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Phospholipid flippases
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This brief mini-review provides a summary of all classes of lipid transporters, •
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Daleke DL. Phospholipid flippases. J Biol Chem 2007; 282:821-825. • This brief mini-review provides a summary of all classes of lipid transporters.
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Lenoir G, Williamson P, Holthuis JC. On the origin of lipid asymmetry: the flip side of ion transport. Curr Opin Chem Biol 2007; 11:654-661. This is a good review of the current status of knowledge of the P4-ATPase family and offers a theory of how P4-ATPases might transport lipids.
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Lenoir G, Williamson P, Holthuis JC. On the origin of lipid asymmetry: the flip side of ion transport. Curr Opin Chem Biol 2007; 11:654-661. This is a good review of the current status of knowledge of the P4-ATPase family and offers a theory of how P4-ATPases might transport lipids.
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ABC lipid transporters: Extruders, flippases, or flopless activators?
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Smriti, Nemergut EC, Daleke DL. ATP-dependent transport of phosphatidylserine analogues in human erythrocytes. Biochemistry 2007; 46:2249-2259. These studies report that with the exception of phosphatidylcholine, lipids of a variety of headgroup structures move across the membrane in a vanadate-sensitive and NEM-sensitive manner, criteria that have been used to characterize the phosphatidylserine-selective flippase. These results question current assumptions about the number and specificity of erythrocyte flippases.
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Smriti, Nemergut EC, Daleke DL. ATP-dependent transport of phosphatidylserine analogues in human erythrocytes. Biochemistry 2007; 46:2249-2259. These studies report that with the exception of phosphatidylcholine, lipids of a variety of headgroup structures move across the membrane in a vanadate-sensitive and NEM-sensitive manner, criteria that have been used to characterize the phosphatidylserine-selective flippase. These results question current assumptions about the number and specificity of erythrocyte flippases.
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Phospholipid inhibitors of human erythrocyte phosphatidylserine flippase activity [abstract]
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Smriti S, Nemergut E, Daleke D. Phospholipid inhibitors of human erythrocyte phosphatidylserine flippase activity [abstract]. Biophys J 2007; 92:174a.
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Daleke DL, Lyles JV. Identification and purification of aminophospholipid flippases. Biochim Biophys Acta 2000; 1486:108-127.
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Paulusma CC, Folmer DE, Ho-Mok KS, et al. ATP8B1 requires an accessory protein for endoplasmic reticulum exit and plasma membrane lipid flippase activity. Hepatology 2008; 47:268-278. Although this report is of studies in hepatocytes, it is the first to demonstrate the role for a P4-ATPase in phosphatidylserine transport in mammalian cells and to illustrate the requirement for CDC50 partner protein for proper intracellular trafficking.
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Paulusma CC, Folmer DE, Ho-Mok KS, et al. ATP8B1 requires an accessory protein for endoplasmic reticulum exit and plasma membrane lipid flippase activity. Hepatology 2008; 47:268-278. Although this report is of studies in hepatocytes, it is the first to demonstrate the role for a P4-ATPase in phosphatidylserine transport in mammalian cells and to illustrate the requirement for CDC50 partner protein for proper intracellular trafficking.
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This recent review summarizes the current state of information about phospholipid scramblases
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Sahu SK, Gummadi SN, Manoj N, Aradhyam GK. Phospholipid scramblases: an overview. Arch Biochem Biophys 2007; 462:103-114. This recent review summarizes the current state of information about phospholipid scramblases.
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2+-mediated or apoptosis induced scramblase activity or externalization of phosphatidylserine.
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Exposure of endogenous phosphatidylserine at the outer surface of stimulated platelets is reversed by restoration of aminophospholipid translocase activity
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An excellent summary of the proteomic studies performed to date on human erythrocytes. This review also constructs and compares interactome networks for sickle and normal erythrocytes
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Morris CR, Suh JH, Hagar W, et al. Erythrocyte glutamine depletion, altered redox environment, and pulmonary hypertension in sickle cell disease. Blood 2008; 111:402-410. This publication reports data supporting the hypothesis that oxidative damage induced in red cells depletes glutamine and glutathione levels and is correlated with pulmonary hypertension in sickle cell disease. This study has implications for the mechanisms of the effects of oxidant inducing diseases on the structure of the red cell membrane.
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