Differential inhibitor sensitivity between human recombinant and native photoreceptor cGMP-phosphodiesterases (PDE6s)

Biochemical Pharmacology

Volumn 68, Issue 5, 2004, Pages 867-873

  Zhang, Ji ^a   Kuvelkar, Reshma ^a   Wu, Ping ^a   Egan, Robert W ^a   Billah, M Motasim ^a   Wang, Peng ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

3 isobutyl 1 methylxanthine; Human; IBMX; Inhibitor; PDE; PDE6; phosphodiesterase; Phosphodiesterase; Recombinant; Sildenafil

Indexed keywords

1 [6 CHLORO 4 (3,4 METHYLENEDIOXYBENZYLAMINO) 2 QUINAZOLINYL] 4 PIPERIDINECARBOXYLIC ACID; 2 BROMO 5 ETHYL 7,8 DIHYDRO 1 [(4 HYDROXYPHENYL)METHYL] 7 (PHENYLMETHYL) 1H IMIDAZO[2,1 B]PURIN 4(5H) ONE; 3,7 DIHYDRO 8 [(1 HYDROXYMETHYL) 3 CYCLOPENTEN 1 YL]AMINO 7 [(4 METHOXYPHENYL)METHYL] 1,3 DIMETHYL 1H PURINE 2,6 DIONE; 5 ETHYL 7,8 DIHYDRO 2,7 BIS(PHENYLMETHYL) 1H IMIDAZO[2,1 B]PURIN 4(5H) ONE; 8 BROMO 1 ETHYL 3,7 DIHYDRO 7 [(4 METHOXYPHENYL)METHYL] 3 (2 METHYLPROPYL) 1 H PURINE 2,6 DIONE; CYCLIC GMP PHOSPHODIESTERASE INHIBITOR; IMIDAZOLE DERIVATIVE; ISOBUTYLMETHYLXANTHINE; PHOSPHODIESTERASE 6 INHIBITOR; PHOSPHODIESTERASE INHIBITOR; PHOSPHODIESTERASE V INHIBITOR; PURINE DERIVATIVE; RECOMBINANT ENZYME; SILDENAFIL; UNCLASSIFIED DRUG; ZAPRINAST;

ANIMAL CELL; ARTICLE; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG EFFECT; DRUG POTENCY; DRUG SELECTIVITY; ENZYME ACTIVE SITE; ENZYME INHIBITION; ENZYME SUBUNIT; HUMAN; HUMAN TISSUE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RETINA CONE; RETINA ROD; STATISTICAL SIGNIFICANCE;

3',5'-CYCLIC-GMP PHOSPHODIESTERASE; ANIMALS; CELLS, CULTURED; HUMANS; INSECTS; PHOSPHODIESTERASE INHIBITORS; PHOSPHORIC DIESTER HYDROLASES; PHOTORECEPTORS; PIPERAZINES; RECOMBINANT PROTEINS; TRANSFECTION;

EID: 4043100404     PISSN: 00062952     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bcp.2004.05.015     Document Type: Article

References (41)

1. Beavo J.A. Cyclic nucleotide phosphodiesterases: functional implications of multiple isoforms. Physiol Rev. 75:1995;725-748
2. Chabre M., Deterre P. Molecular mechanism of visual transduction. Eur J Biochem. 179:1989;255-266
3. Yarfitz S., Hurley J.B. Transduction mechanisms of vertebrate and invertebrate photoreceptors. J Biol Chem. 269:1994;14329-14332
4. Baylor D.A., Lamb T.D., Yau K.W. Responses of retinal rods to single photons. J Physiol. 288:1979;613-634
5. Baylor D.A. Photoreceptor signals and vision. Invest Ophthalmol Vis Sci. 28:1987;34-49
6. Normann R.A., Werblin F.S. Control of retinal sensitivity. I. Light and dark adaptation of vertebrate rods and cones. J Gen Physiol. 63:1974;37-61
7. Baehr W., Devlin M.J., Applebury M.L. Isolation and characterization of cGMP phosphodiesteraese from bovine rod outer segments. J Biol Chem. 254:1979;11669-11677
8. Deterre B., Bigay J., Forquet F., Robert M., Chabre M. cGMP phosphodiesterase of retinal rod is regulated by two inhibitory subunits. Proc Natl Acad Sci USA. 85:1988;2424-2428
9. Florio S.K., Prusti R.K., Beavo J.A. Solubilization of membrane-bound rod phosphodiesterase by the rod phosphodiesterase recombinant delta subunit. J Biol Chem. 271:1996;24036-24047
10. Gillespie P.G., Beavo J.A. Characterization of a bovine cone photoreceptor phosphodiesterase purified by cyclic GMP-Sepharose chromatography. J Biol Chem. 263:1988;8133-8141
11. Shimizu-Matsumoto A., Itoh K., Inazawa J., Nishida K., Matsumoto Y., Kinoshita S., et al. Isolation and chromosomal localization of the human cone cGMP phosphodiesterase gamma cDNA (PDE6H). Genomics. 32:1996;121-124
12. Hamilton S.E., Prusti R.K., Bentley J.K., Beavo J.A., Hurley J.B. Affinities of bovine photoreceptor cGMP phosphodiesterasees for rod and cone inhibitory subunits. FEBS Lett. 318:1993;157-161
13. Khramtsov N.Y., Feshchenko E.A., Suslova V.A., Shmukler B.E., Terpugov B.E., Rakitina T.V., et al. The human rod photoreceptor cGMP phosphodiesterase bete-subunit: structural studies of its cDNA and gene. FEBS Lett. 327:1993;275-278
14. Li T., Volppk K., Applebury M.L. Bovine cone photoreceptor cGMP phosphodiesterase structure deduced from a cDNA clone. Proc Natl Acad Sci USA. 87:1990;293-297
15. Ovchinnikov Y.A., Gubanov N.V., Khramtsov K.A., Ischenko V.E., Zagranichny V.E., Muradoe K.G., et al. Cyclic GMP phosphodiesterase from bovine retina: amino acid sequence of the α-subunit and nucleotide sequence of the corresponding cDNA. FEBS Lett. 223:1987;169-173
16. Piriev N.I., Viczian A.S., Ye J., Kerner B., Korenberg J.R., Farber D.B. Gene structure and amino acid sequence of the human cone photoreceptor cGMP-phosphodiesterase alpha' subunit (PDEA2) and its chromosomal localization to 10q24. Genomics. 28:1995;429-435
17. Pittler S.J., Baehr W., Wasmuth J.J., McConnell D.G., Champagne M.S., van Tuinen P., et al. Molecular characterization of human and bovine rod photoreceptor cGMP phosphodiesterases alpha-subunit and chromosomal localization of the human gene. Genomics. 6:1990;272-283
18. Viczian A.S., Piriev N.I., Farber D.E. Isolation and characterization of a CDNA encoding the alpha' subunit of human cone cGMP-phosphodiesterase. Gene. 166:1995;205-211
19. Conti M., Jin S.L.C. The molecular biology of cyclic nucleotide phosphodiesterase. Prog Nucleic Acid Res Mol Biol. 63:2000;1-38
20. Ballard S.A., Gingell C.J., Tang K., Turner L.A., Price M.E., Naylor A.M. Effects of sildenafil on the relaxation of human corpus cavernosum tissue in vitro and on the activities of cyclic nucleotide phosphodiesterase isozymes. J Urol. 159:1998;2164-2171
21. D'Amours M.A., Granovsky A.E., Artemyev N.O., Cote R.H. Potency and mechanism of action of E4021, a type 5 phosphodiesterase isozyme-selective inhibitor, on the photoreceptor phosphodiesterase depend on the state of activation of the enzyme. Mol Pharmacol. 3:1999;508-514
22. Estrade M., Grondin P., Cluzel J., Bonhomme B., Doly M. Effect of a cGMP-specific phosphodiesterase inhibitor on retinal function. Eur J Pharmacol. 352:1998;157-163
23. Saenz de Tejada I., Angulo J., Cuevas P., Fernandez A., Moncada I., Allona A., et al. The phosphodiesterase inhibitory selectivity and the in vitro and in vivo potency of the new PDE5 inhibitor vardenafil. Int J Impot Res. 13:2001;282-290
24. Corbin J.D., Francis S.H., Webb D.J. Phosphodiesterase type 5 as a pharmacologic target in erectile dysfunction. Urol. 60(Suppl. 2B):2002;4-11
25. Burnouf C., Pruniaux M.P. Recent advances in PDE4 inhibitors as immunoregulatory and anti-inflammatory drugs. Curr Pharm Des. 8:2002;1255-1296
26. Corbin J.D., Francis S.H. Pharmacology of phosphodiesterase-5 inhibitors. Int J Clin Pract. 56:2002;453-459
27. Piriev N.I., Yamashita C., Samuel G., Farber D.B. Rod photoreceptor cGMP-phosphodiesterase: analysis of α and β subunits expressed in human kidney cells. Proc Natl Acad Sci USA. 90:1993;9340-9344
28. Piriev N.I., Yamashita C.K., Shih J., Farber D.B. Expression of cone photoreceptor cGMP-phosphodiesterase α′ subunit in Chinese hamster ovary 293 human embryonic kidney and Y79 retinoblastoma cells. Mol Vis. 9:2003;80-86
29. Qin N., Baehr W. Expression and mutagenesis of mouse rod photoreceptor cGMP phosphodiesterase. J Biol Chem. 269:1994;3265-3271
30. Wang P., Wu P., Egan R.W., Billah M.M. Cloning, characterization, and tissue distribution of mouse phosphodiesterase 7A1. Biochem Biophys Res Commun. 276:2000;1271-1277
31. Wang P., Wu P., Egan R.W., Billah M.M. Human phosphodiesterase 8A splice variants: cloning, gene organization, and tissue distribution. Gene. 280:2001;183-194
32. Wang P., Wu P., Myers J.G., Stamford A., Egan R.W., Billah M.M. Characterization of human, dog and rabbit corpus cavernosum type 5 phosphodiesterases. Life Sci. 68:2001;1977-1987
33. Hurwitz R.L., Bunt-Milam A.H., Chang M.L., Beavo J.A. cGMP phosphodiesterase in rod and cone outer segments of the retina. J Biol Chem. 260:1985;568-573
34. Gillespie P.G., Beavo J.A. Inhibition and stimulation of photoreceptor phosphodiesterases by dipyridamole and M&B 22,948. Mol Pharmacol. 36:1989;773-781
35. Vemulapalli S., Watkins R.W., Chintala M., Davis H., Ahn H.S., Fawzi A., et al. Antiplatelet and antiproliferative effects of SCH 51866, a novel type 1 and type 5 hosphodiesterase inhibitor. J Cardiovasc Pharmacol. 170:1996;887-891
36. Wang Y., Chackalamannil S., Hu Z., Boyle C.D., Lankin C.M., Xia Y., et al. Design and synthesis of xanthine analogues as potent and selective PDE5 inhibitors. Bioorg Med Chem Lett. 12:2002;3149-3152
37. Fox D.A., He L., Poblenz A.T., Medrano C.J., Blocker Y.S., Devesh S. Lead-induced alterations in retinal cGMP phosphodiesterase trigger calcium overload, mitochondria dysfunction and rod photoreceptor apoptosis. Toxicol Lett. 102-123:1998;359-361
38. Qiao X., Pennesi M., Seong E., Gao H., Burmeister M., Wu S.M. Photoreceptor degeneration and rd1 mutation in the grizzled/mocha mouse strain. Vision Res. 43:2003;859-865
39. Muradov K.G., Granovsky A.E., Artemyev N.O. Mutation in rod PDE6 linked to congenital stationary night blindness impairs the enzyme inhibition by its gamma-subunit. Biochemistry. 42:2003;3305-3310
40. Michelakis E.D., Tymchak W., Noga M., Webster L., Wu X.C., Lien D., et al. Long-term treatment with oral sildenafil is safe and improves functional capacity and hemodynamics in patients with pulmonary arterial hypertension. Circulation. 108:2003;2066-2069
41. 50) of an enzymatic reaction. Biochem Pharmacol. 22:1973;3099-3108