Effect of testosterone, estrone sulphate and androstenone on 3β-hydroxysteroid dehydrogenase protein expression in primary cultured hepatocytes

Livestock Science

Volumn 114, Issue 2-3, 2008, Pages 202-210

  Nicolau Solano, S I ^a   Doran, O ^a  

^a UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

3 Hydroxysteroid dehydrogenase; Boar taint; Hepatocytes; Pig; Steroids

Indexed keywords

ANIMALIA; SUIDAE;

EID: 40749127061     PISSN: 18711413     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.livsci.2007.05.001     Document Type: Article

References (36)

1. Allrich A.D., Christenson R.K., Ford J.J., and Zimmerman D.R. Pubertal development of the boar: testosterone, estradiol-17 beta, cortisol and LH concentrations before and after castration at various ages. J. Anim. Sci. 55 (1982) 1139-1146
2. Andresen O. Concentrations of fat and plasma 5alpha-androstenone and plasma testosterone in boars selected for rate of body weight gain and thickness of back fat during growth, sexual maturation and after mating. J. Reprod. Fertil. 48 (1976) 51-59
3. Austin C.J., Emberson L., and Nicholls P. Purification and characterization of pheromaxein, the porcine steroid-binding protein-A member of the secretoglobin superfamily. Eur. J. Biochem. 271 (2004) 2593-2606
4. Babol J., Squires E.J., and Gullett E.R. Investigation of factors responsible for the development of boar taint. Food Res. Int. 28 (1995) 573-581
5. Babol J., Squires E.J., and Lundstrom K. Relationship between metabolism of androstenone and skatole in intact male pigs. J. Anim. Sci. 77 (1999) 84-92
6. Bonneau M. Compounds responsible for boar taint, with special emphasis on androstenone: a review. Livest. Prod. Sci. 9 (1982) 687-705
7. Booth W.D. Changes with age in the occurrence of C19 steroids in the testis and submaxillary gland of the boar. J. Reprod. Fertil. 42 (1975) 459-472
8. Bradford M.M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72 (1976) 248-254
9. Centol I., Lopez-Blanco F., Deniz A., Benitez L., Marrero-Arencibia I., Fanjul L., and De Galarreta S.M.R. Serum androgen levels after streptozotocin administration in the male rat. Rev. Esp. Fisiol. 44 (1988) 31-34
10. Christenson R.K., Ford J.J., and Redmer D.A. Estradiol and testosterone metabolic clearance and production rates during pubertal development in boars. Biol. Reprod. 31 (1984) 905-912
11. Claus R., Wagner A., and Lambert T. Characterization of 11β-hydroxysteroid dehydrogenase activity in testicular tissue of control and GnRH-immunized boars as a possible regulator of spermatogenesis. Exp. Clin. Endocrinol. Diabetes 113 (2005) 262-267
12. Davis S.M., and Squires E.J. Association of cytochrome b5 with 16-androstene steroid synthesis in the testis and accumulation in the fat of male pigs. J. Anim. Sci. 77 (1999) 1230-1235
13. De Galarreta S.M.R., Fanjul L.F., Meidan R., and Hsueh A.J. Regulation of 3 beta-hydroxysteroid dehydrogenase activity by human chorionic gonadotropin, androgens, and anti-androgens in cultured testicular cells. J. Biol. Chem. 258 (1983) 10988-10996
14. Doran E., Whittington F.M., Wood J.D., and McGivan J.D. Cytochrome 450IIE1 (CYP2E1) is induced by skatole and this induction is blocked by androstenone in isolated pig hepatocytes. Chem.-Biol. Interact. 140 (2002) 81-92
15. Doran E., Whittington F.M., Wood J.D., and McGivan J.D. Characterisation of androstenone metabolism in pig liver microsomes. Chem.-Biol. Interact. 147 (2004) 141-149
16. Forland D.M., Lundstrom K., and Andresen O. Relationship between androstenone content in fat, intensity of boar taint and size of accessory sex glands in boars. Nord. Vet.Med. 32 (1980) 201-206
17. Gallon C., Veyssiere G., Berger M., Jean-Faucher C., De Turckheim M., and Jean C. Age-related changes in the concentration of cytosolic androgen receptors in the epididymis, vas deferens and seminal vesicle of maturing male mice. J. Androl. 10 (1989) 188-194
18. Gower D.B. 16-Unsaturated C 19 steroids. A review of their chemistry, biochemistry and possible physiological role. J. Steroid Biochem. 3 (1972) 45-103
19. Heggland S.J., Signs S.A., and Stalvey J.R. Testosterone decreases 3beta-hydroxysteroid dehydrogenase-isomerase messenger ribonucleic acid in cultured mouse Leydig cells by a strain-specific mechanism. J. Androl. 18 (1997) 646-655
20. Lax E.R., Tamulevicius P., and Muller A. Hepatic nuclear estrogen receptor concentration in the rat - influence of age, sex, gestation, lactation and estrous cycle. J. Steroid Biochem. 19 (1983) 1083-1088
21. Louveau I., Bonneau M., and Gower D.B. Biosynthesis of 16-androstene steroids and testosterone by porcine testis tissue in vitro: effect of age and relationships with fat 5 alpha-androstenone levels in vivo. Acta Endocrinol. (Copenh) 125 (1991) 526-531
22. Lundstrom, K., Bonneau, M., 1996. Off-flavour in meat with particular emphasis on boar taint. In: Meat Quality and Meat Packaging (Ed. ECCEAMST), Utrecht, The Netherlands, 137-154.
23. Matsumoto A., and Prins G.S. Age-dependent changes in androgen receptor immunoreactivity in motoneurons of the spinal nucleus of the bulbocavernosus of male rats. Neurosci. Lett. 243 (1998) 29-32
24. McGinnis M., and Yu W.H.A. Age-related changes in androgen receptor levels in cranial nerve nuclei of male rats. Brain Res. Bull. 36 (1995) 581-585
25. Naville D., Keeney D.S., Jenkin G., Murry B.A., Head J.R., and Mason J.I. Regulation of expression of male-specific rat liver microsomal 3 beta-hydroxysteroid dehydrogenase. Mol. Endocrinol. 5 (1991) 1090-1100
26. Nicolau-Solano S.I., McGivan J.D., Whittington F.M., Nieuwhof G.I., Wood J.D., and Doran O. Relationship between the expressions of hepatic but not testicular 3β-hydroxysteroid dehydrogenase with androstenone deposition in pig adipose tissue. J. Anim. Sci. 84 (2006) 2809-2817
27. Patterson R.L. 5alpha-androst-16-en-3one compound responsible for taint in boar fat. J. Sci. Food Agric. 19 (1968) 31-38
28. Scientific Report of the Scientific Panel for Animal Health and Welfare on request from the Commission related to welfare aspects of the castration of piglets, 2004. Welfare aspect of castration of piglets. EFSA J, 91, 11/100.
29. Schwenk M., and El Pino V.L. Uptake of estrone sulphate by isolated rat liver cells. J. Steroid Biochem. 13 (1980) 669-673
30. Sinclair P.A., Hancock S., Gilmore W.J., and Squires E.J. Metabolism of the 16-androstene steroids in primary cultured porcine hepatocytes. J. Steroid Biochem. Mol. Biol. 96 (2005) 79-87
31. Soma K.K., Sinchak K., Lakhter A., Schlinger B.A., and Micevych P.E. Neurosteroids and female reproduction: estrogen increases 3β-HSD mRNA and activity in rat hypothalamus. Endocrinology 146 (2006) 4386-4390
32. Stalvey J.R., and Clavey S.M. Evidence that testosterone regulates Leydig cell 3 beta-hydroxysteroid dehydrogenase-isomerase activity by a trans-acting factor distal to the androgen receptor. J. Androl. 13 (1992) 93-99
33. Supakar P.C., and Roy A.K. Role of transcription factors in the age-dependent regulation of the androgen receptor gene in rat liver: review. Biol. Signals 5 (1996) 170-179
34. Urabe M., Chetrite G., and Pasqualini J.R. Transformation of estrone, estradiol, and estrone sulphate in uterine and vaginal isolated cells of fetal guinea pigs. Effect of various antiestrogens in the conversion of estrone sulphate to estradiol. Steroids 58 (1993) 209-214
35. Zamaratskaia G., Babol J., Madej A., Squires E.J., and Lundstrom K. Age-related variation of plasma concentrations of skatole, androstenone, testosterone, oestradiol-17β, oestrone sulphate, dehydroepiandrosterone sulphate, triiodothyronine and IGF-1 in six entire male pigs. Reprod. Domest. Anim. 39 (2004) 168-172
36. Zamaratskaia G., Babol J., Madej A., Squires E.J., and Lundstrom K. Free oestrone in adipose tissue and its relation to androstenone and skatole in entire male pigs. Reprod. Domest. Anim. 40 (2005) 156-160