Susceptibility of ovine lens crystallins to proteolytic cleavage during formation of hereditary cataract

Investigative Ophthalmology and Visual Science

Volumn 49, Issue 3, 2008, Pages 1016-1022

  Robertson, Lucinda J G ^a   David, Larry L ^b   Riviere Phillip, Michael A ^b   Wilmarth, A ^b   Muir, Matthew S ^a   Morton, James D ^a  

^a LINCOLN UNIVERSITY   (New Zealand)

^b OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CALPAIN; COMPLEMENTARY DNA; CRYSTALLIN; ALPHA CRYSTALLIN; BETA CRYSTALLIN; GAMMA CRYSTALLIN;

ANIMAL TISSUE; ARTICLE; CATARACT; CONTROLLED STUDY; DNA SEQUENCE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DEGRADATION; SHEEP; TWO DIMENSIONAL ELECTROPHORESIS; ANIMAL; GENETICS; LENS; MASS SPECTROMETRY; METABOLISM; POLYMERASE CHAIN REACTION; TWO DIMENSIONAL GEL ELECTROPHORESIS;

ALPHA-CRYSTALLINS; ANIMALS; BETA-CRYSTALLINS; CALPAIN; CATARACT; DNA, COMPLEMENTARY; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; GAMMA-CRYSTALLINS; LENS, CRYSTALLINE; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; SHEEP; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 41949083110     PISSN: 01460404     EISSN: 15525783     Source Type: Journal    
DOI: 10.1167/iovs.07-0792     Document Type: Article

References (31)

1. Bloemendal H, de Jong W, Jaenicke R, et al. Ageing and vision: structure, stability and function of lens crystallins. Prog Biophys Mol Biol. 2004;86(3):407-485.
2. Hanson SR, Hasan A, Smith DL, Smith JB. The major in vivo modifications of the human water-insoluble lens crystallins are disulfide bonds, deamidation, methionine oxidation and backbone cleavage. Exp Eye Res. 2000;71(2):195-207.
3. Lapko VN, Smith DL, Smith JB. Methylation and carbamylation of human gamma-crystallins. Protein Sci. 2003;12(8):1762-1774.
4. Wilmarth PA, Tanner S, Dasari S, et al. Age-related changes in human crystallins determined from comparative analysis of posttranslational modifications in young and aged lens: does deamidation contribute to crystallin insolubility? J Proteome Res. 2006;5(10):2554-2566.
5. Kim YH, Kapfer DM, Boekhorst J, et al. Deamidation, but not truncation, decreases the urea stability of a lens structural protein, betaB1-crystallin. Biochemistry. 2002;41(47):14076-14084.
6. David LL, Shearer TR. Beta-crystallins insolubilized by calpain II in vitro contain cleavage sites similar to beta-crystallins insolubilized during cataract. FEBS Lett. 1993;324(3):265-270.
7. Shearer TR, Ma H, Shih M, et al. Lp82 calpain during rat lens maturation and cataract formation. Curr Eye Res. 1998;17(11):1037-1043.
8. Ueda Y, Fukiage C, Shih M, Shearer TR, David LL. Mass measurements of C-terminally truncated alpha-crystallins from two-dimensional gels identify Lp82 as a major endopeptidase in rat lens. Mol CellProteomics. 2002;1(5):357-365.
9. Ueda Y, McCormack AL, Shearer TR, David LL. Purification and characterization of lens specific calpain (Lp82) from bovine lens. Exp Eye Res. 2001;73(5):625-637.
10. Yoshida H, Yumoto N, Tsukahara I, Murachi T. The degradation of alpha-crystallin at its carboxyl-terminal portion by calpain in bovine lens. Invest Ophthalmol Vis Sci. 1986;27(8):1269-1273.
11. Shih M, David LL, Lampi KJ, et al. Proteolysis by m-calpain enhances in vitro light scattering by crystallins from human and bovine lenses. Curr Eye Res. 2001;22(6):458-469.
12. David LL, Shearer TR. Purification of calpain II from rat lens and determination of endogenous substrates. Exp Eye Res. 1986;42(3):227-238.
13. Nakajima E, Walkup RD, Ma H, Shearer TR, Azuma M. Low activity by the calpain system in primate lenses causes resistance to calcium-induced proteolysis. Exp Eye Res. 2006;83(3):593-601.
14. Robertson LJ, Morton JD, Yamaguchi M, et al. Calpain may contribute to hereditary cataract formation in sheep. Invest Ophthalmol Vis Sci. 2005;46(12):4634-4640.
15. Lampi KJ, Shih M, Ueda Y, Shearer TR, David LL. Lens proteomics: analysis of rat crystallin sequences and two-dimensional electrophoresis map. Invest Ophthalmol Vis Sci. 2002;43(1):216-224.
16. Nivinskas H, Cole KD. Environmentally benign staining procedure for electrophoresis gels using Coomassie Brilliant Blue. Biotechniques. 1996;20(3):380-382, 384-385.
17. Wilmarth PA, Taube JR, Riviere MA, Duncan MK, David LL. Proteomic and sequence analysis of chicken lens crystallins reveals alternate splicing and translations forms of beta B2 and beta A2 crystallins. Invest Ophthalmol Vis Sci. 2004;45(8):2705-2715.
18. Wang-Su ST, McCormack AL, Yang S, et al. Proteome analysis of lens epithelia, fibers, and the HLE B-3 cell line. Invest Ophthalmol Vis Sci. 2003;44(11):4829-4836.
19. Yates JR 3rd, Eng JK, McCormack AL, Schieltz D. Method to correlate tandem mass spectra of modified peptides to amino acid sequences in the protein database. Anal Chem. 1995;67(8):1426-1436.
20. Gasteiger E, Gattiker A, Hoogland C, et al. ExPASy: the proteomics server for in-depth protein knowledge and analysis. Nucleic Acids Res. 2003;31(13):3784-3788.
21. Hogan JM, Pitteri SJ, McLuckey SA. Phosphorylation site identification via ion trap tandem mass spectrometry of whole protein and peptide ions: bovine alpha-crystallin A chain. Anal Chem. 2003;75(23):6509-6516.
22. Shih M, Lampi KJ, Shearer TR, David LL. Cleavage of beta crystallins during maturation of bovine lens. Mol Vis. 1998;4:4.
23. Lampi KJ, Ma Z, Shih M, et al. Sequence analysis of betaA3, betaB3, and betaA4 crystallins completes the identification of the major proteins in young human lens. J Biol Chem. 1997;272(4):2268-2275.
24. David LL, Azuma M, Shearer TR. Cataract and the acceleration of calpain-induced beta-crystallin insolubilization occurring during normal maturation of rat lens. Invest Ophthalmol Vis Sci. 1994;35(3)785-793.
25. Ueda Y, Duncan MK, David LL. Lens proteomics: the accumulation of crystallin modifications in the mouse lens with age. Invest Ophthalmol Vis Sci. 2002;43(1):205-215.
26. Lampi KJ, Ma Z, Hanson SR, et al. Age-related changes in human lens crystallins identified by two-dimensional electrophoresis and mass spectrometry. Exp Eye Res. 1998;67(1):31-43.
27. Miesbauer LR, Zhou X, Yang Z, et al. Post-translational modifications of water-soluble human lens crystallins from young adults. J Biol Chem. 1994;269(17):12494-12502.
28. Wang K, Gawinowicz MA, Spector A. The effect of stress on the pattern of phosphorylation of alphaA and alphaB crystallin in the rat lens. Exp Eye Res. 2000;71(4):385-393.
29. Thampi P, Abraham EC. Influence of the C-terminal residues on oligomerization of alpha A-crystallin. Biochemistry. 2003;42(40):11857- 11863.
30. Tang Y, Liu X, Zoltoski RK, et al. Age-related cataracts in a3Cx46-knockout mice are dependent on a calpain 3 isoform. Invest Ophthalmol Vis Sci. 2007;48:2685-2694.
31. Sandberg HO, Closs O. The alpha and gamma crystallin content in aqueous humor of eyes with clear lenses and with cataracts. Exp Eye Res. 1979;28(5):601-610.