First evidence of the pore-forming properties of a keratin from skin mucus of rainbow trout (Oncorhynchus mykiss, formerly Salmo gairdneri)

Biochemical Journal

Volumn 411, Issue 1, 2008, Pages 33-40

  Molle, Virginie ^a   Campagna, Sylvie ^b,c   Bessin, Yannick ^b,c   Ebran, Nathalie ^d   Saint, Nathalie ^b,c   Molle, Gérard ^b,c  

^a UNIVERSITÉ DE LYON   (France)

^b CENTRE DE BIOCHIMIE STRUCTURALE   (France)

^c INSERM   (France)

^d UNIVERSITY HOSPITAL OF NICE   (France)

Author keywords

Bilayer; Channel activity; Fish defence; Rainbow trout; Recombinant keratin; Skin mucous layer

Indexed keywords

BILAYERS; CHANNEL ACTIVITY; FISH DEFENCE; RAINBOW TROUT; RECOMBINANT KERATIN; SKIN MUCOUS LAYERS;

BACTERIA; DNA SEQUENCES; FISH; FUNGI; GLYCOPROTEINS;

PROTEINS;

COMPLEMENTARY DNA; CYTOKERATIN 2; KERATIN; PROTEIN; TR65 PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; ENZYMATIC ASSAY; EPIDERMIS; GENE CONSTRUCT; MOLECULAR CLONING; MUCOSA; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN FUNCTION; RAINBOW TROUT; SEQUENCE HOMOLOGY;

ANIMALS; BASE SEQUENCE; CLONING, MOLECULAR; DNA, COMPLEMENTARY; EPIDERMIS; KERATINS; MUCUS; ONCORHYNCHUS MYKISS; PEPTIDE FRAGMENTS; POROSITY; SKIN;

ANIMALIA; FUNGI; ONCORHYNCHUS MYKISS; SALMONIDAE;

EID: 41649084938     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20070801     Document Type: Article

References (39)

1. Ellis, A. E. (1999) Immunity to bacteria in fish. Fish Shellfish Immunol. 9, 291-308
2. Ellis, A. E. (2001) Innate host defense mechanisms of fish against viruses and bacteria. Dev Comp. Immunol. 25, 827-839
3. Magnadottir, B. (2006) Innate immunity of fish (overview). Fish Shellfish Immunol. 20, 137-151
4. Alexander, J. B. and Ingram, G. A. (1992) Non-cellular non-specific defense mechanisms of fish. Annu. Rev. Fish Dis. 2, 249-279
5. Kaattari, S. L. and Piganelli, J. D. (1996) The specific immune system: humoral defense. In The Fish Immune System: Organism, Pathogen and Environment (Iwama, G. and Nakanishi, T., eds.), pp. 207-254, Fish Physiology Series, Academic Press, San Diego, CA, U.S.A.
6. Tatner, M. F. (1986) The ontogeny of humoral immunity in rainbow trout, Salmo gairdneri. Vet. Immunol. Immunopathol. 12, 93-105
7. Le Morvan, C., Deschaux, P. and Troutaud, D. (1996) Effects and mechanisms of environmental temperature on carp (Cyprinus carpio) anti-DNP antibody response and non-specific cytotoxic cell activity: a kinetic study. Dev. Comp. Immunol. 20, 331-340
8. Le Morvan, C., Troutaud, D. and Deschaux, P. (1998) Differential effects of temperature on specific and nonspecific immune defences in fish. J. Exp. Biol. 201, 165-168
9. Russell, S. and Lumsden, J. S. (2005) Function and heterogeneity of fish lectins. Vet. Immunol Immunopathol. 108, 111-120
10. Yano, T. (1996) The nonspecific immune system: humoral defense. In The Fish Immune System: Organism, Pathogen and Environment (Iwama, G. and Nakanishi, T., eds.), pp. 105-157, Academic Press, San Diego, CA, U.S.A.
11. Adermann, K., Raida, M., Paul, Y., Abu-Raya, S., Bloch-Shilderman, E., Lazarovici, P., Hochman, J. and Wellhoner, H. (1998) Isolation, characterization and synthesis of a novel paradaxin isoform. FEBS Lett. 435, 173-177
12. Lazarovici, P., Primor, N. and Loew, L. M. (1986) Purification and pore-forming activity of two hydrophobic polypeptides from the secretion of the Red Sea Moses sole (Pardachirus marmoratus). J. Biol. Chem. 261, 16704-16713
13. Cole, A. M., Weis, P. and Diamond, G. (1997) Isolation and characterization of pleurocidin, an antimicrobial peptide in the skin secretions of winter flounder. J. Biol. Chem. 272, 12008-12013
14. Shiomi, K., Igarashi, T., Yokota, H., Nagashima, Y. and Ishida, M. (2000) Isolation and structures of grammistins, peptide toxins from the skin secretion of the soapfish Grammistes sexlineatus. Toxicon 38, 91-103
15. Shiomi, K., Yokota, H., Nagashima, Y. and Ishida, M. (2001) Primary and secondary structures of grammistins, peptide toxins isolated from the skin secretion of the soapfish Pogonoperca punctata. Fisheries Sci. 67, 163-169
16. Sugiyama, N., Araki, M., Ishida, M., Nagashima, Y. and Shiomi, K. (2005) Further isolation and characterization of grammistins from the skin secretion of the soapfish Grammistes sexlineatus. Toxicon 45, 595-601
17. Park, I. Y., Park, C. B., Kim, M. S. and Kim, S. C., (1998) Parasin I, an antimicrobial peptide derived from histone H2A in the catfish, Parasilurus asotus. FEBS Lett. 437, 258-262
18. Fernandes, J. M., Kemp, G. D., Molle, M. G. and Smith, V. J. (2002) Anti-microbial properties of histone H2A from skin secretions of rainbow trout, Oncorhynchus mykiss. Biochem. J. 368, 611-620
19. Fernandes, J. M., Molle, G., Kemp, G. D. and Smith, V. J. (2004) Isolation and characterisation of oncorhyncin II, a histone H1-derived antimicrobial peptide from skin secretions of rainbow trout, Oncorhynchus mykiss. Dev. Comp. Immunol. 28, 127-138
20. Birkemo, G. A., Luders, T., Andersen, O., Nes, I. F. and Nissen-Meyer, J. (2003) Hipposin, a histone-derived antimicrobial peptide in Atlantic halibut (Hippoglossus hippoglossus L.). Biochim. Biophys. Acta 1646, 207-215
21. Ebran, N., Julien, S., Orange, N., Auperin, B. and Mollen, G. (2000) Isolation and characterization of novel glycoproteins from fish epidermal mucus: correlation between their pore-forming properties and their antibacterial activities. Biochim. Biophys. Acta 1467, 271-280
22. Ebran, N., Julien, S., Orange, N., Saglio, P., Lemaitre, C., and Molle, G. (1999) Pore-forming properties and antibacterial activity of proteins extracted from epidermal mucus of fish. Comp. Biochem. Physiol. A Mol. Integr. Physiol. 122, 181-189
23. Vilain, S., Cosette, P., Charlionet, R., Hubert, M., Lange, C. Junter, G. A. and Jouenne, T. (2001) Substituting Coomassie Brilliant Blue for bromophenol blue in two-dimensional electrophoresis buffers improves the resolution of focusing patterns. Electrophoresis 22, 4368-4374
24. Prilipov, A., Phale, P. S., Van Gelder, P., Rosenbusch, J. P. and Koebnik, R. (1998) Coupling site-directed mutagenesis with high-level expression: large scale production of mutant porins from E. coli. FEMS Microbiol. Lett. 163, 65-72
25. Siroy, A., Molle, V., Lemaitre-Guillier, C., Vallenet, D., Pestel-Caron, M., Cozzone, A. J., Jouenne, T. and De, E. (2005) Channel formation by CarO, the carbapenem resistance-associated outer membrane protein of Acinetobacter baumannii. Antimicrob. Agents Chemother 49, 4876-4883
26. Montal, M. and Mueller, P. (1972) Formation ot bimolecular membranes from lipid monolayers and a study of their electrical properties. Proc Natl. Acad. Sci. U.S.A. 69, 3561-3566
27. Molle, V., Brown, A. K., Besra, G. S., Cozzone, A. J. and Kremer, L. (2006) The condensing activities of the Mycobacterium tuberculosis type II fatty acid synthase are differentially regulated by phosphorylation. J. Biol. Chem. 281, 30094-30103
28. Lowthert, L. A., Ku, N. O., Liao, J., Coulombe, P. A. and Omary, M. B. (1995) Empigen BB: a useful detergent for solubilization and biochemical analysis of keratins. Biochem. Biophys. Res. Commun. 206, 370-379
29. Conrad, M., Lemb, K., Schubert, T. and Markl, J. (1998) Biochemical identification and tissue-specific expression patterns of keratins in the zebrafish Danio rerio. Cell Tissue Res. 293, 195-205
30. Easy, R. H. and Ross, N. W. (2006) Proteomic analysis of the epidermal mucus of Atlantic salmon (Salmon salar): biological indicators of stress. In 7th International Congress on the Biology of Fish (MacKinlay, C. and Busby, C., eds.), p. 90. St. John's, NL, Canada
31. Chong, K., Ying, T. S., Foo, J., Jin, L. T. and Chong, A. (2005) Characterisation of proteins in epidermal mucus of discus fish (Symphosodon spp.) during parental phase Aquaculture 249, 469-476
32. Koch, E. A., Spitzer, R. H., Pithawalla, R. B., Castillos, III, F. A. and Parry, D. A. (1995) Hagfish biopolymer: a type I/type II homologue of epidermal keratin intermediate filaments. Int. J. Biol. Macromol. 17, 283-292
33. Weiger, T.M., Hermann, A. and Levitan, I. B. (2002) Modulation of calcium-activated potassium channels. J. Comp. Physiol. A Neuroethol. Sens. Neural Behav. Physiol. 188, 79-87
34. Sivilotti, L. G., McNeil, D. K., Lewis, T. M., Nassar, M. A., Schoepfer, R. and Colquhoun, D. (1997) Recombinant nicotinic receptors, expressed in Xenopus oocytes, do not resemble native rat sympathetic ganglion receptors in single-channel behaviour. J. Physiol. 500, 123-138
35. Moreno, A. P. and Lau, A. F. (2007) Gap junction channel gating modulated through protein phosphorylation. Prog. Biophys. Mol. Biol. 94, 107-119
36. Schmieder, S., Bogliolo, S., and Ehrenfeld, J. (2007) N-glycosylation of the Xenopus laevis ClC-5 protein plays a role in cell surface expression, affecting transport activity at the plasma membrane. J. Cell. Physiol. 210, 479-488
37. Qi, W. and Boyao, W. (1999) Defense mechanisms of urinary bladder: studies on antimicrobial polypeptides from bladder mucosa. Chin. Med. Sci. J. 14, 17-22
38. Vos, J. B., van Sterkenburg, M. A., Rabe, K. F., Schalkwijk, J., Hiemstra, P. S. and Datson, N. A. (2005) Transcriptional response of bronchial epithelial cells to Pseudomonas aeruginosa: identification of early mediators of host defense. Physiol. Genomics 21, 324-336
39. Schaffeld, M. Haberkamp, M., Braziulis, E., Lieb, B. and Markl, J. (2002) Type II keratin cDNAs from the rainbow trout: implications for keratin evolution. Differentiation 70, 292-299