Identification of 19 polymorphic major outer membrane protein genes and their immunogenic peptides in Ehrlichia ewingii for use in a serodiagnostic assay

Clinical and Vaccine Immunology

Volumn 15, Issue 3, 2008, Pages 402-411

  Zhang, Chunbin ^a   Xiong, Qingming ^a   Kikuchi, Takane ^a   Rikihisa, Yasuko ^a  

^a OHIO STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GENE PRODUCT; OLIGOPEPTIDE; OUTER MEMBRANE PROTEIN; OUTER MEMBRANE PROTEIN 1; PEPTIDE DERIVATIVE; SYNTHETIC PEPTIDE; BACTERIAL ANTIGEN; BACTERIUM ANTIBODY; DIAGNOSTIC AGENT; PEPTIDE;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIGENICITY; ARTICLE; BACTERIAL GENE; BACTERIAL GENOME; CONTROLLED STUDY; DOG; EHRLICHIA; EHRLICHIA CHAFFEENSIS; EHRLICHIA EWINGII; EHRLICHIA RUMINANTIUM; EHRLICHIOSIS; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE IDENTIFICATION; IMMUNOGENICITY; ISOELECTRIC POINT; MOLECULAR WEIGHT; MULTIGENE FAMILY; NONHUMAN; NUCLEOTIDE SEQUENCE; OMP 1 GENE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; SECA GENE; SEQUENCE ANALYSIS; SERODIAGNOSIS; TRI GENE; ANIMAL; ANIMAL DISEASE; BLOOD; CHEMISTRY; DNA SEQUENCE; DOG DISEASE; EVALUATION; GENETIC POLYMORPHISM; GENETICS; HUMAN; IMMUNOLOGY; METABOLISM; MICROBIOLOGY; MOLECULAR GENETICS; PHYLOGENY; SYNTHESIS;

ANIMALS; ANTIBODIES, BACTERIAL; ANTIGENS, BACTERIAL; BACTERIAL OUTER MEMBRANE PROTEINS; DOG DISEASES; DOGS; EHRLICHIA; EHRLICHIOSIS; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; MOLECULAR SEQUENCE DATA; PEPTIDES; PHYLOGENY; POLYMORPHISM, GENETIC; SEQUENCE ANALYSIS, DNA;

EID: 42049107798     PISSN: 15566811     EISSN: 1556679X     Source Type: Journal    
DOI: 10.1128/CVI.00366-07     Document Type: Article

References (53)

1. Anderson, B. E., C. E. Greene, D. C. Jones, and J. E. Dawson. 1992. Ehrlichia ewingii sp. nov., the etiologic agent of canine granulocytic ehrlichiosis. Int. J. Syst. Bacteriol. 42:299-302.
2. Anziani, O. S., S. A. Ewing, and R. W. Barker. 1990. Experimental transmission of a granulocytic form of the tribe Ehrlichieae by Dermacentor variabilis and Amblyomma americanum to dogs. Am. J. Vet. Res. 51:929-931.
3. Bagos, P. G., T. D. Liakopoulos, I. C. Spyropoulos, and S. J. Hamodrakas. 2004. PRED-TMBB: a web server for predicting the topology of beta-barrel outer membrane proteins. Nucleic Acids Res. 32:W400-W404.
4. Bakken, J. S., and J. S. Dumler. 2006. Clinical diagnosis and treatment of human granulocytotropic anaplasmosis. Ann. N. Y. Acad. Sci. 1078:236-247.
5. Bell-Sakyi, L., E. B. Koney, O. Dogbey, K. J. Sumption, A. R. Walker, A. Bath, and F. Jongejan. 2003. Detection by two enzyme-linked immunosorbent assays of antibodies to Ehrlichia ruminantium in field sera collected from sheep and cattle in Ghana. Clin. Diagn. Lab. Immunol. 10:917-925.
6. Buller, R. S., M. Arens, S. P. Hmiel, C. D. Paddock, J. W. Sumner, Y. Rikhisa, A. Unver, M. Gaudreault-Keener, F. A. Manian, A. M. Liddell, N. Schmulewitz, and G. A. Storch. 1999. Ehrlichia ewingii, a newly recognized agent of human ehrlichiosis. N. Engl. J. Med. 341:148-155.
7. Carver, T. J., K. M. Rutherford, M. Berriman, M. Rajandream, B. G. Barrell and Julian Parkhill. 2005. ACT: the Artemis comparison tool. Bioinformatics 21:3422-3423.
8. Dawson, J. E., K. L. Biggie, C. K. Warner, K. Cookson, S. Jenkins, J. F. Levine, and J. G. Olson. 1996. Polymerase chain reaction evidence of Ehrlichia chaffeensis, an etiologic agent of human ehrlichiosis, in dogs from southeast Virginia. Am. J. Vet. Res. 57:1175-1179.
9. Demma, L. J., R. C. Holman, J. H. McQuiston, J. W. Krebs, and D. L. Swerdlow. 2005. Epidemiology of human ehrlichiosis and anaplasmosis in the United States, 2001-2002. Am. J. Trop. Med. Hyg. 73:400-409.
10. Ewing, S. A., J. E. Dawson, A. A. Kocan, R. W. Barker, C. K. Warner, R. J. Panciera, J. C. Fox, K. M. Kocan, and E. F. Blouin. 1995. Experimental transmission of Ehrlichia chaffeensis (Rickettsiales: Ehrlichieae) among white-tailed deer by Amblyomma americanum (Acari: Ixodidae). J. Med. Entomol. 32:368-374.
11. Ewing, S. A., W. R. Roberson, R. G. Buckner, and C. S. Hayat. 1971. A new strain of Ehrlichia canis. J. Am. Vet. Med. Assoc. 159:1771-1774.
12. Faburay, B., S. Munstermann, D. Geysen, L. Bell-Sakyi, A. Ceesay, C. Bodaan, and F. Jongejan. 2005. Point seroprevalence survey of Ehrlichia ruminantium infection in small ruminants in The Gambia. Clin. Diagn. Lab. Immunol. 12:508-512.
13. Felsenstein, J. 1989. PHYLIP - phylogeny inference package (version 3.2). Cladistics 5:164-166.
14. Ge, Y., and Y. Rikihisa. 2007. Surface-exposed proteins of Ehrlichia chaffeensis. Infect. Immun. 75:3833-3841.
15. Geysen, H. M. 1990. Molecular technology: peptide epitope mapping and the pin technology. Southeast Asian J. Trop. Med. Public Health 21:523-533.
16. Goldman, E. E., E. B. Breitschwerdt, C. B. Grindem, B. C. Hegarty, J. J. Walls, and J. S. Dumler. 1998. Granulocytic ehrlichiosis in dogs from North Carolina and Virginia. J. Vet. Intern. Med. 12:61-70.
17. Gusa, A. A., R. S. Buller, G. A. Storch, M. M. Huycke, L. J. Machado, L. N. Slater, S. L. Stockham, and R. F. Massung. 2001. Identification of a p28 gene in Ehrlichia ewingii: evaluation of gene for use as a target for a speciesspecific PCR diagnostic assay. J. Clin. Microbiol. 39:3871-3876.
18. Ijdo, J. W., W. Sun, Y. Zhang, L. A. Magnarelli, and E. Fikrig. 1998. Cloning of the gene encoding the 44-kilodalton antigen of the agent of human granulocytic ehrlichiosis and characterization of the humoral response. Infect. Immun. 66:3264-3269.
19. Labruna, M. B., J. W. McBride, L. M. Camargo, D. M. Aguiar, M. J. Yabsley, W. R. Davidson, E. Y. Stromdahl, P. C. Williamson, R. W. Stich, S. W. Long, E. P. Camargo, and D. H. Walker. 2007. A preliminary investigation of Ehrlichia species in ticks, humans, dogs, and capybaras from Brazil. Vet. Parasitol. 143:189-195.
20. Li, J. S., F. Chu, A. Reilly, and G. M. Winslow. 2002. Antibodies highly effective in SCID mice during infection by the intracellular bacterium Ehrlichia chaffeensis are of picomolar affinity and exhibit preferential epitope and isotype utilization. J. Immunol. 169:1419-1425.
21. Liddell, A. M., S. L. Stockham, M. A. Scott, J. W. Sumner, C. D. Paddock, M. Gaudreault-Keener, M. Q. Arens, and G. A. Storch. 2003. Predominance of Ehrlichia ewingii in Missouri dogs. J. Clin. Microbiol. 41:4617-4622.
22. Lodes, M. J., R. Mohamath, L. D. Reynolds, P. McNeill, C. P. Kolbert, E. S. Bruinsma, D. R. Benson, E. Hofmeister, S. G. Reed, R. L. Houghton, and D. H. Persing. 2001. Serodiagnosis of human granulocytic ehrlichiosis by using novel combinations of immunoreactive recombinant proteins. J. Clin. Microbiol. 39:2466-2476.
23. Magnarelli, L. A., J. W. Ijdo, A. E. Van Andel, C. Wu, and E. Fikrig. 2001. Evaluation of a polyvalent enzyme-linked immunosorbent assay incorporating a recombinant p44 antigen for diagnosis of granulocytic ehrlichiosis in dogs and horses. Am. J. Vet. Res. 62:29-32.
24. McBride, J. W., R. E. Corstvet, E. B. Breitschwerdt, and D. H. Walker. 2001. Immunodiagnosis of Ehrlichia canis infection with recombinant proteins. J. Clin. Microbiol. 39:315-322.
25. McBride, J. W., X. J. Yu, and D. H. Walker. 2000. A conserved, transcriptionally active p28 multigene locus of Ehrlichia canis. Gene 254:245-252.
26. Murphy, G. L., S. A. Ewing, L. C. Whitworth, J. C. Fox, and A. A. Kocan. 1998. A molecular and serologic survey of Ehrlichia canis, E. chaffeensis, and E. ewingii in dogs and ticks from Oklahoma. Vet. Parasitol. 79:325-339.
27. Ndip, L. M., R. N. Ndip, S. N. Esemu, V. L. Dickmu, E. B. Fokam, D. H. Walker, and J. W. McBride. 2005. Ehrlichial infection in Cameroonian canines by Ehrlichia canis and Ehrlichia ewingii. Vet. Microbiol. 111:59-66.
28. Ohashi, N., Y. Rikihisa, and A. Unver. 2001. Analysis of transcriptionally active gene clusters of major outer membrane protein multigene family in Ehrlichia canis and E. chaffeensis. Infect. Immun. 69:2083-2091.
29. Ohashi, N., A. Unver, N. Zhi, and Y. Rikihisa. 1998. Cloning and characterization of multigenes encoding the immunodominant 30-kilodalton major outer membrane proteins of Ehrlichia canis and application of the recombinant protein for serodiagnosis. J. Clin. Microbiol. 36:2671-2680.
30. Ohashi, N., N. Zhi, Y. Zhang, and Y. Rikihisa. 1998. Immunodominant major outer membrane proteins of Ehrlichia chaffeensis are encoded by a polymorphic multigene family. Infect. Immun. 66:132-139.
31. Paddock, C. D., and J. E. Childs. 2003. Ehrlichia chaffeensis: a prototypical emerging pathogen. Clin. Microbiol. Rev. 16:37-64.
32. Peter, T. F., C. J. O'Callaghan, G. F. Medley, B. D. Perry, S. M. Semu, and S. M. Maha. 2001. Population-based evaluation of the Ehrlichia ruminantium MAP 1B indirect ELISA. Exp. Appl. Acarol. 25:881-897.
33. Reddy, G. R., C. R. Sulsona, A. F. Barbet, S. M. Mahan, M. J. Burridge, and A. R. Alleman. 1998. Molecular characterization of a 28 kDa surface antigen gene family of the tribe Ehrlichiae. Biochem. Biophys. Res. Commun. 247:636-643.
34. Rikihisa, Y., S. A. Ewing, and J. C. Fox. 1994. Western immunoblot analysis of Ehrlichia chaffeensis, E. canis, or E. ewingii infections in dogs and humans. J. Clin. Microbiol. 32:2107-2112.
35. Rikihisa, Y., S. A. Ewing, J. C. Fox, A. G. Siregar, F. H. Pasaribu, and M. B. Malole. 1992. Analyses of Ehrlichia canis and a canine granulocytic Ehrlichia infection. J. Clin. Microbiol. 30:143-148.
36. Rikihisa, Y., C. Zhang, and B. M. Christensen. 2003. Molecular characterization of Aegyptianella pullorum (Rickettsiales, Anaplasmataceae). J. Clin. Microbiol. 41:5294-5297.
37. Roux, K. H., and K. H. Hecker. 1997. One-step optimization using touch-down and stepdown PCR. Methods Mol. Biol. 67:39-45.
38. Rutherford, J., J. Parkhill, J. Crook, T. Horsnell, P. Rice, M. Rajandream, and B. Barrell. 2000. Artemis: sequence visualization and annotation. Bioinformatics 16:944-945.
39. Sumner, J. W., G. A. Storch, R. S. Buller, A. M. Liddell, S. L. Stockham, Y. Rikihisa, S. Messenger, and C. D. Paddock. 2000. PCR amplification and phylogenetic analysis of groESL operon sequences from Ehrlichia ewingii and Ehrlichia muris. J. Clin. Microbiol. 38:2746-2749.
40. Tajima, T., N. Zhi, Q. Lin, Y. Rikihisa, H. W. Horowitz, J. Ralfalli, G. P. Wormser, and K. E. Hechemy. 2000. Comparison of two recombinant major outer membrane proteins of the human granulocytic ehrlichiosis agent for use in an enzyme-linked immunosorbent assay. Clin. Diagn. Lab. Immunol. 7:652-657.
41. Unver, A., S. Felek, C. D. Paddock, N. Zhi, H. W. Horowitz, G. P. Wormser, L. C. Cullman, and Y. Rikihisa. 2001. Western blot analysis of sera reactive to human monocytic ehrlichiosis and human granulocytic ehrlichiosis agents. J. Clin. Microbiol. 39:3982-3986.
42. Unver, A., N. Ohashi, T. Tajima, R. W. Stich, D. Grover, and Y. Rikihisa. 2001. Transcriptional analysis of p30 major outer membrane multigene family of Ehrlichia canis in dogs, ticks, and cell culture at different temperatures. Infect. Immun. 69:6172-6178.
43. Unver, A., Y. Rikihisa, N. Ohashi, L. C. Cullman, R. Buller, and G. A. Storch. 1999. Western and dot blotting analyses of Ehrlichia chaffeensis indirect fluorescent-antibody assay-positive and -negative human sera by using native and recombinant E. chaffeensis and E. canis antigens. J. Clin. Microbiol. 37:3888-3895.
44. Unver, A., Y. Rikihisa, R. W. Stich, N. Ohashi, and S. Felek. 2002. The omp-1 major outer membrane multigene family of Ehrlichia chaffeensis is differentially expressed in canine and tick hosts. Infect. Immun. 70:4701-4704.
45. van Heerden, H., N. E. Collins, K. A. Brayton, C. Rademeyer, and B. A. Allsopp. 2004. Characterization of a major outer membrane protein multigene family in Ehrlichia ruminantium. Gene 330:159-168.
46. van Vliet, A. H., F. Jongejan, M. van Kleef, and B. A. van der Zeijst. 1994. Molecular cloning, sequence analysis, and expression of the gene encoding the immunodominant 32-kilodalton protein of Cowdria ruminantium. Infect. Immun. 62:1451-1456.
47. Xiong, Q., W. Bao, Y. Ge, and Y. Rikihisa. Ehrlichia ewingii infection delays spontaneous neutrophil apoptosis through stabilization of mitochondria. J. Infect. Dis., in press.
48. Yabsley, M. J., A. S. Varela, C. M. Tate, V. G. Dugan, D. E. Stallknecht, S. E. Little, and W. R. Davidson. 2002. Ehrlichia ewingii infection in white-tailed deer (Odocoileus virginianus). Emerg. Infect. Dis. 8:668-671.
49. Yu, X., J. W. McBride, X. Zhang, and D. H. Walker. 2000. Characterization of the complete transcriptionally active Ehrlichia chaffeensis 28 kDa outer membrane protein multigene family. Gene 248:59-68.
50. Yu, X. J., P. Crocquet-Valdes, L. C. Cullman, and D. H. Walker. 1996. The recombinant 120-kilodalton protein of Ehrlichia chaffeensis, a potential diagnostic tool. J. Clin. Microbiol. 34:2853-2855.
51. Yu, X. J., P. A. Crocquet-Valdes, L. C. Cullman, V. L. Popov, and D. H. Walker. 1999. Comparison of Ehrlichia chaffeensis recombinant proteins for serologic diagnosis of human monocytotropic ehrlichiosis. J. Clin. Microbiol. 37:2568-2575.
52. Zhang, J. Z., H. Guo, G. M. Winslow, and X. J. Yu. 2004. Expression of members of the 28-kilodalton major outer membrane protein family of Ehrlichia chaffeensis during persistent infection. Infect. Immun. 72:4336-4343.
53. Zhi, N., N. Ohashi, Y. Rikihisa, H. W. Horowitz, G. P. Wormser, and K. Hechemy. 1998. Cloning and expression of the 44-kilodalton major outer membrane protein gene of the human granulocytic ehrlichiosis agent and application of the recombinant protein to serodiagnosis. J. Clin. Microbiol. 36:1666-1673.