Expression of recombinant proteinase 3, the autoantigen in Wegener's granulomatosis, in insect cells

Journal of Immunological Methods

Volumn 264, Issue 1-2, 2002, Pages 195-205

  Van Der Geld, Y M ^a   Smook, M L F ^a   Huitema, M G ^a   Harmsen, M C ^a   Limburg, P C ^a   Kallenberg, C G M ^a  

^a UNIVERSITY MEDICAL CENTER GRONINGEN   (Netherlands)

Author keywords

ANCA; Baculovirus; Proteinase 3; Recombinant; Wegener's granulomatosis

Indexed keywords

AUTOANTIGEN; MELITTIN; MONOCLONAL ANTIBODY; MYELOBLASTIN; POLYHEDRIN; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ANTIGEN PURIFICATION; ARTICLE; BACULOVIRUS EXPRESSION SYSTEM; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DNA SEQUENCE; GENE AMPLIFICATION; GENE MAPPING; HONEYBEE; INSECT CELL; MIGRATION; MOLECULAR CLONING; NEUTROPHIL; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; RABBIT; VIRUS GENOME; WEGENER GRANULOMATOSIS; WILD TYPE;

ANIMALS; AUTOANTIGENS; BACULOVIRIDAE; BINDING SITES, ANTIBODY; BLOTTING, WESTERN; CELL LINE; CLONE CELLS; CLONING, MOLECULAR; ENZYME-LINKED IMMUNOSORBENT ASSAY; HISTIDINE; HUMANS; IMMUNE SERA; MYELOBLASTIN; PEPTIDE FRAGMENTS; PROTEIN PRECURSORS; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, PROTEIN; SERINE ENDOPEPTIDASES; SPODOPTERA; TIME FACTORS; WEGENER GRANULOMATOSIS;

APIS MELLIFERA; INSECTA; ORYCTOLAGUS CUNICULUS; UNIDENTIFIED BACULOVIRUS;

EID: 0036606658     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(02)00101-1     Document Type: Article

References (49)

1. Antineutrophil cytoplasmic autoantibodies in Wegener's granulomatosis recognize conformational epitope(s) on proteinase 3
2. Subcellular localization of the b-cytochrome component of the human neutrophil microbicidal oxidase: Translocation during activation
3. Cloning of cDNA for proteinase 3: A serine protease, antibiotic, and autoantigen from human neutrophils
4. Converting enzyme-independent release of tumor necrosis factor alpha and IL-1beta from a stimulated human monocytic cell line in the presence of activated neutrophils or purified proteinase 3
5. Association between active Wegener's granulomatosis and anticytoplasmic antibodies
6. Prevention of relapses in Wegener's granulomatosis by treatment based on antineutrophil cytoplasmic antibody titre
7. Autoantibodies against myeloid lysosomal enzymes in crescentic glomerulonephritis
8. Ultrastructural localization of proteinase 3, the target antigen of anti-cytoplasmic antibodies circulating in Wegener's granulomatosis
9. Activated neutrophils express proteinase 3 on their plasma membrane in vitro and in vivo
10. Transforming growth factor-beta (TGF-beta) expression and interaction with proteinase 3 (PR3) in anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis
11. Wegener's granulomatosis: Prospective clinical and therapeutic experience with 85 patients for 21 years
12. The crystal structure of PR3, a neutrophil serine proteinase antigen of Wegener's granulomatosis antibodies
13. Antibiotic proteins of human polymorphonuclear leukocytes
14. Characterization of the processing and granular targeting of human proteinase 3 after transfection to the rat RBL or the murine 32D leukemic cell lines
15. Wegener's granulomatosis autoantibodies identify a novel diisopropylfluorophosphate-binding protein in the lysosomes of normal human neutrophils
16. Development and standardization of solid phase assays for the detection of anti-neutrophil cytoplasmic antibodies (ANCA). A report on the second phase of an international cooperative study on the standardization of ANCA assays
17. Antineutrophil cytoplasmic antibodies (ANCA) directed against cathepsin G in ulcerative colitis, Crohn's disease and primary sclerosing cholangitis
18. Recombinant proteinase 3 (Wegener's antigen) expressed in Pichia pastoris is functionally active and is recognized by patient sera
19. A gas-liquid solid phase peptide and protein sequenator
20. Engineering N-glycosylation pathways in the baculovirus-insect cell system
21. Proteinase 3. A distinct human polymorphonuclear leukocyte proteinase that produces emphysema in hamsters
22. T lymphocyte responses to anti-neutrophil cytoplasmic autoantibody (ANCA) antigens are present in patients with ANCA-associated systemic vasculitis and persist during disease remission
23. The American College of Rheumatology 1990 criteria for the classification of Wegener's granulomatosis
24. Cleavage and inactivation of human C1 inhibitor by the human leukocyte proteinase, proteinase 3
25. Anti-neutrophil cytoplasm antibodies in Wegener's granulomatosis recognize an elastinolytic enzyme
26. Generation of active myeloid and lymphoid granule serine proteases requires processing by the granule thiol protease dipeptidyl peptidase I
27. Wegener's granulomatosis autoantigen is a novel neutrophil serine proteinase
28. Anticytoplasmic autoantibodies: Their immunodiagnostic value in Wegener granulomatosis
29. Interleukin-8 processing by neutrophil elastase, cathepsin G and proteinase-3
30. Characterization of proteinase-3 (PR-3), a neutrophil serine proteinase. Structural and functional properties
31. Biosynthesis and processing of proteinase 3 in U937 cells. Processing pathways are distinct from those of cathepsin G
32. In vitro processing of human tumor necrosis factor-alpha
33. Antineutrophil cytoplasmic antibodies reacting with the pro form of proteinase 3 and disease activity in patients with Wegener's granulomatosis and microscopic polyangiitis
34. Zymogen activation specificity and genomic structures of human neutrophil elastase and cathepsin G reveal a new branch of the chymotrypsinogen superfamily of serine proteinases
35. Wegener's granulomatosis: Anti-proteinase 3 antibodies are potent inductors of human endothelial cell signaling and leakage response
36. Primary structure of human neutrophil elastase
37. Recombinant human proteinase 3, the Wegener's autoantigen, expressed in HMC-1 cells is enzymatically active and recognized by c-ANCA
38. Human mast cells expressing recombinant proteinase 3 (PR3) as substrate for clinical testing for anti-neutrophil cytoplasmic antibodies (ANCA)
39. Structure, chromosomal assignment, and expression of the gene for proteinase-3. The Wegener's granulomatosis autoantigen
40. A proportion of proteinase 3 (PR3)-specific anti-neutrophil cytoplasmic antibodies (ANCA) only react with Pr3 after cleavage of its N-terminal activation dipeptide
41. Capture-ELISA based on recombinant PR3 is sensitive for PR3-ANCA testing and allows detection of PR3 and PR3-ANCA/PR3 immunecomplexes
42. Reactivities with recombinant human proteinase 3 of c-ANCA autoantibodies in patients with Wegener's Granulomatosis. (abstract)
43. Expression of the human autoantigen of Wegener's granulomatosis (PR3) in a baculovirus expression system
44. Characterization of monoclonal antibodies to proteinase 3 as candidate tools for epitope mapping of human anti-proteinase 3 autoantibodies
45. Recombinant proteinase 3 produced in different expression systems: Recognition by anti-PR3 antibodies
46. A technical note on the routinely performed ANCA detection
47. Autoantibodies against neutrophils and monocytes: Tool for diagnosis and marker of disease activity in Wegener's granulomatosis
48. Immobilised metal-ion affinity chromatography purification of histidine-tagged recombinant proteins: A wash step with a low concentration of EDTA
49. Characterization of a recombinant proteinase 3, the autoantigen in Wegener's granulomatosis and its reactivity with anti-neutrophil cytoplasmic autoantibodies