Distribution of artemisinin and bioactive flavonoids from Artemisia annua L. during plant growth

Biochemical Systematics and Ecology

Volumn 36, Issue 5-6, 2008, Pages 340-348

  Baraldi, Rita ^a   Isacchi, Benedetta ^b   Predieri, Stefano ^a   Marconi, Giancarlo ^c   Vincieri, Franco Francesco ^b   Bilia, Anna Rita ^b  

^a CNR   (Italy)

^b UNIVERSITY OF FLORENCE   (Italy)

^c Istituto Per La Sintesi Organica E La Fotoreattività   (Italy)

Author keywords

Artemisia annua L. (Asteraceae); Artemisinin; Developmental stage; HPLC DAD MS; Inflorescence; Leaf; Polymethoxyflavonoid

Indexed keywords

BIOACTIVITY; DEVELOPMENTAL STAGE; DICOTYLEDON; DRUG; DRUG RESISTANCE; ECOTYPE; ESTER; FLOWERING; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MEDICINAL PLANT; PIGMENT;

ARTEMISIA ANNUA; ASTERACEAE;

EID: 41949101650     PISSN: 03051978     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bse.2007.11.002     Document Type: Article

References (41)

1. Abdin M.Z., Israr M., Rehman R.U., and Jain S.K. Artemisinin, a novel antimalarial drug: biochemical and molecular approach for enhanced production. Planta Med. 69 (2003) 289-299
2. Acton N., and Klayman D.L. Artemisitene, a new sesquiterpene lactone endoperoxide from Artemisia annua. Planta Med. 5 (1985) 441-442
3. van Agtmael M.A., Eggelte T.A., and van Boxtel C.J. Artemisinin drugs in the treatment of malaria: from medicinal herb to registered medication. Trends Pharmacol. Sci. 20 5 (1999) 199-205
4. Bilia A.R., Melillo de Malgalhaes P., Bergonzi M.C., and Vincieri F.F. Simultaneous analysis of artemisinin and flavonoids of several extracts of Artemisia annua L. obtained from a commercial sample and a selected cultivar. Phytomedicine 13 (2006) 487-493
5. Bilia A.R., Lazari D., Messori L., Taglioli V., Temperini C., and Vincieri F.F. Simple and rapid physico-chemical methods to examine action of antimalarial drugs: its application to Artemisia annua L. constituents. Life Sci. 70 (2002) 769-778
6. Bormann S., Szlezak N., Faucher J.F., Matsiegui P.B., Neubauer R., Biner R.K., Lell B., and Kremsner P.G. Artesunate and praziquantel for the treatment of Schistosoma haematobium infections: a double-blind randomized. Placebo-controlled study. J. Infect. Dis. 184 (2001) 1363-1366
7. Charles D.J., Simon J.E., Wood K.V., and Heinstein P. Germplasm variation in artemisinin content of Artemisia annua using an alternative method of artemisinin analysis from crude plant-extracts. J. Nat. Prod. 53 (1990) 157-160
8. Charles D.J., Cebert E., and Simon J.E. Characterization of the essential oil of Artemisia annua L. J. Essent. Oil Res. 3 (1991) 33-39
9. De Vries P.J., and Dien T.K. Clinical pharmacology and therapeutic potential of artemisinin and its derivatives in the treatment of malaria. Drugs 52 6 (1996) 818-836
10. Donrop A.M., and Day N.P.J. The treatment of severe malaria. Trans. R. Soc. Trop. Med. Hyg. 101 (2007) 633-634
11. Duke M.V., Paul R.N., ElSohly H.N., Sturtz G., and Duke S.O. Localization of artemisinin and artemisitene in foliar tissues of glanded and glandless biotype of Artemisia annua L. Int. J. Plant Sci. 155 (1994) 365-372
12. Efferth T., Dunstan H., Sauerbrey A., Miyachi H., and Chitambar C.R. The anti-malarial artesunate is also active against cancer. Int. J. Oncol. 18 767 (2001) 773
13. Elford B.C., Roberts M.F., Phillipson J.D., and Wilson R.J. Potentiation of the antimalarial activity of qinghaosu by methoxylated flavones. Trans. R. Soc. Trop. Med. Hyg. 81 (1987) 434-436
14. ElSohly H.N., Croom Jr. E.M., ElFeraly F.S., and ElSherei M.M. A large-scale extraction (technique of artemisinin from Artemisia annua. J. Nat. Prod. 53 (1990) 1560-1564
15. Ferreira J.F.S., and Janick J. Distribution of artemisinin Artemisia annua. In: Janick J. (Ed). Progress in New Crops (1996), ASHS Press, Arlington, VA 579-584
16. Ferreira J.F.S., Simon J.E., and Janik J. Developmental studies of Artemisia annua L.: flowering and artemisinin production under greenhouse and field conditions. Planta Med. 61 (1995) 167-170
17. Heppner D.G., and Ballou V.R. Malaria in 1998: advances in diagnosis, drugs and vaccine development. Curr. Opin. Infect. Dis. 11 5 (1998) 519-530
18. Hien T.T., and White N.J. Qinghaosu. Lancet 341 (1993) 603-608
19. Jain D.C., Mathur A.K., Gupta M.N., Singh A.K., Verma R.K., Gupta A.P., and Kumar S. Isolation of high artemisinin-yielding clones of Artemisia annua. Phytochemistry 43 (1996) 993-1001
20. Klaiman D.C. Quinghaosu (artemisinin): an antimalarial drug from China. Science 228 (1985) 1049-1055
21. Laughlin J.C. Effect of agronomic practices on plant yield and antimalarial constituents of Artemisia annua L. Acta Hortic. 331 (1993) 54-61
22. Laughlin J.C. The influence of distribution of antimalarial constituents in Artemisia annua L. on time and method of harvest. Acta Hortic. 390 (1995) 67-73
23. Laughlin J.C., Heazlewood G.N., and Beattie B.M. Cultivation of Artemisia annua L. In: Wright C.W. (Ed). Artemisia (2002), Taylor & Francis, London, N.Y. 160-195
24. Liersh R., Soicke H., Stehr C., and Tüllner H.U. Formation of artemisinin in Artemisia annua during one vegetation period. Planta Med. 52 (1986) 387-390
25. Morales M., Charles D.J., and Simon J.E. Seasonal accumulation of artemisinin in Artemisia annua L. Acta Hortic. 344 (1993) 416-420
26. Pras N., Visser J.F., Batterman S., Woerdenbag H.J., Malingrè T.M., and Lugt C.B. Laboratory selection of Artemisia annua L. for high artemisinin yielding types. Phytochem. Anal. 2 (1991) 80-83
27. Rapparini F., Llusià J., and Peñuelas J. Effect of arbuscular mycorrhizal (AM) colonization on terpene emission and content of Artemisia annua L. Plant Biol. (2007) 10.1055/s-2007-964963
28. RBM. Roll Back Malaria, Meeting on the Production of Artemisinin and ACTS. 2nd Draft Report. Available from: (2005). http://rbm.who.int/mmss/library.html
29. Romero M.R., Efferth T., Serrano M.A., Castano B., Macias R.I., Briz O., and Marin J.J. Effect of artemisinin/artesunate as inhibitors of hepatitis B virus production in an "in vitro" system. Antivir Res. 68 (2005) 75-83
30. Simon J.E., Charles E., Cebert L., Grant J., Janick J., and Whipkey A. Artemisia annua L.: a promising aromatic and medicinal. Advances in New Crops (1990), Timber Press, Portland, OR. pp. 522-526
31. Singh N.P., and Lai H. Selective toxicity of dihydroartemisinin and holotransferrin toward human breast cancer cells. Life Sci. 70 (2001) 49-56
32. Singh A., Vishwarkarma R.A., and Husain A. Evaluation of Artemisia annua strains for higher artemisinin production. Planta Med. 54 (1988) 475-476
33. Trigg P.I. Qinghaosu (artemisinin) as an antimalarial drug. Econ. Med. Plant Res. 3 (1990) 20-55
34. Utzinger J., Xiao S., N'Goran E.K., Berquist R., and Tanner M. The potential of artemether for the control of schistosomiasis. Int. J. Parasitol. 31 (2001) 1549-1562
35. Van Geldre E., Vergauwe A., and van den Eeckhout E. State of the production of the antimalarial compound artemisinin in plants. Plant Mol. Biol. 33 (1997) 199-209
36. Vaz A.P.A., Scaranari C., Batista L.A.R., Figueira G.M., Sartoratto A., and Magalhães P.M. Biomassa e composição química de genótipos melhorados de espécies medicinais cultivadas em quatro municípios paulistas. Pesqui. Agropecu. Bras. 41 (2006) 869-872
37. Wang C.W. The Forests of China, with a Survey of Grassland and Desert Vegetation vol. 5 (1961), Harvard University, Maria Moors Cabot Foundation, Cambridge, MA pp. 171-187
38. WHO. World Malaria Report. Available from: (2005). http://rbm.who.int/wmr2005/pdf/WMReport
39. Woerdenbag H.J., Pras N., Bos R., Visser J.F., Hendriks H., and Malingrè T.M. Analysis of artemisinin and related sesquiterpenoids from Artemisia annua L. by combined gas chromatography/mass spectrometry. Phytochem. Anal. 2 (1991) 215-219
40. Woerdenbag H.J., Lüers J.F.J., Van Uden W., Pras N., Malingrè T.M., and Alfermann A.W. Production of the new antimalarial drug in shoot cultures of Artemisia annua L. Plant Cell Tissue Organ Cult. 32 (1993) 247-257
41. Yang S.L., Roberts M.F., O'Neill M.J., Bucat F., and Phillipson J.D. Flavonoids and chromenes from Artemisia annua. Phytochemistry 38 (1995) 255-257