High and low annealing temperatures increase both specificity and yield in touchdown and stepdown PCR

BioTechniques

Volumn 20, Issue 3, 1996, Pages 478-485

  Hecker, Karl H ^a   Roux, Kenneth H ^a  

^a FLORIDA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA;

ANIMAL TISSUE; ARTICLE; GENE AMPLIFICATION; INTERMETHOD COMPARISON; LIVER; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; RABBIT; TEMPERATURE SENSITIVITY;

EID: 0029923481     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/19962003478     Document Type: Article

References (11)

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2. D'Aquila, R.T., L.J. Bechtel, J.A. Videler, J.J. Eron, P. Gorczyca and J. C. Kaplan. 1991 Maximizing sensitivity and specificity of PCR by preamplification heating. Nucleic Acids Res. 29:3149.
3. Don, R.H., P.T. Cox, B.J. Wainwright, K. Baker and J.S. Mattick. 1991. 'Touchdown' PCR to circumvent spurious priming during gene amplification. Nucleic Acids Res. 19:4008.
4. Erlich, H.A., D. Gelfand and J.J. Sninsky. 1991. Recent advances in the polymerase chain reaction. Science 252.1643-1651.
5. Innis, M.A. and D.H. Gelfand. 1990. Optimization in PCRs, p. 3-12. In M.A. Innis, D.H. Gelfand, J.J. Sninsky and T.J. White (Eds.), PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego.
6. Mullis, K.B. 1991. The polymerase chain reaction in an anemic mode: how to avoid cold oligodeoxyribonuclear fusion. PCR Methods Appl. 1:1-4.
7. Newton, C.R. and A. Graham. 1994. PCR. Bios Scientific Publishers, Oxford.
8. Roux, K.H. 1994. Using mismatched primer-template pairs in touchdown PCR. BioTechniques 16:812-814.
9. Roux, K.H. 1995. Optimization and troubleshooting in PCR. PCR Methods Appl. 4: S185-S194.
10. Rychlik, W. 1994. New algorithm for determining primer efficiency in PCR and sequencing. J. NIH Res. 6:78.
11. Rychlik, W. and R.E. Rhoads. 1989. A computer program for choosing optimal oligonucleotides for filter hybridization, sequencing and in vitro amplification of DNA. Nucleic Acids Res. 17:8543-8551.