Inhibition of human immunodeficiency virus type 1 activity by purified human breast milk mucin (MUC1) in an inhibition assay

Neonatology

Volumn 93, Issue 3, 2008, Pages 162-170

  Habte, Habtom H ^a   De Beer, Corena ^b   Lotz, Zoë E ^a   Tyler, Marilyn G ^a   Kahn, Delawir ^a   Mall, Anwar S ^a,c  

^a UNIVERSITY OF CAPE TOWN   (South Africa)

^b STELLENBOSCH UNIVERSITY   (South Africa)

^c UNIVERSITY OF CAPE TOWN   (South Africa)

Author keywords

Anti HIV 1 activity; Breast milk; CEM SS cells; HIV inhibition assay; MUC1 mucin; Phytohaemagglutinin

Indexed keywords

CESIUM CHLORIDE; MUCIN 1; SEPHAROSE;

AMINO ACID ANALYSIS; AMINO ACID COMPOSITION; ANTIVIRAL ACTIVITY; ARTICLE; BREAST FEEDING; BREAST MILK; COLUMN CHROMATOGRAPHY; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; PRIORITY JOURNAL; PROTEIN ISOLATION; PROTEIN PURIFICATION; T LYMPHOBLAST; VIRUS INHIBITION; VIRUS TRANSMISSION; WESTERN BLOTTING;

AMINO ACIDS; BLOTTING, WESTERN; CA-15-3 ANTIGEN; CELL LINE; CHROMATOGRAPHY, GEL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FEMALE; HIV INFECTIONS; HIV-1; HUMANS; MILK, HUMAN; ULTRACENTRIFUGATION;

HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 41349102036     PISSN: 16617800     EISSN: 16617819     Source Type: Journal    
DOI: 10.1159/000108414     Document Type: Article

References (43)

1. Losina E, Anglaret X, Yazdanpanah Y, Wang B, Toure S, Seage GR 3rd, N'Dri-Yoman T, Walensky RP, Dakoury-Dogbo N, Goldie SJ, Messou E, Weinstein MC, Deuffic-Burban S, Salamon R, Freedberg KA: Impact of opportunistic diseases on chronic mortality in HIV-infected adults in Côte d'Ivoire. S Afr Med J 2006;96:526-529.
2. Shaikh N, Abdullah F, Lombard CJ, Smit L, Bradshaw D, Makubalo L: Masking through averages - intraprovincial heterogeneity in HIV prevalence within the Western Cape. S Afr Med J 2006;96:538-543.
3. Strode A, Slack C, Mushariwa M: HIV vaccine research - south Africa's ethical-legal framework and its ability to promote the welfare of trial participants. S Afr Med J 2005;95:598-601.
4. Kagee A, Toefy Y, Simbayi L, Kalichman S: HIV prevalence in three predominantly Muslim residential areas in the Cape Town metropole. S Afr Med J 2005;95:512-516.
5. Lawn SD, Wood R: Tuberculosis control in South Africa - will HAART help? S Afr Med J 2006;96:502-504.
6. Soeters M, de Vries AM, Kimpen JL, Donald PR, Schaaf HS: Clinical features and outcome in children admitted to a TB hospital in the Western Cape - the influence of HIV infection and drug resistance. S Afr Med J 2005;95:602-606.
7. Fauci AS: Twenty-five years of HIV/AIDS. Science 2006;313:409.
8. Jaspan HB, Gray GE, Robinson AK, Coovadia HM, Bekker LG: Scientific justification for the participation of children and adolescents in HIV-1 vaccine trials in South Africa. S Afr Med J 2005;95:685-687.
9. Newburg DS: Human milk glycoconjugates that inhibit pathogens. Curr Med Chem 1999;6:117-127.
10. Naarding MA, Ludwig IS, Groot F, Berkhout B, Geijtenbeek TB, Pollakis G, Paxton WA: Lewis X component in human milk binds DC-SIGN and inhibits HIV-1 transfer to CD4+ T lymphocytes. J Clin Invest 2005;115: 3256-3264.
11. Yolken RH, Peterson JA, Vonderfecht SL, Fouts ET, Midthun K, Newburg DS: Human milk inhibits rotavirus replication and prevents experimental gastroenteritis. J Clin Invest 1992;90:1984-1991.
12. Nagashunmugam T, Friedman HM, Davis C, Kennedy S, Goldstein LT, Malamud D: Human submandibular saliva specifically inhibits HIV type 1. AIDS Res Hum Retroviruses 1997;13:371-376.
13. Bosch JA, De Geus EJ, Ligtenberg TJ, Nazmi K, Veerman EC, Hoogstraten J, Amerongen AV: Salivary MUC5B-mediated adherence (ex vivo) of Helicobacter pylori during acute stress. Psychosom Med 2000;62:40-49.
14. Situ H, Wei G, Smith CJ, Mashhoon S, Bobek LA: Human salivary MUC7 mucin peptides: effect of size, charge and cysteine residues on antifungal activity. Biochem J 2003;375(Pt 1):175-182.
15. Habte HH, Mall AS, de Beer C, Lotz ZE, Kahn D: The role of crude human saliva and purified salivary MUC5B and MUC7 mucins in the inhibition of human immunodeficiency virus type 1 in an inhibition assay. Virol J 2006;3:99.
16. Lallos LB, Laal S, Hoxie JA, Zolla-Pazner S, Bandres JC: Exclusion of HIV coreceptors CXCR4, CCR5 and CCR3 from the HIV envelope. AIDS Res Hum Retroviruses 1999;15:895-897.
17. Nara PL, Hatch WC, Dunlop NM, Robey WG, Arthur LO, Gonda MA, Fischinger PJ: Simple, rapid, quantitative, syncytium-forming microassay for the detection of human immunodeficiency virus neutralizing antibody. AIDS Res Hum Retroviruses 1987;3:283-302.
18. Habte HH, Kotwal GJ, Lotz ZE, Tyler MG, Abrahams M, Rodriques J, Kahn D, Mall AS: Antiviral activity of purified human breast milk mucin. Neonatology 2007;92:96-104.
19. Schroten H, Hanisch FG, Plogmann R, Hacker J, Uhlenbruck G, Nobis-Bosch R, Wahn V: Inhibition of adhesion of S-fimbriated Escherichia coli to buccal epithelial cells by human milk fat globule membrane components: a novel aspect of the protective function of mucins in the nonimmunoglobulin fraction. Infect Immun 1992;60:2893-2899.
20. Creeth JM, Denborough MA: The use of equilibrium-density-gradient methods for the preparation and characterization of blood-group-specific glycoproteins. Biochem J 1970;117:879-891.
21. Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227:680-685.
22. Thornton DJ, Howard M, Devine PL, Sheehan JK: Methods for separation and deglycosylation of mucin subunits. Anal Biochem 1995;227:162-167.
23. Klapper DG: A new low-cost, fully automated amino acid analyzer; in Elzinga M (ed): Methods in Protein Sequence Analysis. Clifton, Humana Press, 1982, pp 509-517.
24. Cohen AS, Strydom DJ: Amino acid analysis utilizing phenylisothiocyanate derivatives. Anal Biochem 1988;174:1-16.
25. Mantle M, Allen A: A colorimetric assay for glycoproteins based on the periodic acid/Schiff stain [proceedings]. Biochem Soc Trans 1978;6:607-609.
26. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193:265-275.
27. Carlstedt I, Sheehan J, Ulmsten U, Wingerup L: Isolation and purification of the mucin component of human cervical mucus. Adv Exp Med Biol 1982;144:273-275.
28. Mall AS, McLeod HA, Hickman R, Kahn D, Dent DM: Fragmentation pattern of mucins in normal and diseased gastric mucosae: a glycoprotein fractionates with gastric mucins purified from mucosal scrapings of cancer and peptic ulcer patients. Digestion 1999;60:216-226.
29. Patton S:Some practical implications of the milk mucins. J Dairy Sci 1999;82:1115-1117.
30. Patton S, Gendler SJ, Spicer AP: The epithelial mucin, MUC1, of milk, mammary gland and other tissues. Biochim Biophys Acta 1995;1241:407-423.
31. Peterson JA, Patton S, Hamosh M: Glycoproteins of human milk fat globule in the protection of the breast-fed infant against infections. Biol Neonate 1998;74:143-162.
32. Imam A, Laurence DJ, Neville AM: Isolation and characterization of two individual glycoprotein components from human milkfat-globule membranes. Biochem J 1982;207:37-41.
33. Imam A, Laurence DJ, Neville AM: Isolation and characterization of a major glycoprotein from milk-fat-globule membrane of human breast milk. Biochem J 1981;193:47-54.
34. Wiederschain GY, Newburg DS: Glycoconjugate stability in human milk: glycosidase activities and sugar release. J Nutr Biochem 2001;12:559-564.
35. Archibald DW, Cole GA: In vitro inhibition of HIV-1 infectivity by human salivas. AIDS Res Hum Retroviruses 1990;6:1425-1432.
36. Bergey EJ, Cho MI, Blumberg BM, Hammarskjöld ML, Rekosh D, Epstein LG, Levine MJ: Interaction of HIV-1 and human salivary mucins. J Acquir Immune Defic Syndr 1994;7:995-1002.
37. Shugars DC, Alexander AL, Fu K, Freel SA: Endogenous salivary inhibitors of human immunodeficiency virus. Arch Oral Biol 1999;44:445-453.
38. Shugars DC, Wahl SM: The role of the oral environment in HIV-1 transmission. J Am Dent Assoc 1998;129:851-858.
39. Thiry L, Sprecher-Goldberger S, Jonckheer T, Levy J, van de Perre P, Henrivaux P, Cogniaux-Leclerc J, Clumeck N: Isolation of AIDS virus from cell-free breast milk of three healthy virus carriers. Lancet 1985;2:891-892.
40. Lepage P, van de Perre P, Carael M, Nsengumuremyi F, Nkurunziza J, Butzler JP, Sprecher S: Postnatal transmission of HIV from mother to child. Lancet 1987;2:400.
41. van de Perre P, Simonon A, Hitimana DG, Dabis F, Msellati P, Mukamabano B, Butera J, van Goethem C, Karita E, Lepage P: Infective and anti-infective properties of breast milk from HIV-1-infected women. Lancet 1993;341:914-918.
42. Pallesen LT, Andersen MH, Nielsen RL, Berglund L, Petersen TE, Rasmussen LK, Rasmussen JT: Purification of MUC1 from bovine milk-fat globules and characterization of a corresponding full-length cDNA clone. J Dairy Sci 2001;84:2591-2598.
43. Baron S, Poast J, Cloyd MW: Why is HIV rarely transmitted by oral secretions? Saliva can disrupt orally shed, infected leukocytes. Arch Intern Med 1990;159:303-310.