Evaluation of disruption methods for the release of intracellular recombinant protein from Escherichia coli for analytical purposes

Biotechnology and Applied Biochemistry

Volumn 40, Issue 1, 2004, Pages 83-88

  Tkac, Jan ^a,b   Vostiar, Igor ^a,c   Mandenius, Carl Fredrik ^a  

^a LINKÖPING UNIVERSITY   (Sweden)

^b INSTITUTE OF CHEMISTRY   (Slovakia)

^c SLOVAK UNIVERSITY OF TECHNOLOGY   (Slovakia)

Author keywords

Bioreactor monitoring; Cell disruption; Fermentation analysis; Recombinant human superoxide dismutase assay (rhSOD assay); Surface plasmon resonance (SPR)

Indexed keywords

BIOSENSORS; CELLS; ESCHERICHIA COLI; OSMOSIS; SPECTROPHOTOMETERS; SURFACE ACTIVE AGENTS;

CELL-CULTURE; COLORIMETRIC REAGENT; DISRUPTION METHODS;

PROTEINS;

NONIONIC SURFACTANT; RECOMBINANT ENZYME; RECOMBINANT PROTEIN; SUPEROXIDE DISMUTASE;

ARTICLE; BIOSENSOR; CELL DISRUPTION; COLORIMETRY; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME BINDING; ESCHERICHIA COLI; INTERMETHOD COMPARISON; NONHUMAN; OSMOTIC STRESS; PROTEIN SECRETION; REPRODUCIBILITY; SPECIES CULTIVATION; SPECTROPHOTOMETER; SURFACE PLASMON RESONANCE; ULTRASOUND;

BACTERIOLYSIS; CELL FRACTIONATION; ENZYME ACTIVATION; ESCHERICHIA COLI; HUMANS; OSMOTIC PRESSURE; RECOMBINANT PROTEINS; SONICATION; SUPEROXIDE DISMUTASE; SURFACE-ACTIVE AGENTS;

ESCHERICHIA COLI;

EID: 4043133078     PISSN: 08854513     EISSN: None     Source Type: Journal    
DOI: 10.1042/BA20030143     Document Type: Article

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