Expression and purification of a recombinant Fip-fve protein from Flammulina velutipes in baculovirus-infected insect cells

Journal of Applied Microbiology

Volumn 104, Issue 5, 2008, Pages 1354-1362

  Wu, C M ^a   Wu, T Y ^b   Kao, S S ^a   Ko, J L ^c   Jinn, T R ^a  

^a TAIWAN AGRICULTURAL CHEMICALS AND TOXIC SUBSTANCES RESEARCH INSTITUTE   (Taiwan)

^b CHUNG YUAN CHRISTIAN UNIVERSITY   (Taiwan)

^c CHUNG SHAN MEDICAL UNIVERSITY   (Taiwan)

Author keywords

Allergy; Baculovirus insect cell expression system; Flammulina velutipes; Fungal immunomodulatory protein; Interleukin 2; Nickel affinity chromatography

Indexed keywords

CYTOLOGY; ESCHERICHIA COLI; GENE ENCODING; MASS SPECTROMETRY; NICKEL; PURIFICATION; RECOMBINANT PROTEINS; VIRUSES;

BACULOVIRUS; BACULOVIRUS/INSECT CELL EXPRESSION SYSTEM; CELL EXPRESSION; EXPRESSION SYSTEM; FLAMMULINA VELUTIPES; FUNGAL IMMUNOMODULATORY PROTEIN; IMMUNOMODULATORY; INSECT CELLS; INTERLEUKIN2 (IL2); NICKEL AFFINITIES; NICKEL-AFFINITY CHROMATOGRAPHY;

AFFINITY CHROMATOGRAPHY;

BOMBYXIN; GLYCOSYLATED PROTEIN; HISTONE; INTERLEUKIN 2; NICKEL; RECOMBINANT PROTEIN; RECOMBINANT PROTEIN FIP; RECOMBINANT PROTEIN FVE; SIGNAL PEPTIDE; UNCLASSIFIED DRUG;

CYTOLOGY; GENE EXPRESSION; MUSHROOM; PROTEIN; RECOMBINATION; VIRUS;

AFFINITY CHROMATOGRAPHY; ALLERGY; AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; BACULOVIRUS; CELL LINE; CHIMERA; CYTOKINE RELEASE; DRUG SCREENING; DRUG SYNTHESIS; ESCHERICHIA COLI; FLAMMULINA VELUTIPES; GENE; IMMUNOMODULATION; INSECT CELL; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN MODIFICATION; PROTEIN PURIFICATION; SPLEEN CELL; SUPERNATANT; VIRUS RECOMBINANT; WESTERN BLOTTING;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; FLAMMULINA VELUTIPES; HEXAPODA; MURINAE;

EID: 42149093297     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2007.03686.x     Document Type: Article

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