Isolation and characterization of a serine proteinase with thrombin-like activity from the venom of the snake Bothrops asper

Brazilian Journal of Medical and Biological Research

Volumn 41, Issue 1, 2008, Pages 12-17

  Perez A V ^a,b   Rucavado, A ^a   Sanz, L ^c   Calvete, J J ^c   Gutierrez J M ^a  

^a UNIVERSIDAD DE COSTA RICA   (Costa Rica)

^b Univ Nac Autonoma de El Salvador   (El Salvador)

^c INSTITUTO DE BIOMEDICINA DE VALENCIA   (Spain)

Author keywords

Bothrops asper; Defibrin(ogen)ation; Serine proteinase; Snake venom; Thrombin like serine proteinase

Indexed keywords

FIBRINOGEN; SERINE PROTEINASE; SNAKE VENOM;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANIMAL EXPERIMENT; ARTICLE; BLOOD CLOTTING; BOTHROPS ASPER; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ISOLATION; FIBRINOLYSIS; HUMAN; IN VITRO STUDY; IN VIVO STUDY; ION EXCHANGE CHROMATOGRAPHY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; SEQUENCE HOMOLOGY; SNAKE;

BOTHROPS ASPER; CROTALINAE; MUS;

EID: 41149167528     PISSN: 0100879X     EISSN: 1414431X     Source Type: Journal    
DOI: 10.1590/S0100-879X2006005000189     Document Type: Article

References (20)

1. Fan HW, Cardoso JL. Clinical toxicology of snake bites in South America. In: Meier J, White J (Editors), Handbook of clinical toxicology of animal venoms and poisons. Boca Raton: CRC Press; 1995. p 667-688.
2. Gutiérrez JM. Clinical toxicology of snakebite in Central America. In: Meier J, White J (Editors), Handbook of clinical toxicology of animal venoms and poisons. Boca Raton: CRb Press; 1995. p 645-665.
3. Gutiérrez JM, Lomonte B. Efectos locales an el envenenamiento ofidico en América Latina. In: Cardoso JLC, Franga FOS, Wen FH, Málaque CMS, Haddad V (Editors), Animals peçonhentos no Brasil. Biologia, cithica e terapêutica dos acidentes. São Paulo: Sarvier; 2003. p 310-323.
4. Bolaños R. Las serpientes venenosas de Centro amrica y el problema del ofidismo. Primera parte. Aspectos zoológicos, epidemiológicos y biomédicos. Rev Costarric Cienc Med 1982; 3: 165-184.
5. Warrell DA. Epidemiology, clinical features and management of snake bites in Central and South America. In: Campbell JA, Lamar WW (Editors), Venomous reptiles of the Western hemisphere. Vol. 2. Ithaca: Cornell University Press; 2004. p 709-761.
6. Barrantes A, Solis V, Bolaños R. Alterations in the coagulation mechanisms of patients bitten by Bothrops asper (Terciopelo). Taxicon 1985; 23: 399-407.
7. Rucavado A, Soto M, Escalante T, Loña GD, Ami R, Gutiérrez JM. Thrombocytopenia and platelet hypoaggregation induced by Bothrops asper snake venom. Toxins involved and their contribution to metalloproteinase-induced pulmonary hemorrhage, Thromb Haemost 2005; 94: 123-131.
8. Gutiérrez JM, Rucavado A, Escalante T, Diaz C. Hemorrhage induced by snake venom metalloproteinases: biochemical and biophysical mechanisms involved in microvessel damage. Toxicon 2005; 45: 997-1011.
9. White J. Snake venoms and coagulopathy. Toxicon 2005; 45: 951-967.
10. Markland FS. Snake venoms and the hemostatic system. Toxicon 1998; 36: 1749-1800.
11. Serrano SM, Maroun RC. Snake venom serine proteinases: sequence, homology vs substrate specificity, a paradox to be solved. Toxicon 2005; 45: 1115-1132.
12. Loría GD, Rucavado A, Kamiguti AS, Theakston RD, Fox JW, Alape A, et al. Characterization of 'basparin A', a prothrombin-activating metalloproteinase, from the venom of the snake Bothrops asper that inhibits platelet aggregation and induces defibrination and thrombosis. Arch Biochem Biophys 2003; 418: 13-24.
13. Ortiz FA, Gubensek F. Isolation and some properties of blood clotting enzyme from the venom of Bothrops asper. Bull Inst Pasteur 1976; 74: 145-148.
14. Aragón F, Gubensek F. Characterization of thrombin-like proteinase from Bothrops asper venom. In: Rosenberg P (Editor), Toxins: animal, plant and microbial. Oxford: Pergamon Press; 1978. p 107-111.
15. Rucavado A, Escalante T, Gutiérrez JM. Effect of the metalloproteinase inhibitor batimastat in the systemic toxicity induced by Bothrops asper snake venom: understanding the role of metalloproteinases in envenomation. Toxicon 2004; 43: 417-424.
16. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680-685.
17. Le Blanc JC, Hager JW, Ilisiu AM, Hunter C, Zhong F, Chu i. Unique scanning capabilities of a new hybrid linear ion trap mass spectrometer (Q TRAP) used for high sensitivity proteomics applications. Proteomics 2003; 3: 859-869.
18. Theakston RD, Reid HA. Development of simple standard assay procedures for the characterization of snake venom. Bull World Health Organ 1983; 61: 949-956.
19. Sant'Ana CD, Ticli FK, Oliveira LL, Giglio JR, Rechia CG, Fuly AL, et al. BjussuSP-1: A new thrombin-like enzyme isolated from Bothrops jararacussu snake venom. Comp Biochem Physiol A Mol Integr Physiol 2007 (in press).
20. Alexander G, Grothusen J, Zepeda H, Schwartzman RJ. Gyroxin, a toxin from the venom of Crotalus durissus terrificus, is a thrombin-like enzyme. Toxicon 1988; 26: 953-960.