Genetic profiling of Trypanosoma cruzi directly in infected tissues using nested PCR of polymorphic microsatellites

International Journal for Parasitology

Volumn 38, Issue 7, 2008, Pages 839-850

  Valadares, Helder Magno Silva ^a   Pimenta, Juliana Ramos ^a   de Freitas, Jorge Marcelo ^a   Duffy, Tomás ^b   Bartholomeu, Daniella C ^a   de Paula Oliveira, Riva ^c   Chiari, Egler ^a   Moreira, Maria da Consolação Vieira ^a   Filho, Geraldo Brasileiro ^a   Schijman, Alejandro Gabriel ^b   Franco, Glória Regina ^a   Machado, Carlos Renato ^a   Pena, Sérgio Danilo Junho ^a   Macedo, Andréa Mara ^a  

^a FEDERAL UNIVERSITY OF MINAS GERAIS   (Brazil)

^b INSTITUTO DE INVESTIGACIONES EN INGENIERÍA GENÉTICA Y BIOLOGÍA MOLECULAR   (Argentina)

^c JOSLIN DIABETES CENTER   (United States)

Author keywords

Chagas disease; Full nested PCR; Genome project; Polymorphic microsatellites; Trypanosoma cruzi

Indexed keywords

ADENINE; CONTIG; CYTOSINE; GUANINE; NUCLEOTIDE; PRIMER DNA; TETRANUCLEOTIDE; THYMINE; TRINUCLEOTIDE; UNCLASSIFIED DRUG;

CHAGAS DISEASE; CHROMOSOME; GENETIC ANALYSIS; GENETIC MARKER; GENETIC VARIATION; GENOME; POLYMERASE CHAIN REACTION; PROTEIN; PROTOZOAN; SOFTWARE;

ANIMAL TISSUE; ARTICLE; CHAGAS DISEASE; COMPUTER PROGRAM; DISEASE COURSE; DNA FLANKING REGION; GENE LOCUS; GENE SEQUENCE; GENETIC VARIABILITY; GENOME; HUMAN; HUMAN TISSUE; MALE; MICROSATELLITE INSTABILITY; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; STRAIN IDENTIFICATION; TANDEM REPEAT; TRYPANOSOMA CRUZI;

ANIMALS; CHAGAS DISEASE; CHROMOSOME MAPPING; CHRONIC DISEASE; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FEMALE; GENE FREQUENCY; GENES, PROTOZOAN; HEART; HUMANS; MALE; MICE; MICE, INBRED BALB C; MICROSATELLITE REPEATS; PARASITEMIA; POLYMERASE CHAIN REACTION; POLYMORPHISM, GENETIC; PREGNANCY; RECTUM; SEQUENCE ALIGNMENT; SKIN; TRYPANOSOMA CRUZI;

TRYPANOSOMA CRUZI;

EID: 41949140657     PISSN: 00207519     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ijpara.2007.10.017     Document Type: Article

References (42)

1. Andersson B., Aslund L., Tammi M., Tran A.N., Hoheisel J.D., and Pettersson U. Complete sequence of a 93.4-kb contig from chromosome 3 of Trypanosoma cruzi containing a strand-switch region. Genome Res. 8 (1998) 809-816
2. Andrade L.O., Machado C.R., Chiari E., Pena S.D., and Macedo A.M. Differential tissue distribution of diverse clones of Trypanosoma cruzi in infected mice. Mol. Biochem. Parasitol. 100 (1999) 163-172
3. Andrade S.G. Morphological and behavioral characterization of Trypanosoma cruzi strains. Rev. Soc. Bras. Med. Trop. 18 (1985) 39-46
4. Añez N., Crisante G., da Silva F.M., Rojas A., Carrasco H., Umezawa E.S., Stolf A.M., Ramírez J.L., and Teixeira M.M. Predominance of lineage I among Trypanosoma cruzi isolates from Venezuelan patients with different clinical profiles of acute Chagas' disease. Trop. Med. Int. Health 9 12 (2004) 1319-1326
5. Ayala F.J. Trypanosoma and Leishmania have clonal population structures of epidemiological significance. Biol. Res. 26 (1993) 47-63
6. Benson G. Tandem Repeats Finder: a program to analyze DNA sequences. Nucleic Acids Res. 27 (1999) 573-580
7. Branche C., Ochaya S., Aslund L., and Andersson B. Comparative karyotyping as a tool for genome structure analysis of Trypanosoma cruzi. Mol. Biochem. Parasitol. 147 (2006) 30-38
8. Brisse S., Barnabé C., and Tibayrenc M. Identification of six Trypanosoma cruzi phylogenetic lineages by random amplified polymorphic DNA and multilocus enzyme electroforesis. Int. J. Parasitol. 30 (2000) 34-44
9. Brisse S., Verhoef J., and Tibayrenc M. Characterization of large and small subunit rRNA and mini-exon genes further supports the distinction of six Trypanosoma cruzi lineages. Int. J. Parasitol. 31 (2001) 1218-1226
10. Burgos J.M., Begher S., Freitas J.M., Bisio M., Duffy T., Altcheh J., Teijeiro R., Lopez Alcoba H., Deccarlini F., Freilij H., Levin M.J., Levalle J., Macedo A.M., and Schijman A.G. Molecular diagnosis and typing of Trypanosoma cruzi populations and lineages in cerebral Chagas disease in a patient with AIDS. Am. J. Trop. Med. Hyg. 73 (2005) 1016-1018
11. Burgos J.M., Altcheh J., Bisio M., Duffy T., Valadares H.M.S., Piccinali R., Freitas J.M., Levin M.J., Macedo A.M., Freilij H., and Schijman A.G. Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease. Int. J. Parasitol. 37 12 (2007) 1319-1327
12. Coura J.R., Junqueira A.C., Fernandes O., Valente A.S., and Miles M.A. Emerging Chagas disease in Amazonian Brazil. Trends Parasitol. 18 4 (2002) 171-176
13. Di Noia J.M., Buscaglia C.A., De Marchi C.R., Almeida I.C., and Frasch A.C. A Trypanosoma cruzi small surface molecule provides the first immunological evidence that Chagas' disease is due to a single parasite lineage. J. Exp. Med. 185 (2002) 401-413
14. El-Sayed N.M., Myler P.J., Bartholomeu D.C., Nilsson D., Aggarwal G., Tran A.N., Ghedin E., Worthey E.A., Delcher A.L., Blandin G., Westenberger S.J., Caler E., Cerqueira G.C., Branche C., Haas B., Anupama A., Arner E., Aslund L., Attipoe P., Bontempi E., Bringaud F., Burton P., Cadag E., Campbell D.A., Carrington M., Crabtree J., Darban H., da Silveira J.F., de Jong P., Edwards K., Englund P.T., Fazelina G., Feldblyum T., Ferella M., Frasch A.C., Gull K., Horn D., Hou L., Huang Y., Kindlund E., Klingbeil M., Kluge S., Koo H., Lacerda D., Levin M.J., Lorenzi H., Louie T., Machado C.R., McCulloch R., McKenna A., Mizuno Y., Mottram J.C., Nelson S., Ochaya S., Osoegawa K., Pai G., Parsons M., Pentony M., Pettersson U., Pop M., Ramirez J.L., Rinta J., Robertson L., Salzberg S.L., Sanchez D.O., Seyler A., Sharma R., Shetty J., Simpson A.J., Sisk E., Tammi M.T., Tarleton R., Teixeira S., Van Aken S., Vogt C., Ward P.N., Wickstead B., Wortman J., White O., Fraser C.M., Stuart K.D., and Andersson B. The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease. Science 309 (2005) 409-415
15. Freitas J.M., Augusto-Pinto L., Pimenta J.R., Bastos-Rodrigues L., Goncalves V.F., Teixeira S.M., Chiari E., Junqueira A.C., Fernandes O., Macedo A.M., Machado C.R., and Pena S.D. Ancestral genomes, Sex and the Population Structure of Trypanosoma cruzi. PLoS Pathogens 2 (2006) 226-235
16. Freitas J.M., Lages-Silva E., Crema E., Pena S.D.J., and Macedo A.M. Real time PCR strategy for the identification of major lineages of Trypanosoma cruzi directly in chronically infected human tissues. Int. J. Parasitol. 35 (2005) 411-417
17. Gaunt M.W., Yeo M., Frame I.A., Stothard J.R., Carrasco H.J., Taylor M.C., Mena S.S., Veazey P., Miles G.A., Acosta N., de Arias A.R., and Miles M.A. Mechanism of genetic exchange in American trypanosomes. Nature 241 (2003) 936-939
18. Higgins D., Thompson J., Gibson T., Thompson J.D., Higgins D.G., and Gibson T.J. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22 (1994) 4673-4680
19. Hecker K.H., and Roux K.H. High and low annealing temperatures increase both specificity and yield in touchdown and step-down PCR. Biotechniques 20 (1996) 478-485
20. Macedo A.M., and Pena S.D.J. Genetic variability of Trypanosoma cruzi: implication for the pathogenesis of Chagas' disease. Parasitol. Today 14 (1998) 119-123
21. Macedo A.M., Martins M.S., Chiari E., and Pena S.D. DNA fingerprinting of Trypanosoma cruzi: a new tool for characterization of strain and clones. Mol. Biochem. Parasitol. 55 (1992) 147-154
22. Macedo A.M., Pimenta J.R., Aguiar R.S., Melo A.R., Chiari E., Zingales B., Pena S.D.J., and Oliveira R.P. Usefulness of microsatellite typing in population genetic studies of Trypanosoma cruzi. Mem. Inst. Oswaldo Cruz 96 (2001) 407-413
23. Macedo A.M., Machado C.R., Oliveira R.P., and Pena S.D.J. Trypanosoma cruzi: genetic structure of populations and relevance of genetic variability to the pathogenesis of Chagas disease. Mem. Inst. Oswaldo Cruz 99 (2004) 1-12
24. Machado C.A., and Ayala F.J. Nucleotide sequences provide evidence of genetic exchange among distantly related lineages of Trypanosoma cruzi. Proc. Natl. Acad. Sci. USA 98 (2001) 7396-7401
25. Melo R.C., and Brener Z. Tissue tropism of different Trypanosoma cruzi strains. J. Parasitol. 64 (1978) 475-482
26. Miles M.A., Souza A., Povoa M., Shaw J.J., Lainson R., and Toye P.J. Isozymic heterogeneity of Trypanosoma cruzi in the first autochthonous patients with Chagas' disease in Amazoniam Brazil. Nature 272 (1978) 819-821
27. Momen H. Taxonomy of Trypanosoma cruzi: a commentary on characterization and nomenclature. Mem. Inst. Oswaldo Cruz 94 (1999) 181-184
28. Morel C., Chiari E., Camargo E.P., Mattei D.M., Romanha A.J., and Simpson L. Strain and clones of Trypanosoma cruzi can be characterized by patterns of restriction endonuclease products of kinetoplast DNA minicircles. Proc. Natl. Acad. Sci. USA 77 (1980) 6810-6814
29. Oliveira R.P., Broude N.E., Macedo A.M., Cantor C.R., Smith C.L., and Pena S.D. Probing the genetic population structure of Trypanosoma cruzi with polymorphic microsatellites. Proc. Natl. Acad. Sci. USA 95 (1998) 3376-3780
30. Oliveira R.P., Melo A.I.R., Macedo A.M., Chiari E., and Pena S.D.J. The population structure of Trypanosoma cruzi: expanded analysis of 54 strains using eight polymorphic CA-repeat microsatellites. Mem. Inst. Oswaldo Cruz 94 (1999) 65-70
31. Romanha A.J., Pereira da Silva A.A., Chiari E., and Kilgour V. Isoenzyme patterns of cultured Trypanosoma cruzi: changes after prolonged subculture. Comp. Biochem. Physiol. 62B (1979) 139-142
32. Rychlick, W., 1992. OLIGO version 4.0. National Biosciences, Plymouth, Minnesota.
33. Schijman A., Vigliano C., Burgos J.M., Favaloro R., Perrone S., Laguens R., and Levin M.J. Early diagnosis of recurrence of T. cruzi infection by polymerase chain reaction after heart transplantation of a chronic Chagas' heart disease patient. J. Heart Lung Transplant. 19 (2000) 1114-1117
34. Souto R.P., Fernandes O., Macedo A.M., Campbell D.A., and Zingales B. DNA markers define two major phylogenetic lineages of Trypanosoma cruzi. Mol. Biochem. Parasitol. 83 (1996) 141-152
35. Teixeira M.M.G., daSilva F.M., Marcili A., Umezawa E.S., Shikanai-Yasuda M.A., Cunha-Neto E., Kalil J., Stolf N., and Stolf A.M. Trypanosoma cruzi lineage I in endomyocardail biopsy from a north-eastern Brazilian patient at end-stage chronic chagasic cardiomyopathy. Trop. Med. Int. Health 11 3 (2006) 294-298
36. Tibayrenc M., Ward P., Moya A., and Ayala F.J. Natural populations of Trypanosoma cruzi, the agent of Chagas' Disease, have a complex multiclonal structure. Proc. Natl. Acad. Sci. USA 83 (1986) 1335-1339
37. Tibayrenc M. Population genetics of parasitic protozoa and other microorganisms. Adv. Parasitol. 36 (1995) 47-115
38. Vago A.R., Andrade L.O., Leite A.A., d'Avila Reis D., Macedo A.M., Adad S.J., Tostes Jr. S., Moreira M.C., Filho G.B., and Pena S.D.J. Genetic characterization of Trypanosoma cruzi directly from tissues of patients with chronic Chagas disease: differential distribution of genetic types into diverse organs. Am. J. Pathol. 156 (2000) 1805-1809
39. Veas F., Breniere S.F., Cuny G., Brengues C., Solari A., and Tibayrenc M. General procedure to construct highly specific kDNA probes for clones of Trypanosoma cruzi for sensitive detection by polymerase chain reaction. Cell Mol. Biol. 37 1 (1991) 73-84
40. Virreira M., Serrano G., Maldonado L., and Svoboda M. Trypanosoma cruzi: typing of genotype (sub) lineages in megacolon samples from bolivian patients. Acta Trop. 100 3 (2006) 252-255
41. Zingales B., Souto R.P., Mangia R.H., Lisboa C.V., Campbell D.A., Coura J.R., Jansen A., and Fernandes O. Molecular epidemiology of American trypanosomiasis in Brazil based on dimorphisms of rRNA and mini-exon gene sequences. Int. J. Parasitol. 28 (1998) 105-112
42. Zingales B., Stolf B.S., Souto R.P., Fernandes O., and Briones M.R. Epidemiology, biochemistry and evolution of Trypanosoma cruzi lineages based on ribosomal RNA sequences. Mem. Inst. Oswaldo Cruz 94 (1999) 159-164