Emodin accelerates osteoblast differentiation through phosphatidylinositol 3-kinase activation and bone morphogenetic protein-2 gene expression

International Immunopharmacology

Volumn 8, Issue 5, 2008, Pages 741-747

  Lee, Su Ui ^a,b   Shin, Hye Kyoung ^b   Min, Yong Ki ^b   Kim, Seong Hwan ^b  

^a Chungnam National University   (South Korea)

^b Korea Research Institute of Chemical Technology   (South Korea)

Author keywords

Bone morphogenetic protein 2; Emodin; Osteoblast differentiation; Phosphatidylinositol 3 kinase

Indexed keywords

2 MORPHOLINO 8 PHENYLCHROMONE; ALKALINE PHOSPHATASE; BONE MORPHOGENETIC PROTEIN 2; EMODIN; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; MITOGEN ACTIVATED PROTEIN KINASE; PHOSPHATIDYLINOSITOL 3 KINASE; PROTEIN KINASE B;

ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; CELL GROWTH; CONTROLLED STUDY; ENZYME ACTIVATION; GENE EXPRESSION; MINERALIZATION; MOUSE; NONHUMAN; OSTEOBLAST; OSTEOPOROSIS; PRIORITY JOURNAL;

1-PHOSPHATIDYLINOSITOL 3-KINASE; 3T3 CELLS; ALKALINE PHOSPHATASE; ANIMALS; ANTHRAQUINONES; BLOTTING, WESTERN; BONE MORPHOGENETIC PROTEIN RECEPTORS, TYPE II; CELL DIFFERENTIATION; CHROMONES; CLONING, MOLECULAR; COLORING AGENTS; EMODIN; ENZYME ACTIVATION; ENZYME INHIBITORS; GENES, REPORTER; LUCIFERASES; MICE; MORPHOLINES; OSTEOBLASTS; RNA, MESSENGER; TRANSFECTION;

EID: 41149150641     PISSN: 15675769     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.intimp.2008.01.027     Document Type: Article

References (38)

1. Harada S., and Rodan G.A. Control of osteoblast function and regulation of bone mass. Nature 423 (2003) 349-355
2. van der Klift M., de Laet C.D., and Pols H.A. Assessment of fracture risk: who should be treated for osteoporosis?. Best Pract Res Clin Rheumatol 19 (2005) 937-950
3. Rodan G.A., and Martin T.J. Therapeutic approaches to bone diseases. Science 289 (2000) 1508-1514
4. Zhao G.Q. Consequences of knocking out BMP signaling in the mouse. Genesis 35 (2003) 43-56
5. Cheng H., Jiang W., Phillips F.M., Haydon R.C., Peng Y., Zhou L., et al. Osteogenic activity of the fourteen types of human bone morphogenetic proteins (BMPs). J Bone Jt Surg Am 85A (2003) 1544-1552
6. Kang Q., Sun M.H., Cheng H., Peng Y., Montag A.G., Deyrup A.T., et al. Characterization of the distinct orthotopic bone-forming activity of 14 BMPs using recombinant adenovirus-mediated gene delivery. Gene Ther 11 (2004) 1312-1320
7. Mundy G., Garrett R., Harris S., Chan J., Chen D., Rossini G., et al. Stimulation of bone formation in vitro and in rodents by statins. Science 286 (1999) 1946-1949
8. Wang D., Christensen K., Chawla K., Xiao G., Krebsbach P.H., and Franceschi R.T. Isolation and characterization of MC3T3-E1 preosteoblast subclones with distinct in vitro and in vivo differentiation/mineralization potential. J Bone Miner Res 14 (1999) 893-903
9. Ghosh-Choudhury N., Choudhury G.G., Harris M.A., Wozney J., Mundy G.R., Abboud S.L., et al. Autoregulation of mouse BMP-2 gene transcription is directed by the proximal promoter element. Biochem Biophys Res Commun 286 (2001) 101-108
10. Rozen S., and Skaletsky H.J. Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S., and Misener S. (Eds). Bioinformatics Methods and Protocols: Methods in Molecular Biology (2000), Humana Press, Totowa 365-386
11. - ΔΔCT method. Methods 25 (2001) 402-408
12. Ghosh-Choudhury N., Mandal C.C., and Choudhury G.G. Statin-induced Ras activation integrates the phosphatidylinositol 3-kinase signal to Akt and MAPK for bone morphogenetic protein-2 expression in osteoblast differentiation. J Biol Chem 282 (2007) 4983-4993
13. Putnam S.E., Scutt A.M., Bicknell K., Priestley C.M., and Williamson E.M. Natural products as alternative treatments for metabolic bone disorders and for maintenance of bone health. Phytother Res 21 (2007) 99-112
14. Chen K.M., Ge B.F., Ma H.P., Liu X.Y., Bai M.H., and Wang Y. Icariin, a flavonoid from the herb Epimedium enhances the osteogenic differentiation of rat primary bone marrow stromal cells. Pharmazie 60 (2005) 939-942
15. Yamaguchi M., and Gao Y.H. Anabolic effect of genistein and genistin on bone metabolism in the femoral-metaphyseal tissues of elderly rats: the genistein effect is enhanced by zinc. Mol Cell Biochem 178 (1998) 377-382
16. Sugimoto E., and Yamaguchi M. Anabolic effect of genistein in osteoblastic MC3T3-E1 cells. Int J Mol Med 5 (2000) 515-520
17. Yamaguchi M., Sugimoto E., and Hachiya S. Stimulatory effect of menaquinone-7 (vitamin K2) on osteoblastic bone formation in vitro. Mol Cell Biochem 223 (2001) 131-137
18. Pan W., Quarles L.D., Song L.H., Yu Y.H., Jiao C., Tang H.B., et al. Genistein stimulates the osteoblastic differentiation via NO/cGMP in bone marrow culture. J Cell Biochem 94 (2005) 307-316
19. Vali B., Rao L.G., and El-Sohemy A. Epigallocatechin-3-gallate increases the formation of mineralized bone nodules by human osteoblast-like cells. J Nutr Biochem 18 (2007) 341-347
20. Mizutani K., Ikeda K., Kawai Y., and Yamori Y. Resveratrol stimulates the proliferation and differentiation of osteoblastic MC3T3-E1 cells. Biochem Biophys Res Commun 253 (1998) 859-863
21. Wang H.H., and Chung J.G. Emodin-induced inhibition of growth and DNA damage in the Helicobacter pylori. Curr Microbiol 35 (1997) 262-266
22. Chang C.H., Lin C.C., Yang J.J., Namba T., and Hattori M. Anti-inflammatory effects of emodin from Ventilago leiocarpa. Am J Chin Med 24 (1996) 139-142
23. Huang H.C., Chang J.H., Tung S.F., Wu R.T., Foegh M.L., and Chu S.H. Immunosuppressive effect of emodin, a free radical generator. Eur J Pharmacol 211 (1992) 359-364
24. Sato M., Maulik G., Bagchi D., and Das D.K. Myocardial protection by protykin, a novel extract of trans-resveratrol and emodin. Free Radic Res 32 (2000) 135-144
25. Goel R.K., Das Gupta G., Ram S.N., and Pandey V.B. Antiulcerogenic and anti-inflammatory effects of emodin, isolated from Rhamnus triquerta wall. Indian J Exp Biol 29 (1991) 230-232
26. Jing X., Ueki N., Cheng J., Imanishi H., and Hada T. Induction of apoptosis in hepatocellular carcinoma cell lines by emodin. Jpn J Cancer Res 93 (2002) 874-882
27. Savarino L., Benetti D., Baldini N., Tarabusi C., Greco M., Aloisi R., et al. A preliminary in vitro and in vivo study of the effects of new anthraquinones on neutrophils and bone remodeling. J Biomed Mater Res A 75 (2005) 324-332
28. Feng J.Q., Xing L., Zhang J.H., Zhao M., Horn D., Chan J., et al. NF-κB specifically activates BMP-2 gene expression in growth plate chondrocytes in vivo and in a chondrocyte cell line in vitro. J Biol Chem 278 (2003) 29130-29135
29. Hipskind R.A., and Bilbe G. MAP kinase signaling cascades and gene expression in osteoblasts. Front Biosci 3 (1998) d804-d816
30. Suzuki A., Guicheux J., Palmer G., Miura Y., Oiso Y., Bonjour J.P., et al. Evidence for a role of p38 MAP kinase in expression of alkaline phosphatase during osteoblastic cell differentiation. Bone 30 (2002) 91-98
31. Whitmarsh A.J., and Davis R.J. Transcription factor AP-1 regulation by mitogen-activated protein kinase signal transduction pathways. J Mol Med 74 (1996) 589-607
32. McCabe L.R., Kockx M., Lian J., Stein J., and Stein G. Selective expression of fos- and jun-related genes during osteoblast proliferation and differentiation. Exp Cell Res 218 (1995) 255-262
33. Stein G.S., Lian J.B., Stein J.L., Van Wijnen A.J., and Montecino M. Transcriptional control of osteoblast growth and differentiation. Physiol Rev 76 (1996) 593-629
34. Xu J., and Rogers M.B. Modulation of bone morphogenetic protein (BMP) 2 gene expression by Sp1 transcription factors. Gene 392 (2007) 221-229
35. Wang C., Wu X., Chen M., Duan W., Sun L., Yan M., and Zhang L. Emodin induces apoptosis through caspase 3-dependent pathway in HK-2 cells. Toxicology 231 (2007) 120-128
36. Guo J.M., Xiao B.X., Liu Q., Zhang S., Liu D.H., and Gong Z.H. Anticancer effect of aloe-emodin on cervical cancer cells involves G2/M arrest and induction of differentiation. Acta Pharmacol Sinica 28 (2007) 1991-1995
37. Guo J., Xiao B., Zhang S., Liu D., Liao Y., and Sun Q. Growth inhibitory effects of gastric cancer cells with an increase in S phase and alkaline phosphatase activity repression by aloe-emodin. Cancer Biol Ther 6 (2007) 85-88
38. Chen H.C., Hsieh W.T., Chang W.C., and Chung J.G. Aloe-emodin induced in vitro G2/M arrest of cell cycle in human promyelocytic leukemia HL-60 cells. Food Chem Toxicol 42 (2004) 1251-1257