Generation of nucleophilic character in the Cys25/His159 ion pair of papain involves Trp177 but not Asp158

Biochemistry

Volumn 47, Issue 7, 2008, Pages 2025-2035

  Gul, Sheraz ^a,d   Hussain, Syeed ^a,e   Thomas, Mark P ^a,f   Resmini, Marina ^a   Verma, Chandra S ^b   Thomas, Emrys W ^c   Brocklehurst, Keith ^a  

^a QUEEN MARY UNIVERSITY OF LONDON   (United Kingdom)

^b BIOINFORMATICS INSTITUTE   (Singapore)

^c UNIVERSITY OF SALFORD   (United Kingdom)

^d GLAXOSMITHKLINE   (United Kingdom)

^e ASTRAZENECA   (United Kingdom)

^f CYCLACEL LTD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ACID-CATALYZED ELIMINATION; CATALYTIC COMPETENCE; ION PAIRS;

CATALYSIS; ELECTROSTATICS; NUCLEOPHILES; PH EFFECTS; SOLVATION;

ENZYMES;

2 PYRIDYLDISULFIDE; ASPARTIC ACID; CATION; CYSTEINE; DISULFIDE; HISTIDINE; ION; NITROGEN DERIVATIVE; PAPAIN; PYRIDINE DERIVATIVE; TRYPTOPHAN; UNCLASSIFIED DRUG;

ACIDITY; ALKALINITY; ARTICLE; COMPUTER MODEL; ENZYME ANALYSIS; KINETICS; MOLECULAR PROBE; PH; PRIORITY JOURNAL;

ASPARTIC ACID; CYSTEINE; ELECTROSTATICS; HISTIDINE; MODELS, MOLECULAR; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PAPAIN; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TRYPTOPHAN;

EID: 39649113537     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi702126p     Document Type: Article

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