Substrate desolvation as a governing factor in enzymatic transformations of PAHs in aqueous-acetonitrile mixtures

Biotechnology Progress

Volumn 20, Issue 4, 2004, Pages 1251-1254

  Borole, Abhijeet P ^a   Cheng, Catherine L ^b,c   Davison, Brian H ^a  

^a OAK RIDGE NATIONAL LABORATORY   (United States)

^b UNIVERSITY OF TENNESSEE   (United States)

^c ELI LILLY AND COMPANY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACETONITRILE; ACTIVATION ENERGY; ENZYME KINETICS; HYDROPHOBICITY; MIXTURES; PROTEINS; REACTION KINETICS; SOLUTIONS; SOLVENTS; SUBSTRATES;

AQUEOUS-ORGANIC MEDIA; ENZYMATIC TRANSFORMATION; HYDROPHOBIC SUBSTRATES;

BIOTECHNOLOGY;

ACETONITRILE; ACETONITRILE DERIVATIVE; CHYMOTRYPSIN; POLYCYCLIC HYDROCARBON; SUBTILISIN; WATER;

ARTICLE; BIOTRANSFORMATION; CHEMISTRY; ENZYME SPECIFICITY; METABOLISM; SOLUBILITY; THERMODYNAMICS;

ACETONITRILES; BIOTRANSFORMATION; CHYMOTRYPSIN; POLYCYCLIC COMPOUNDS; SOLUBILITY; SUBSTRATE SPECIFICITY; SUBTILISINS; THERMODYNAMICS; WATER;

EID: 4043168308     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp034302r     Document Type: Article

References (14)

1. Mogollon, L.; Rodriguez, R.; Larrota, W.; Ortiz, C.; Torres, R. Biocatalytic removal of nickel and vanadium from petroporphyrins and asphaltenes. Appl. Biochem. Biotechnol. 1998, 70-72, 765-777.
2. Fedorak, P. M.; Semple, K. M.; Vazquez-Duhalt, R.; Westlake, D. W. S. Chloroperoxidase-mediated modifications of petroporphyrins and asphaltenes. Enzyme Microb. Technol. 1993, 15, 429-437.
3. Vazquez-Duhalt, R.; Westlade, D. W. S.; Fedorak, P. M. U. A. Lignin peroxidase oxidation of aromatic compounds in systems containing organic solvents. Appl. Environ. Microbiol. 1994, 60, 459-466.
4. Monticello, D. J. Riding the fossil fuel biodesulfurization wave. CHEMTECH 1998, July, 38-45.
5. Torres, E.; Tinoco, R.; Vazquez-Duhalt, R. Biocatalytic oxidation of polycyclic aromatic hydrocarbons in media containing organic solvents. Water Sci. Technol. 1997, 36, 37-44.
6. Torres, E.; Tinoco, R.; Vazquez-Duhalt, R. Solvent hydrophobicity predicts biocatalytic behavior of lignin peroxidase and cytochrome c in aqueous solutions of water-miscible organic solvents. J. Biotechnol. 1996, 49, 59-67.
7. Torres, E.; Siminovich, B.; Barzana, E.; Vazquez-Duhalt, R. Thermodynamic hydrophobicity of aqueous-miscible organic solvents predicts peroxidase activity. J. Mol. Catal. B: Enzym. 1998, 4, 155-159.
8. Klibanov, A. M. Why are enzymes less active in organic solvents than in water? Trends Biotechnol. 1997, 15, 97-101.
9. Wangikar, P. P.; Rich, J. O.; Clark, D. S.; Dordick, J. S. Probing enzymatic transition-state hydrophobicities. Biochemistry 1995, 34, 12302-12310.
10. Wescott, C. R.; Klibanov, A. M. Thermodynamic analysis of solvent effect on substrate specificity of lyophilized enzymes suspended in organic media. Biotechnol. Bioeng. 1997, 56, 340-344.
11. Kim, J.; Clark, D. S.; Dordick, J. S. Intrinsic effects of solvent polarity on enzymic activation energies. Biotechnol. Bioeng. 2000, 67, 112-116.
12. Dowd, J. E.; Riggs, D. S. A comparison of estimates of Michaelis-Menten kinetic constants from various linear transformations. J. Biol. Chem. 1965, 240, 863-869.
13. Vazquez-Duhalt, R.; Semple, K. M.; Westlate, D. W. S.; Fedorak, P. M. Effect of water-miscible organic solvents on the catalytic activity of cytochrome c. Enzyme Microb. Technol. 1993, 15, 936-943.
14. Fersht, A. Structure and Mechanism in Protein Science; W. H. Freeman and Company: New York, 1999; p 631.