meyer,R.R. and Simpson,M.V. (1969) DNA biosynthesis in mitochondria. Differential inhibition of mitochondrial and nuclear DNA polymerases by the mutagenic dyes ethidium bromide and acriflavin. Biochem. Biophys. Res. Commun., 34, 238-244.
10. Nelson,I., Hanna,M.G., Wood,N.W. and Harding,A.E. (1997) Depletion of mitochondrial DNA by ddC in untransformed human cell lines. Somat. Cell Mol. Genet., 23, 287-290.
11. Newman,A.K., Rubin,R.A., Kim,S.H. and Modrich,P. (1981) DNA sequences of structural genes for Eco RI DNA restriction and modification enzymes. J. Biol. Chem., 256, 2131-2139.
12. Gregoire,M., Morais,R., Quilliam,M.A. and Gravel,D. (1984) On auxotrophy for pyrimidines of respiration-deficient chick embryo cells. Eur. J. Biochem., 142, 49-55.
13. Morais,R. and Giguere,L. (1979) On the adaptation of cultured chick embryo cells to growth in the presence of chloramphenicol. J. Cell Physiol., 101, 77-88.
14. Morais,R., Gregoire,M., Jeannotte,L. and Gravel,D. (1980) Chick embryo cells rendered respiration-deficient by chloramphenicol and ethidium bromide are auxotrophic for pyrimidines. Biochem. Biophys. Res. Commun., 94, 71-77.
15. Jakobs,B.S., van den,B.C., Dacremont,G. and Wanders,R.J. (1994) Beta-oxidation of fatty acids in cultured human skin fibroblasts devoid of the capacity for oxidative phosphorylation. Biochim. Biophys. Acta 1211, 37-43.
16. Gilkerson,R.W., Margineantu,D.H., Capaldi,R.A. and Selker,J.M. (2000) Mitochondrial DNA depletion causes morphological changes in the mitochondrial reticulum of cultured human cells. FEBS Lett., 474, 1-4.
17. Rizzuto,R., Pinton,P., Carrington,W., Fay,F.S., Fogarty,K.E., Lifshitz,L.M., Tuft,R.A. and Pozzan,T. (1998) Close contacts with the endoplasmic reticulum as determinants of mitochondrial Ca2+ responses. Science., 280, 1763-1766.
18. Hales,K.G. and Fuller,M.T. (1997) Developmentally regulated mitochondrial fusion mediated by a conserved, novel, predicted GTPase. Cell, 90, 121-129.
19. Halestrap,A.P. (1989) The regulation of the matrix volume of mammalian mitochondria in vivo and in vitro and its role in the control of mitochondrial metabolism. Biochim. Biophys. Acta, 973, 355-382.
20. Armand,R., Channon,J.Y., Kintner,J., White,K.A., Miselis,K.A., Perez,R.P. and Lewis,L.D. (2004) The e.ects of ethidium bromide induced loss of mitochondrial DNA on mitochondrial phenotype and ultrastructure in a human leukemia T-cell line (MOLT-4 cells). Toxicol. Appl. Pharmacol., 196, 68-79.
21. Herzberg,N.H., Middelkoop,E., Adorf,M., Dekker,H.L., Van Galen,M.J., Van den,B.M., Bolhuis,P.A. and van den,B.C. (1993) Mitochondria in cultured human muscle cells depleted of mitochondrial DNA. Eur. J. Cell Biol. 61, 400-408.
22. Holmuhamedov,E., Jahangir,A., Bienengraeber,M., Lewis,L.D. and Terzic,A. (2003) Deletion of mtDNA disrupts mitochondrial function and structure, but not biogenesis. Mitochondrion, 3, 13-19.
23. Morais,R., Desjardins,P., Turmel,C. and Zinkewich-Peotti,K. (1988) Development and characterization of continuous avian cell lines depleted of mitochondrial DNA. In Vitro Cell Dev. Biol., 24, 649-658.
24. Porteous,W.K., James,A.M., Sheard,P.W., Porteous,C.M., Packer,M.A., Hyslop,S.J., Melton,J.V., Pang,C.Y., Wei,Y.H. et al. (1998) Bioenergetic consequences of accumulating the common 4977-bp mitochondrial DNA deletion. Eur. J. Biochem., 257, 192-201.
25. van den Ouweland,J.M., Maechler,P., Wollheim,C.B., Attardi,G. and Maassen,J.A. (1999) Functional and morphological abnormalities of mitochondria harbouring the tRNA (Leu)(UUR) mutation in mitochondrial DNA derived from patients with maternally inherited diabetes and deafness (MIDD) and progressive kidney disease. Diabetologia., 42, 485-492.
26. Falkenberg,M., Larsson,N.G. and Gustafsson,C.M. (2007) DNA replication and transcription in mammalian mitochondria. Annu. Rev. Biochem., 76, 679-699.
27. Hudson,G. and Chinnery,P.F. (2006) Mitochondrial DNA polymerase-gamma and human disease. Hum. Mol. Genet., 15, R244-R252.
28. Copeland,W.C. (2008) Inherited mitochondrial diseases of DNA replication. Annu. Rev. Med., 59, 131-146.
29. Srivastava,S. and Moraes,C.T. (2001) Manipulating mitochondrial DNA heteroplasmy by a mitochondrially targeted restriction endonuclease. Hum. Mol. Genet., 10, 3093-3099.
30. Tanaka,M., Borgeld,H.J., Zhang,J., Muramatsu,S., Gong,J.S., Yoneda,M., Maruyama,W., Naoi,M., Ibi,T. et al. (2002) Gene therapy for mitochondrial disease by delivering restriction endonuclease SmaI into mitochondria. J. Biomed. Sci., 9, 534-541.
31. Holt,I.J., Harding,A.E. and Morgan-Hughes,J.A. (1988) Deletions of muscle mitochondrial DNA in patients with mitochondrial myopathies. Nature, 331, 717-719.
32. DeVivo,D.C., Haymond,M.W., Obert,K.A., Nelson,J.S. and Pagliara,A.S. (1979) Defective activation of the pyruvate dehydrogenase complex in subacute necrotizing encephalomyelopathy (Leigh disease). Ann. Neurol., 6, 483-494.
33. King,M.P. and Attardi,G. (1996) Isolation of human cell lines lacking mitochondrial DNA. Methods Enzymol., 264, 304-313.
34. Brooks,G.A. (2002) Lactate shuttles in nature. Biochem. Soc. Trans. 30, 258-264.
35. Poole,R.C. and Halestrap,A.P. (1993) Transport of lactate and other monocarboxylates across mammalian plasma membranes. Am. J. Physiol. 264, C761-C782.
36. Hermann,G.J., Thatcher,J.W., Mills,J.P., Hales,K.G., Fuller,M.T., Nunnari,J. and Shaw,J.M. (1998) Mitochondrial fusion in yeast requires the transmembrane GTPase Fzo1p. J. Cell Biol., 19, 359-373.
37. Semino-Mora,M.C., Leon-Monzon,M.E. and Dalakas,M.C. (1994) The effect of L-carnitine on the AZT-induced destruction of human myotubes. Part II: treatment with L-carnitine improves the AZT-induced changes and prevents further destruction. Lab. Invest., 71, 773-781.