Association of cytochrome b5 with 16-androstene steroid synthesis in the testis and accumulation in the fat of male pigs

Journal of Animal Science

Volumn 77, Issue 5, 1999, Pages 1230-1235

  Davis, S M ^a   Squires, E J ^a  

^a UNIVERSITY OF GUELPH   (Canada)

Author keywords

Androstenone; Boar Taint; Cytochrome b5; Cytochrome P450; Steroids

Indexed keywords

SUS SCROFA;

16-ANDROSTENE; ANDROST 16 ENE; ANDROSTA 5,16 DIEN 3 BETA OL SYNTHETASE; ANDROSTA-5,16-DIEN-3 BETA-OL SYNTHETASE; ANDROSTANE DERIVATIVE; CYTOCHROME B5; OXIDOREDUCTASE; STEROID 17ALPHA MONOOXYGENASE;

ADIPOSE TISSUE; ANIMAL; ANIMAL DISEASE; ARTICLE; MALE; METABOLISM; MOLECULAR WEIGHT; SWINE; TESTIS; WESTERN BLOTTING;

ADIPOSE TISSUE; ANDROSTENES; ANIMALS; BLOTTING, WESTERN; CYTOCHROMES B5; MALE; MOLECULAR WEIGHT; OXIDOREDUCTASES; STEROID 17-ALPHA-HYDROXYLASE; SWINE; TESTIS;

EID: 0033126759     PISSN: 00218812     EISSN: None     Source Type: Journal    
DOI: 10.2527/1999.7751230x     Document Type: Article

References (26)

1. 5. J. Biochem. 97:1659-1668.
2. Babol, J., and E. J. Squires. 1995. Quality of meat from entire male pigs. Food Res. Int. 28:201-212.
3. Babol, J., E. J. Squires, and K. Lundström. 1998a. Hepatic metabolism of skatole in pigs by cytochrome P4502E1. J. Anim. Sci. 76:822-828.
4. Babol, J., E. J. Squires, and K. Lundström. 1998b. Relationship between oxidation and conjugation metabolism of skatole in pig liver and concentrations of skatole in fat. J. Anim. Sci. 76: 829-838.
5. Betz, G., and P. Tsai. 1978. Stimulatory effect of soluble supernatant on hydroxylase activity of rat testis microsomes. Steroids 32: 389-397.
6. Bonneau, M., J. Carrié-Lemoine, A. Prunier, D. H. Garnier, and M. Terqui. 1987. Age-related changes in plasma LH and testosterone concentration profiles and fat 5α-androstenone content in the young boar. Anim. Reprod. Sci. 15:241-258.
7. Bonneau, M., W. J. Meadus, and E. J. Squires. 1992. Effects of exogenous porcine somatotropin on performance, testicular steroid production and fat levels of boar-taint-related compounds in young boars. Can. J. Anim. Sci. 72:537-545.
8. Bonneau, M., N. Meusy-Dessolle, P. C. Léglise, and R. Claus. 1982a. Relationships between fat and plasma androstenone and plasma testosterone in fatty and lean young boars following castration. Acta Endocrinol. 101:129-133.
9. Bonneau, M., N. Meusy-Dessolle, P. C. Léglise, and R. Claus. 1982b. Relationships between fat and plasma androstenone and testosterone in fatty and lean young boars during growth and after hCG stimulation. Acta Endocrinol. 101:119-128.
10. 5 gene, and a transcribed pseudogene. Genomics 17:348-354.
11. 5 gene from a patient with congenital methemoglobinemia and pseudohermaphrodism. Human Genetics 93:568-570.
12. 5 from human erythrocytes. Biochem. Biophys. Res. Commun. 60:28-34.
13. Katkov, T., and D. B. Gower. 1970. The biosynthesis of androst-16-enes in boar testis tissue. Biochem. J. 117:533-538.
14. 5. FEES Lett. 169: 143-146.
15. Kwano, T., Y. Kozutsumi, T. Kawasaki, and A. Suzuki. 1994. Biosynthesis of N-glycolylneuraminic acid-containing glycoconjugates. J. Biol. Chem. 269:9024-9029.
16. Lee-Robichaud, P., J. N. Wright, M. E. Akhtar, and M. Akhtar. 1995. Modulation of the activity of human 17α-hydroxylase-17,20-lyase (CYP17) by cytochrome b5: Endocrinological and mechanistic implications. Biochem. J. 308:901-908.
17. Meadus, W. J., J. I. Mason, and E. J. Squires. 1993. Cytochrome P450c17 from porcine and bovine adrenal catalyses the formation of 5,16-androstadien-3β-ol from pregnenolone in the presence of cytochrome b5. J. Steroid Biochem. Mol. Biol. 46: 565-572.
18. Nakajin, S., K. Takahashi, M. Shinoda, and P. F. Hall. 1985. Cytochrome b5 promotes the synthesis of delta-16 C19 steroids by homogeneous cytochrome P-450 C21 side-chain cleavage from pig testis. Biochem. Biophys. Res. Commun. 132:708-713.
19. Patterson, R. S. 1968. 5α-androst-16-en-3-one: Compound responsible for boar taint in the boar. J. Sci. Food Agric. 19:31-37.
20. Sakai, Y., T. Yanase, R. Takayanagi, R. Nako, Y. Nishi, M. Haji, and H. Nawata. 1993. High expression of cytochrome b5 in adrenocortical adenomas from patients with Cushings syndrome associated with high secretion of adrenal androgens. J. Clin. Endocrinol. Metab. 76:1286-1290.
21. SAS. 1991. SAS/STAT® User's Guide. SAS Inst. Inc., Cary, NC.
22. Squires, E. J. 1990. Studies on the suitability of a colorimetric test for androst-16-ene steroids in the submaxillary gland and fat of pigs as a simple chemical test for boar taint. Can. J. Anim. Sci. 70:1029-1040.
23. Squires, E. J., O. Adeola, L. G. Young, and R. R. Hacker. 1993a. The role of growth hormones, β-adrenergic agents and intact males in pork production: A review. Can. J. Anim. Sci. 73:1-23.
24. Squires, E. J., H. Deng, and L. Wu. 1993b. Colorimetric method for the determination of androst-16-ene steroids in carcasses. In: M. Bonneau (Ed.) Measurement and Prevention of Boar Taint in Entire Male Pigs. pp 41-48. INRA Editions, Paris.
25. Squires, E. J., and K. Lundström. 1997. Relationship between cytochrome P450IIE1 in liver and levels of skatole and its metabolites in intact male pigs. J. Anim. Sci. 75:2506-2511.
26. Xue, J., G. D. Dial, E. E. Holton, Z. Vickers, E. J. Squires, Y. Lou, D. Godbout, and N. Morel. 1996. Breed differences in boar taint: Relationship between tissue levels of boar taint compounds and sensory analysis of taint. J. Anim. Sci. 74:2170-2177.