Protein purification and preliminary crystallographic analysis of human Lyn tyrosine kinase

Protein Expression and Purification

Volumn 58, Issue 2, 2008, Pages 318-324

  Kinoshita, Takayoshi ^a   Miyano, Nao ^a   Nakai, Ryoko ^b   Yokota, Koichi ^b   Ishiguro, Hiroshi ^b   Tada, Toshiji ^a  

^a Osaka Prefecture University   (Japan)

^b 1 5 5 Minatojima Minamimachi   (Japan)

Author keywords

Crystallization; Lyn; Phosphorylation; Src family; Tyrosine kinase

Indexed keywords

LYN PROTEIN-TYROSINE KINASE; PROTEIN KINASE LYN; PROTEIN TYROSINE KINASE; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CHEMISTRY; CRYSTALLIZATION; HUMAN; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; WESTERN BLOTTING; X RAY CRYSTALLOGRAPHY;

BLOTTING, WESTERN; CHROMATOGRAPHY, ION EXCHANGE; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; RECOMBINANT PROTEINS; SRC-FAMILY KINASES;

HEXAPODA;

EID: 39549099724     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2008.01.007     Document Type: Article

References (17)

1. Yamanashi Y., Fukushige S.-I., Semba K., Sukegawa J., Miyajima N., Matsubara K.-I., Yamamoto T., and Toyoshima K. The yes-related cellular gene encodes a possible tyrosine kinase similar to p56lck. Mol. Cell Biol. 7 (1987) 237-243
2. Margaret M.L., and Dunn A.R. Lyn, a Src-like tyrosine kinase. Int. J. Biochem. Cell Biol. 29 (1997) 397-400
3. Suzuki T., Shoji S., Yamamoto K., Nada S., Okada M., Yamamoto T., and Honda X. Essential roles of Lyn in fibronectin-mediated filamentous actin assembly and cell motility in mast cells. J. Immunol. 161 (1998) 3694-3701
4. Lannutti B., and Drachman J.G. Lyn tyrosine kinase regulates thrombopoietin-induced proliferation of hematopoietic cell lines and primary megakaryocytic progenitors. Blood 103 (2004) 3736-3743
5. Simon H.U., Yousefi S., Dibbert B., Hebestreit H., Weber M., Branch D.R., and Blaser K. Role for tyrosine phosphorylation and Lyn tyrosine kinase in fas receptor-mediated apoptosis in eosinophils. Blood 92 (1998) 547-557
6. Chan V.W.F., Meng F., Soriano P., DeFranco A.L., and Lowell C.A. Characterization of the B lymphocyte populations in Lyn-deficient mice and the role of Lyn in signal initiation and down-regulation. Immunity 7 (1997) 69-81
7. Janas M.L., Hdgkin P., Hibbs M., and Tarlinton D. Genetic evidence for Lyn as a negative regulator of IL-4 signaling. J. Immunol. 163 (1999) 4192-4198
8. Harder K.W., Parsons L.M., Armes J., Evans N., Kountouri N., Clark R., Quilici C., Grail D., Hodgson G.S., and Dunn A.R. Gain- and loss-of-function Lyn mutant mice define a critical inhibitory role for Lyn in the myeloid lineage. Immunity 15 (2001) 603-615
9. Xiao W., Nishimoto H., Hong H., Kitaura J., Nunomura S., Maeda-Yamamoto M., Kawakami Y., Lowell C.A., Ra C., and Kawakami T. Positive and negative regulation of mast cell activation by Lyn via the FceRI. J. Immunol. 175 (2005) 6885-6892
10. Schmitz R., Baumann G., and Gram H. Catalytic specificity of phosphotyrosine kinases Blk, Lyn, c-Src, and Syk as assessed by phage display. J. Mol. Biol. 260 (1996) 664-677
11. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72 (1976) 248-254
12. Otwinowski Z., and Minor W. Processing of X-ray Diffraction Data Collected in Oscillation Mode,. Methods in Enzymology, vol. 276: Macromolecular Crystallography, part A (1997), Academic Press, New York pp. 307-326
13. Vagin A., and Teplyakov A. An approach to multi-copy search in molecular replacement. Acta Crystallogr. D56 (2000) 1622-1624
14. The CCP4 suite: programs for protein crystallography. Acta Crystallogr. D50 (1994) 760-763
15. Kinoshita T., Matsubara M., Ishiguro H., Okita K., and Tada T. Structure of human Fyn kinase domain complexed with staurosporine. Biochem. Biophys. Res. Commun. 346 (2006) 840-844
16. Adams P.D., Clore G.M., DeLano W.L., Gros P., Grosse-Kunstleve R.W., Jiang J.-S., Kuszewski J., Nilges M., Pannu N.S., Read R.J., Rice L.M., Simonson T., and Warren G.L. CNS/CSX crystallography & NMR system: a new software suite for macromolecular structure determination. Acta Crystallogr. D54 (1998) 905-921
17. Matthews B.W. Solvent content of protein crystals. J. Mol. Biol. 33 (1968) 491-497