Recombinant nicotinic receptors, expressed in Xenopus oocytes, do not resemble native rat sympathetic ganglion receptors in single-channel behaviour

Journal of Physiology

Volumn 500, Issue 1, 1997, Pages 123-138

  Sivilotti, L G ^a   McNeil, D K ^a   Lewis, T M ^a   Nassar, M A ^a   Schoepfer, R ^a   Colquhoun, D ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

NICOTINIC RECEPTOR; RECEPTOR SUBUNIT; RECOMBINANT PROTEIN;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; FEMALE; ION CONDUCTANCE; KINETICS; NEWBORN; NONHUMAN; OOCYTE; PATCH CLAMP; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN MODIFICATION; PROTEIN QUATERNARY STRUCTURE; SUPERIOR CERVICAL GANGLION; SYMPATHETIC GANGLION; XENOPUS;

EID: 0030902282     PISSN: 00223751     EISSN: None     Source Type: Journal    
DOI: 10.1113/jphysiol.1997.sp022004     Document Type: Article

References (44)

1. AMADOR, M. & DANI, J. A. (1995). Mechanism for modulation of nicotinic acetylcholine receptors that can influence synaptic transmission. Journal of Neuroscience 15, 4525-4532.
2. ANAND, R., CONROY, W. G., SCHOEPFER, R., WHITING, P. & LINDSTROM, J. (1991). Neuronal nicotinic acetylcholine receptors expressed in Xenopus oocytes have a pentameric quaternary structure. Journal of Biological Chemistry 266, 11192-11198.
3. ARUNLAKSHANA, O. & SCHILD, H. O. (1959). Some quantitative uses of drug antagonists. British Journal of Pharmacology and Chemotherapy 14, 47-58.
4. BULLER, A. L. & WHITE, M. M. (1990). Altered patterns of N-linked glycosylation of the Torpedo acetylcholine receptor expressed in Xenopus oocytes. Journal of Membrane Biology 115, 1-11.
5. COHEN, B. N., FIGL, A., QUICK, M. W., LABARCA, C., DAVIDSON, N. & LESTER, H. A. (1995). Regions of β2 and β4 responsible for differences between the steady state dose-response relationships of the α3β2 and α3β4 neuronal nicotinic receptors. Journal of General Physiology 105, 745-764.
6. COLQUHOUN, D. (1971). Lectures on Biostatistics. Clarendon Press, Oxford.
7. COLQUHOUN, D. & SIGWORTH, F. J. (1995). Fitting and statistical analysis of single-channel records. In Single-Channel Recording, ed. SAKMANN, B. & NEHER, E., pp. 483-587. Plenum Press, New York.
8. CONROY, W. G. & BERG, D. K. (1995). Neurons can maintain multiple classes of nicotinic receptors distinguished by different subunit compositions. Journal of Biological Chemistry 270, 4424-4431.
9. COOPER, E., COUTURIER, S. & BALLIVET, M. (1991). Pentameric structure and subunit stoichiometry of a neuronal nicotinic acetylcholine receptor. Nature 350, 235-238.
10. CORRIVEAU, R. A. & BERG, D. K. (1993). Coexpression of multiple acetylcholine receptor genes in neurons: quantification of transcripts during development. Journal of Neuroscience 13, 2662-2671.
11. COVERNTON, P. J. O., KOJIMA, H., SIVILOTTI, L. G., GIBB, A. J. & COLQUHOUN, D. (1994). Comparison of neuronal nicotinic receptors in rat sympathetic neurones with subunit pairs expressed in Xenopus oocytes. Journal of Physiology 481, 27-34.
12. EDMONDS, B., GIBB, A. J. & COLQUHOUN, D. (1995). Mechanisms of activation of muscle nicotinic acetylcholine receptors, and the time course of endplate currents. Annual Review of Physiology 57, 469-493.
13. EDWARDS, F. A., KONNERTH, A., SAKMANN, B. & TAKAHASHI, T. (1989). A thin slice preparation for patch clamp recordings from synaptically connected neurones of the mammalian central nervous system. Pflügers Archiv 414, 600-612.
14. GIBB, A. J., KOJIMA, H., CARR, J. A. & COLQUHOUN, D. (1990). Expression of cloned receptor subunits produces multiple receptors. Proceedings of the Royal Society of London B 242, 108-112.
15. HOFFMAN, P. W., RAVINDRAN, A. & HUGANIR, R. L. (1994). Role of phosphorylation in desensitization of acetylcholine receptors expressed in Xenopus oocytes. Journal of Neuroscience 14, 4185-4195.
16. KLIMASCHEWSKI, L., REUSS, S., SPESSERT, R., LOBRON, C., WEVERS, A., HEYM, C., MAELICKE, A. & SCHRODER, H. (1994). Expression of nicotinic acetylcholine receptors in the rat superior cervical ganglion on mRNA and protein level. Molecular Brain Research 27, 167-173.
17. KRIEG, P. A. & MELTON, D. A. (1984). Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs. Nucleic Acids Research 12, 7057-7070.
18. KULLBERG, R., OWENS, J. L., CAMACHO, P., MANDEL, G. & BREHM, P. (1990). Multiple conductance classes of mouse nicotinic acetylcholine receptors expressed in Xenopus oocytes. Proceedings of the National Academy of Sciences of the USA 87, 2067-2071.
19. LISTERUD, M., BRUSSAARD, A. B., DEVAY, P., COLMAN, D. R. & ROLE, L. W. (1991). Functional contribution of neuronal AChR subunits revealed by antisense oligonucleotides. Science 254, 1518-1521. (See erratum in Science (1992) 255, 12.)
20. LUETJE, C. W. & PATRICK, J. (1991). Both α- and β-subunits contribute to the agonist sensitivity of neuronal nicotinic acetylcholine receptors. Journal of Neuroscience 11, 837-845.
21. MCGEHEE, D. S. & ROLE, L. W. (1995). Physiological diversity of nicotinic acetylcholine receptors expressed by vertebrate neurons. Annual Review of Physiology 57, 521-546.
22. MANDELZYS, A., DE KONINCK, P. & COOPER, E. (1995). Agonists and toxin sensitivities of ACh-evoked currents on neurons expressing multiple nicotinic ACh receptor subunits. Journal of Neurophysiology 74, 1212-1221.
23. MANDELZYS, A., PIE, B., DENERIS, E. S. & COOPER, E. (1994). The developmental increase in ACh current densities on rat sympathetic neurons correlates with changes in nicotinic ACh receptor α-subunit gene expression and occurs independent of innervation. Journal of Neuroscience 14, 2357-2364.
24. MATHIE, A., COLQUHOUN, D. & CULL-CANDY, S. G. (1991). Conductance and kinetic properties of single channel currents through nicotinic acetylcholine receptor channels in rat sympathetic ganglion neurones. Journal of Physiology 439, 717-750.
25. METHFESSEL, C., WITZEMANN, V., TAKAHASHI, T., MISHINA, M., NUMA, S. & SAKMANN, B. (1986). Patch clamp measurements on Xenopus laevis oocytes: currents through endogenous channels and implanted acetylcholine receptors and sodium channels. Pflügers Archiv 407, 577-588.
26. MISHINA, M., TARAI, T., IMOTO, K., NODA, M., TAKAHASHI, T., NUMA, S., METHFESSEL, C. & SAKMANN, B. (1986). Molecular distinction between fetal and adult forms of muscle acetylcholine receptor. Nature 321, 406-411.
27. PAPKE, R. L. (1993). The kinetic properties of neuronal nicotinic receptors: genetic basis of functional diversity. Progress in Neurobiology 41, 509-531.
28. PAPKE, R. L., BOULTER, J., PATRICK, J. & HEINEMANN, S. (1989). Single channel currents of rat neuronal nicotinic acetylcholine receptors expressed in Xenopus laevis oocytes. Neuron 3, 589-596.
29. PATON, W. D. M. & ZAIMIS, E. J. (1949). The pharmacological actions of polymethylene bis trimethylammonium salts. British Journal of Pharmacology 4, 381-400.
30. PUGH, P. C., CORRIVEAU, R. A., CONROY, W. G. & BERG, D. K. (1995). Novel subpopulation of neuronal acetylcholine receptors among those binding α-bungarotoxin. Molecular Pharmacology 47, 717-725.
31. RAMIREZ-LATORRE, J., YU, C. R., QU, X., PERIN, F., KARLIN, A. & ROLE, L. (1996). Functional contributions of α5 subunit to neuronal acetylcholine receptor channels. Nature 380, 347-351.
32. RUST, G., BURGUNDER, J. M., LAUTERBURG, T. E. & CACHELIN, A. B. (1994). Expression of neuronal nicotinic acetylcholine receptor subunits genes in the rat autonomic nervous system. European Journal of Neuroscience 6, 478-485.
33. SIGWORTH, F. J. & SINE, S. M. (1987). Data transformations for improved display and fitting of single-channel dwell time histograms. Biophysical Journal 52, 1047-1054.
34. STERN, P., BÉHÉ, P., SCHOEPFER, R. & COLQUHOUN, D. (1992). Single channel conductances of NMDA receptors expressed from cloned cDNAs: comparison with native receptors. Proceedings of the Royal Society of London B 250, 271-277.
35. STERN, P., CIK, M., COLQUHOUN, D. & STEPHENSON, F. A. (1994). Single channel properties of cloned NMDA receptors in HEK 293 cells: comparison with results from Xenopus oocytes. Journal of Physiology 476, 391-397.
36. TROUSLARD, J., MARSH, S. J. & BROWN, D. A. (1993). Calcium entry through nicotinic and calcium channels in cultured rat superior cervical ganglion cells. Journal of Physiology 468, 53-71.
37. 3 receptor ligand-gated ion channels. Receptors and Channels 3, 7-12.
38. VERNALLIS, A. B., CONROY, W. G. & BERG, D. K. (1993). Neurons assemble acetylcholine receptors with as many as three kinds of subunits while maintaining subunit segregation among receptor subtypes. Neuron 10, 451-464.
39. WANG, F., GERZANICH, V., WELLS, G. B., ANAND, R., PENG, X., KEYSER, K. & LINDSTROM, J. (1996). Assembly of human neuronal nicotinic receptor α5 subunits with α3, α2, and β4 subunits. Journal of Biological Chemistry 271, 17656-17665.
40. WONG, E. T., HOLSTAD, S. G., MENNERICK, S. J., HONG, S. E., ZORUMSKI, C. F. & ISENBERG, K. E. (1995). Pharmacological and physiological properties of a putative ganglionic nicotinic receptor, α3β4, expressed in transfected eucarvotic cells. Molecular Brain Research 28, 101-109.
41. WYLLIE, D. J. A., BÉHÉ, P., NASSAR, M., SCHOEPFER, R. & COLQUHOUN, D. (1996). Single-channel currents from recombinant NMDA NR1a/NR2D receptors expressed in Xenopus oocytes. Proceedings of the Royal Society of London B 263, 1079-1086.
42. YU, X.-M. & HALL, Z. W. (1991). Extracellular domains mediating epsilon subunit interactions of muscle acetylcholine receptor. Nature 352, 64-67.
43. ZOLI, M., LE NOVÈRE, N., HILL, J. A. JR & CHANGEUX, J.-P. (1995). Developmental regulation of nicotinic ACh receptor subunit mRNAs in the rat central and peripheral nervous systems. Journal of Neuroscience 15, 1912-1939.
44. ZWART, R., OORTGIESEN, M. & VIJVERBERG, H. P. M. (1995). Differential modulation of α3β2 and α3β4 neuronal nicotinic receptors expressed in Xenopus oocytes by flufenamic acid and niflumic acid. Journal of Neuroscience 15, 2168-2178.