Purification and characterization of an alkaline pectin lyase from Aspergillus flavus

Process Biochemistry

Volumn 43, Issue 5, 2008, Pages 547-552

  Yadav, Sangeeta ^a   Yadav, Pramod Kumar ^a   Yadav, Dinesh ^a   Yadav, Kapil Deo Singh ^a  

^a D D U GORAKHPUR UNIVERSITY   (India)

Author keywords

Aspergillus flavus; Characterization; Pectin lyase; Pectinase; Purification; Retting

Indexed keywords

ALKALINITY; AMMONIUM COMPOUNDS; ION CHROMATOGRAPHY; PH EFFECTS; PURIFICATION;

ASPERGILLUS FLAVUS; PECTIN LYASE;

ENZYME ACTIVITY;

ASPERGILLUS FLAVUS; CROTALARIA; CROTALARIA JUNCEA;

EID: 41549126253     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2008.01.015     Document Type: Article

References (34)

1. Lozano P., Manjon A., Iborra J.L., and Galvez D.M. Characteristics of the immobilized pectin lyase activity from a commercial pectolytic enzyme preparation. Acta Biotechnol 10 (1990) 531-539
2. Alkorta I., Garbisu C., Liama M.J., and Serra J.L. Industrial application of pectic enzymes: a review. Process Biochem 33 (1998) 21-28
3. Blanco P., Sieiro C., and Villa T.G. Production of pectic enzymes in yeast. FEMS Microbiol Lett 175 (1999) 1-9
4. Hoondal G.S., Tewari R.P., Tewari R., Dahiya N., and Beg Q.K. Microbiol alkaline pectinases and their industrial applications. A review. Appl Microbiol Biotechnol 59 (2002) 409-418
5. Delgado L., Blanca A.T., Huitron C., and Aguilar G. Pectin lyase from Aspergillus sp. CH-Y-1043. Appl Microbiol Biotechnol 39 (1992) 515-519
6. Targano V.M., and Pelosof A.M.R. Application of Doehlert design for water activity, pH and fermentation time, optimization for Aspergillus niger pectinolytic activity and production in solid state and submerged fermentations. Enzyme Microbiol Technol 25 (1994) 411-419
7. Satyanarayana N.G., and Kumar S. Microbial pectic transeliminases: a review. Biotechnol Lett 27 (2005) 451-458
8. Yadav S., Yadav P.K., and Yadav K.D.S. Pectin lyases of a few indigenous fungal strains. J Sci Ind Res 66 (2007) 601-604
9. Cappuccino J.G., and Sherman N. Microbiology: a laboratory manual. 5th ed. (1998), Benjamin/Cumming, California pp. 14-19
10. Molina S.M.G., Pelissari F.A., and Vitorello C.B.M. Screening and genetic improvement of pectinolytic fungi for degumming of textile fibres. Braz J Microbiol 32 (2001) 320-326
11. Albersheim P. Pectin lyase from fungi. Methods Enzymol 8 (1966) 628-631
12. Lowry O.H., Rose Brough N.J., Farr A.L., and Randall R.J. Protein measurement with Folin phenol reagent. J Biol Chem 193 (1951) 265-275
13. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
14. Werber K., and Osborn M. The relative mobility or molecular weight determination by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem 244 (1969) 4406-4412
15. Engel P.C. Enzyme kinetics. A steady state approach (1977), Chapman and Hall, London p.14-25
16. Zhang J., Henriksson H., Szabo I.J., Henriksson G., and Johansson G. The active component in the flax retting system of the zygomycetes Rhizopus oryzae sb is a family 28 polygalacturaonase. J Ind Microbiol Biotechnol 32 (2005) 431-438
17. Baciu I.E., and Jordening H.J. Kinetics of galacturonic acid release from sugar-beet pulp. Enz Microbiol Technol 34 (2004) 505-512
18. Alana A., Maria J.L., and Serra J.L. Purification and some properties of pectin lyase from Penicillium italicum. FEBS 280 (1991) 335-340
19. Silva D.O., Attwod M.M., and Tempest D.W. Partial purification and properties of pectin lyase from Penicillium expansum. World J Microbiol Biotechnol 9 (1993) 574-578
20. Chen W.C., Hsieh H.J., and Tseng T.C. Purification and characterization of a pectin lyase from Pythium splendens infected cucumber fruits. Bot Bull Acad Sin 38 (1998) 181-186
21. Adejuwon A.O., and Olutiola P.O. Pectin lyase activity in culture medium of Lasidioplodia theobromae. J Plant Sci 2 (2007) 334-340
22. Spagana G., Pifferi P.G., and Gilioli E. Immobilization of a pectin lyase from Aspergillus niger for application in food technology. Enzyme Microbiol Technol 17 (1995) 729-738
23. Yadav S., and Shastri N.V. Purification and properties of an extracellular pectin lyase produced by the strain of Penicillium oxalicum in solid-state fermentation. Indian J Biochem Biophys 44 (2007) 247-251
24. Sakiyama C.C.H., Paula E.M., Pereira P.C., Borges A.C., and Silva D.O. Characterization of pectin lyase produced by an endophytic strain isolated from coffee cherries. Lett Appl Microbiol 33 (2001) 117-121
25. Hamdy H.S. Purification and characterization of the pectin lyase produced by Rhizopus oryzae grown on orange peels. Ann Microbiol 55 (2005) 205-211
26. Sinitsyna O.A., Fedorova E.A., Semenova M.V., Gusakov A.V., Sokolova L.M., Bubnova T.M., et al. Isolation and characterization of extracellular pectin lyase from Penicillium canescens. Biochemistry (Moscow) 72 (2007) 565-571
27. Nakagawa T., Nagaoka T., Miyaji T., and Tomizuka N. A cold active pectin lyase from psychrophilic and basidiomycetous yeast Cystofilobasidium capitatum strain PPY-1. Biotechnol Appl Biochem 42 (2005) 193-196
28. Dinnella C., Lanzarini G., and Stagni A. Immobilization of an endopectin lyase on γ-alumina: study of factors influencing biocatalytic matrix stability. J Chem Technol Biotechnol 59 (1994) 237-241
29. Sapunova L.I., Mikhailova R.V., and Lobanok A.G. Properties of pectin lyase preparations from the genus Penicillium. Appl Microbiol Biochem 31 (1995) 435-438
30. Soriano M., Blanco A., Diaz P., and Pastor F.I.J. An unusual pectate lyase from Bacillus sp. with high activity on pectin: cloning and characterization. Microbiology 146 (2000) 89-95
31. Scopes R.K. Protein purification; principle and practice (1993), Springer Verlag, New York
32. Mayans O., Scott M., Connerton I., Gravesen T., Benen J., Visser J., et al. Two crystal structures of pectin lyase A from Aspergillus reveal a pH driven conformational change and striking divergence in the substrate-binding clefts of pectin and pectate lyases. Structure 5 (1997) 677-689
33. Zhang J., Henriksson G., and Johansson G. Polygalacturonase is the key enzyme component in enzyme retting of flax. J Biotechnol 81 (2000) 85-89
34. Henriksson G., Akin D.E., Rigsby L.L., Patel N., and Eriksson K.E.L. Influence of chelating agents and mechanical pretreatment on enzymatic retting of flax. Tex Res J 67 (1997) 829-836