Characterization and epitope mapping of monoclonal antibodies to the nucleocapsid protein of severe acute respiratory syndrome coronavirus

Japanese Journal of Veterinary Research

Volumn 55, Issue 4, 2008, Pages 115-127

  Kariwa, Hiroaki ^a   Noda, Hiroshi ^a   Nakauchi, Mina ^a   Ishizuka, Mariko ^a   Hashiguchi, Kazuaki ^a   Hashimoto, Shingo ^a   Yoshii, Kentaro ^a   Asano, Atsushi ^a   Agui, Takashi ^a   Kogaki, Hiroyuki ^b   Kurano, Yoshihiro ^b   Uchida, Yoshiaki ^b   Fujii, Nobuyuki ^b   Okada, Masahisa ^b   Takashima, Ikuo ^a  

^a HOKKAIDO UNIVERSITY   (Japan)

^b FUJIREBIO INC   (Japan)

Author keywords

Coronavirus; Epitope; Monoclonal antibody; Nucleocapsid; Severe acute respiratory syndrome (SARS)

Indexed keywords

AMINO ACID; MONOCLONAL ANTIBODY; NUCLEOCAPSID PROTEIN; NUCLEOCAPSID PROTEIN, CORONAVIRUS; UNCLASSIFIED DRUG; VIRUS ANTIBODY;

AMINO ACID SEQUENCE; ANIMAL; ANIMAL DISEASE; ANTIBODY SPECIFICITY; ARTICLE; CERCOPITHECUS; CHEMISTRY; CORONAVIRUS; CROSS REACTION; EPITOPE MAPPING; FLUORESCENT ANTIBODY TECHNIQUE; GENETICS; HUMAN; IMMUNOLOGY; MOLECULAR CLONING; MOUSE; RABBIT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SARS CORONAVIRUS; SEVERE ACUTE RESPIRATORY SYNDROME; VERO CELL; WESTERN BLOTTING;

AMINO ACID SEQUENCE; AMINO ACIDS; ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODIES, VIRAL; ANTIBODY SPECIFICITY; BLOTTING, WESTERN; CERCOPITHECUS AETHIOPS; CLONING, MOLECULAR; CORONAVIRUS 229E, HUMAN; CROSS REACTIONS; EPITOPE MAPPING; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; HUMANS; MICE; NUCLEOCAPSID PROTEINS; RABBITS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SARS VIRUS; SEVERE ACUTE RESPIRATORY SYNDROME; VERO CELLS;

CORONAVIRUS; HUMAN CORONAVIRUS 229E; SARS CORONAVIRUS;

EID: 41749088437     PISSN: 00471917     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (29)

1. Drosten, C., Gunther, S., Preiser, W., van der Werf, S., Brodt, H. R., Becker, S., Rabenau, H., Panning, M., Kolesnikova, L., Fouchier, R. A., Berger, A., Burguiere, A. M., Cinatl, J., Eickmann, M., Escriou, N., Grywna, K., Kramme, S., Manuguerra, J. C., Muller, S., Rickerts, V., Sturmer, M., Vieth, S., Klenk, H. D., Osterhaus, A. D., Schmitz, H and Doerr, H. W. 2003. Identification of a novel coronavirus in patients with severe acute respiratory syndrome. N. Engl. J. Med., 348: 1967-1976.
2. Frohman, M. A., Dush, M. K. and Martin, G. R. 1988. Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc. Natl. Acad. Sci. U. S.A., 85: 8998-9002.
3. Fujimura, K., Ise, N., Ueno, E., Hori, T., Fujii, N. and Okada, M. 1997. Reactivity of recombinant Treponema pallidum (r-Tp) antigens with anti-Tp antibodies in human syphilitic sera evaluated by ELISA. J. Clin. Lab. Anal., 11: 315-322.
4. Guan, Y., Zheng, B. J., He, Y. Q., Liu, X. L., Zhuang, Z. X., Cheung, C. L., Luo, S. W., Li, P. H., Zhang, L. J., Guan, Y. J., Butt, K. M., Wong, K. L., Chan, K. W., Lim, W., Shortridge, K. F., Yuen, K. Y, Peiris, J. S. and Poon, L. L. 2003. Isolation and characterization of viruses related to the SARS coronavirus from animals in southern China. Science, 302: 276-278.
5. Guo, J. P., Petric, M., Campbell, W. and McGeer, P. L. 2004. SARS corona virus peptides recognized by antibodies in the sera of convalescent cases. Virology, 324: 251-256.
6. Hsieh, P. K., Chang, S. C., Huang, C. C., Lee, T. T., Hsiao, C. W., Kou, Y. H., Chen, I. Y., Chang, C. K., Huang, T. H. and Chang, M. F. 2005. Assembly of severe acute respiratory syndrome coronavirus RNA packaging signal into virus-like particles is nucleocapsid dependent. J. Virol, 79: 13848-13855.
7. Huang, Q., Yu. L., Petros, AM, Gunasekera, A., Liu, Z., Xu, N., Hajduk, P., Mack, J., Fesik, S. W. and Olejniczak, E. T. 2004. Structure of the N-terminal RNA-binding domain of the SARS CoV nucleocapsid protein. Biochemistry, 43: 6059-6063.
8. Kogaki, H., Uchida, Y., Fujii, N., Kurano, Y., Miyake, K., Kido, Y., Kariwa, H., Takashima, I., Tamashiro, H., Ling, A. E. and Okada, M. 2005. Novel rapid immunochromatographic test based on an enzyme immunoassay for detecting nucleocapsid antigen in SARS-associated coronavirus. J. Clin. Lab. Anal., 19: 150-159.
9. Krokhin, O., Li, Y., Andonov, A., Feldmann, H., Flick, R., Jones, S., Stroeher, U., Bastien, N., Dasuri, K. V., Cheng, K., Simonsen, J. N., Perreault, H., Wilkins, J., Ens, W., Plummer, F. and Standing, K. G. 2003. Mass spectrometric characterization of proteins from the SARS virus: a preliminary report. Mol. Cell Proteomics, 2: 346-356.
10. Ksiazek, T. G., Erdman, D., Goldsmith, C. S., Zaki, S. R., Peret, T., Emery, S., Tong, S., Urbani, C., Comer, J. A., Lim, W., Rollin, P. E., Dowell, S. F., Ling, A. E., Humphrey, C. D., Shieh, W. J., Guarner, J., Paddock, C. D., Rota, P., Fields, B., DeRisi, J., Yang, J. Y, Cox, N., Hughes, J. M., LeDuc, J. W., Bellini, W. J., Anderson, L. J.; SARS Working Group. 2003. A novel coronavirus associated with severe acute respiratory syndrome. N. Engl. J. Med, 348: 1953-1966.
11. Lau, S. K., Woo, P. C., Li, K. S., Huang, Y., Tsoi, H. W., Wong, B. H., Wong, S. S., Leung, S. Y., Chan, K. H. and Yuen, K. Y 2005. Severe acute respiratory syndrome coronavirus-like virus in Chinese horseshoe bats. Proc. Natl. Acad. Sci. U. S. A., 102: 14040-14045.
12. Leung, D. T., Tam, F. C., Ma, C. H., Chan, P. K., Cheung, J. L., Niu, H., Tarn, J. S. and Lim, P. L. 2004. Antibody response of patients with severe acute respiratory syndrome (SARS) targets the viral nucleocapsid. J. Infect. Dis., 190: 379-386.
13. Li, W., Shi, Z., Yu, M., Ren, W., Smith, C., Epstein, J. H., Wang, H., Crameri, G., Hu, Z., Zhang, H., Zhang, J., McEachern, J., Field, H., Daszak, P., Eaton, B. T., Zhang, S. and Wang, L. F. 2005. Bats are natural reservoirs of SARS-like coronaviruses. Science, 310: 676-679.
14. Luo, H., Chen, Q., Chen, J., Chen, K., Shen, X. and Jiang, H. 2005. The nucleocapsid protein of SARS coronavirus has a high binding affinity to the human cellular heterogeneous nuclear ribonucleoproteinvA1. FEBS Lett., 579: 2623-2628.
15. Luo, H., Ye, F., Chen, K., Shen, X. and Jiang, H. 2005. SR-rich motif plays a pivotal role in recombinant SARS coronavirus nucleocapsid protein multimerization. Biochemistry, 44: 15351-15358.
16. Marra, M. A., Jones, S. J., Astell, C. R., Holt, R. A., Brooks-Wilson, A., Butterfield, Y. S., Khattra, J., Asano, J. K., Barber, S. A., Chan, S. Y., Cloutier, A., Coughlin, S. M., Freeman, D., Girn, N., Griffith, O. L., Leach, S. R., Mayo, M., McDonald, H., Montgomery, S. B., Pandoh, P. K., Petrescu, A. S., Robertson, A. G., Schein, J. E., Siddiqui, A., Smailus, D. E., Stott, J. M., Yang, G. S., Plummer, F., Andonov, A., Artsob, H., Bastien, N., Bernard, K., Booth, T. F., Bowness, D., Czub, M., Drebot, M., Fernando, L., Flick, R., Garbutt, M., Gray, M., Grolla, A., Jones, S., Feldmann, H., Meyers, A., Kabani, A., Li, Y., Normand, S., Stroher, U., Tipples, G. A., Tyler, S., Vogrig, R., Ward, D., Watson, B., Brunham, R. C., Krajden, M., Petric, M., Skowronski, D. M., Upton, C. and Roper, R. L. 2003. The Genome sequence of the SARS-associated coronavirus. Science, 300: 1399-1404.
17. Ng, S. K. M. 2003. Possible role of an animal vector in the SARS outbreak at Amoy Gardens. Lancet, 362: 570-572.
18. Peiris, J. S., Yuen, K. Y., Osterhaus, A. D. and Stohr, K. 2003. The severe acute respiratory syndrome. N. Engl J. Med., 349: 2431-2441.
19. Poutanen, S. M., Low, D. E., Henry, B., Finkelstein, S., Rose, D., Green, K., Tellier, R., Draker, R., Adachi, D., Ayers, M., Chan, A. K., Skowronski, D. M., Salit, I., Simor, A. E., Slutsky, A. S., Doyle, P. W, Krajden, M., Petric, M., Brunham, R. C., McGeer, A. J.; National Microbiology Laboratory, Canada; Canadian Severe Acute Respiratory Syndrome Study Team. 2003. Identification of severe acute respiratory syndrome in Canada. N. Engl. J. Med., 348: 1995-2005.
20. Rosling, L. and Rosling, M. 2003. Pneumonia causes panic in Guangdong province. Br. Med. J., 326: 416.
21. Rota, P. A., Oberste, M. S., Monroe, S. S., Nix, W. A., Campagnoli, R., Icenogle, J. P., Penaranda, S., Bankamp, B., Maher, K., Chen, M. H., Tong, S., Tamin, A., Lowe, L., Frace, M., DeRisi, J. L., Chen, Q., Wang, D., Erdman, D. D., Peret, T. C., Burns, C., Ksiazek, T. G., Rollin, P. E., Sanchez, A., Liffick, S., Holloway, B., Limor, J., McCaustland, K., Olsen-Rasmussen, M., Fouchier, R., Gunther, S., Osterhaus, A. D., Drosten, C., Pallansch, M. A., Andersen, L. J. and Bellini, W. J. 2003. Characterization of a novel coronavirus associated with severe acute respiratory syndrome. Science, 300: 1394-1399.
22. Shi, Y., Yi, Y., Li, P., Kuang, T., Li, L., Dong, M., Ma, Q. and Cao, C. 2003. Diagnosis of severe acute respiratory syndrome (SARS) by detection of SARS coronavirus nucleocapsid anti-bodies in an antigen-capturing enzyme-linked immunosorbent assay. J. Clin. Microbiol., 41: 5781-5782.
23. Surjit, M., Liu, B., Kumar, P., Chow, V. T. and Lal, S. K. 2004. The nucleocapsid protein of the SARS coronavirus is capable of self-association through a C-terminal 209 amino acid interaction domain. Biochem. Biophys. Res. Commun., 317: 1030-1036.
24. Tan, Y. J., Goh, P. Y., Fielding, B. C., Shen, S., Chou, C. F., Fu, J. L., Leong, H. N., Leo, Y. S., Ooi, E. E., Ling, A. E., Lim, S. G. and Hong, W. 2004. Profiles of antibody responses against severe acute respiratory syndrome coronavirus recombinant proteins and their potential use as diagnostic markers. Clin. Diagn. Lab. Immunol., 11: 362-371.
25. Wang, J., Wen, J., Li, J., Yin, J., Zhu, Q., Wang, H., Yang, Y, Qin, E., You, B., Li, W, Li, X., Huang, S., Yang, R., Zhang, X., Yang, L., Zhang, T., Yin, Y, Cui, X., Tang, X., Wang, L., He, B., Ma, L., Lei, T., Zeng, C., Fang, J., Yu, J., Wang, J., Yang, H., West, M. B., Bhatnagar, A., Lu, Y., Xu, N. and Liu, S. 2003. Assessment of immunoreactive synthetic peptides from the structural proteins of severe acute respiratory syndrome coronavirus. Clin. Chem., 49: 1989-1996.
26. World Health Organization. 2003. Available at: http//who.int/csr/sars/ archive/2003_03_12/en/print.html
27. Yu, I. M., Gustafson, C. L., Diao, J., Burgner, J. W. 2nd, Li, Z., Zhang, J. and Chen, J. 2005. Recombinant severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein forms a dimer through its C-terminal domain. J. Biol. Chem., 280: 23280-23286.
28. Zhao, Z., Zhang, F., Xu, M., Huang, K., Zhong, W., Cai, W., Yin, Z., Huang, S., Deng, Z., Wei, M., Xiong, J. and Hawkey, P. M. 2003. Description and clinical treatment of an early outbreak of severe acute respiratory syndrome (SARS) in Guangzhou, PR China. J. Med. Microbiol., 52: 715-720.
29. Zhong, N. S., Zheng, B. J., Li, Y. M., Poon, L. L. M. Xie, Z. H., Chan, K. H., Li, P. H., Tan, S. Y., Chang, Q., Xie, J. P., Liu, X. Q., Xu, J, Li, D. X., Yuen, K. Y., Peiris, J. S. M. and Guan, Y. 2003. Epidemiology and cause of severe acute respiratory syndrome (SARS) in Guangdong, People's Republic of China, in February, 2003. Lancet., 362: 1353-1358.