Flow injection analysis of intracellular β-galactosidase in Escherichia coli cultivations, using an on-line system including cell disruption, debris separation and immunochemical quantification

Bioseparation

Volumn 8, Issue 1-5, 1999, Pages 255-267

  Tocaj, Anita ^a   Nandakumar, M P ^a   Holst, O ^a   Mattiasson, B ^a  

^a LUND UNIVERSITY   (Sweden)

Author keywords

Beta galactosidase; Immunoassay; Micro expanded bed; On line disintegration

Indexed keywords

ASSAY; BETA GALACTOSIDASE; CELL DISRUPTION; CELL SEPARATION; ESCHERICHIA COLI; EXPANDED BED ADSORPTION CHROMATOGRAPHY; FLOW INJECTION METHOD; IMMOBILIZED ENZYME; LYSOZYME; ONLINE MONITORING;

BIOASSAY; CELLS; ENZYME IMMOBILIZATION; ESCHERICHIA COLI; ULTRASONIC APPLICATIONS;

CELL DISRUPTION; FLOW INJECTION ANALYSIS; GALACTOSIDASE;

FERMENTATION;

ESCHERICHIA COLI;

EID: 0033375073     PISSN: 0923179X     EISSN: None     Source Type: Journal    
DOI: 10.1007/978-94-017-1519-5_28     Document Type: Article

References (28)

1. Agerkvist I and Enfors S-O (1990). Characterisation of E. coli cell disintegrates from a bead mill and high pressure homogenizers. Biotechnol. Bioeng. 36: 1083-1089.
2. Ahlmann N, Niehoff A, Rinas U, Scheper T and Schügerl K (1986). Continuous on-line monitoring of intracellular enzyme activity. Anal. Chim. Acta 190: 221-226.
3. Andrews BA and Asenjo JA (1987). Enzymatic lysis and disruption of microbial cells. Trends. Biotechnol 5: 273-277.
4. Blankenstein G and Kula M-R (1991). Cell permeabilization as a tool for measurement of intracellular enzyme activity in a flow-injection system. Anal. Chim. Acta 248: 371-378.
5. Borrebaeck C, Börjesson J and Mattiasson B (1978). Thermometric enzyme linked immunosorbent assay in continuous flow systems: Optimization and evaluation using human serum albumin as a modelsystem. Clin. Chim. Acta 86: 267-278.
6. Brock TD and Madigan MT (1988). Biology of microorganisms. Prentice-Hall International, Englewood Cliffs, New Jersey.
7. Dean CR and Ward OP (1992). The use of EDTA or polymyxin with lysozyme for the recovery of intracellular products from Escherichia coli. Biotechnol. Tech. 6: 133-138.
8. Engler CR (1985). Disruption of microbial cells. In: Moo-Young M, Humphrey AE and Cooney CL (eds) Comprehensive Biotechnology, Vol. 2. Pergmon, Oxford, pp. 305.
9. Engler CD and Robinson CW (1979). New method for measuring cell-wall rupture. Biotech. Bioeng. 21: 1861-1869.
10. Holme T, Arvidsson S, Lindholm B and Pavlu B (1970). Shakeflaskcultivation of bacteria. Process Biochem. 5: 62-66.
11. Kracke-Helm H-A, Brandes L, Hitzmann B, Rinas U and Shügerl K (1991). On-line determination of intracellular β-galactosidase activity in recombinant Escherichia coli using flow injection analysis (FIA). J. Biotechnol. 20: 95-104.
12. Kuboi R, Umakoshi H, Takagi N and Komasawa I (1995). Optimal disruption methods for the selective recovery of β-galactosidase from Escherichia coli. J. Ferment. Bioeng. 79: 335-341.
13. Mattiasson B and Nandakumar MP (1999). Binding assays in heterogenous media using a flow injection system with an expanded bed micro-bed adsorption column. Bioseparation, this volume.
14. Mattiasson B, Svensson K, Borrebaeck C and Kronvall G (1978). Non-equilibrium enzyme immunoassay of gentamycin. Clin. Chem 24: 1770-1773.
15. Miller JM (1972). In Experiments in molecular genetics, pp 352-355. Cold Spring Harbour Laboratory, Cold Spring Harbour, New York.
16. Nandakumar MP, Lali A and Mattiasson B (1999a). On-line monitoring of fermentor using expanded micro-bed flow injection analyser. Bioseparation. (In press).
17. Nandakumar MP, Tocaj A and Mattiasson B (1999b). Analysis of cell containing samples from fermentors using an integrated samples from fermentors using an integrated approach involving sampling, cell desintegration and analysis in a continuous flow injection system containing a micro-expanded enzyme column. Bioseparation, this volume.
18. Neidhardt FC, Ingraham JL and Schaechter M (1990). Physiology of the bacterial cell: A molecular approach. Sinauer Assosiates, Inc. Publishers, Sunderland, Massachusetts.
19. Nilsson M, Håkansson H and Mattiasson B (1992). Evaluation of direct versus competitive binding assays in flow injection systems. Proc. Control Qual. 4: 37-45.
20. Nilsson M, Mattiasson G and Mattiasson B (1993). Automated immunochemical binding assay (flow-ELISA) based on repeated use of an antibody column placed in a flow-injection system. J. Biotechnol. 31: 381-384.
21. Nilsson K and Mosbach K (1980). p-Toluenesulfonyl chloride as an activating agent of agarose for the preparation of immobilized affinity ligands and proteins. Eur. J. Biochem. 112: 397-402.
22. Novella IS, Fargues C and Grévillot G. (1994). Improvement of the extraction of penicillin acylase from Escherichia coli cells by a combined use of chemical methods. Biotechnol. Bioeng 44: 379-382.
23. Pandolfe WD and Kinney R (1983). Recent developments of the understanding of Homogenisation parameters, Paper presented at 1983 Summer National Meeting, AIChE, Denver, CO.
24. Recktenwald A, Kroner K-H and Kula M-R (1985). On-line monitoring of enzymes in downstream processing by flow injection analysis (FIA). Enzyme Microb. Technol. 7: 607-611.
25. Sauer T, Robinson CW and Glick BR (1989). Disruption of native and recombinant Escherichia coli in a high-pressure homogenizer. Biotechnol. Bioeng 33: 1330-1342.
26. Schupp JM, Travis SE, Price LB, Shand RF and Keim P (1995). Rapid bacterial permeabilization reagent useful for enzyme assays. BioTechniques 19: 18-20.
27. Steube K and Spohn U (1994). On-line monitoring of intracellular activities with flow-injection analysis. Anal. Chim. Acta 287: 235-246.
28. Valero F, Lafuente FJ, Solà C, Benito A, Vidal M, Cairó J and Villaverde A (1992). Simultaneous on line monitoring of intracellular β-galactosidase activity and biomass using flow injection analysis in Escherichia coli batch fermentations. Biotech. Technol. 6: 213-218.