Combination of poly(ethylenimine) and chitosan induces high gene transfection efficiency and low cytotoxicity

Journal of Bioscience and Bioengineering

Volumn 105, Issue 1, 2008, Pages 65-68

  Zhao, Qing Qing ^a   Chen, Jin Liang ^a   Han, Min ^a   Liang, Wen Quan ^a   Tabata, Yasuhiko ^b   Gao, Jian Qing ^a  

^a ZHEJIANG UNIVERSITY   (China)

^b KYOTO UNIVERSITY   (Japan)

Author keywords

chitosan; cytotoxicity; poly(ethylenimine); serum; transfection

Indexed keywords

GENE TRANSFECTION; SERUM; TRANSFECTION;

CYTOTOXICITY; DNA SEQUENCES; GENE EXPRESSION;

CHITOSAN;

LIPOSOME; POLYCATION; POLYETHYLENEIMINE DNA CHITOSAN COMPLEX; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; DRUG CYTOTOXICITY; GENE EXPRESSION; GENETIC TRANSFECTION; HELA CELL; HUMAN; HUMAN CELL; LIPOSOMAL GENE DELIVERY SYSTEM; NONVIRAL GENE DELIVERY SYSTEM; TRANSMISSION ELECTRON MICROSCOPY;

CHITOSAN; DNA; HUMANS; POLYETHYLENEIMINE; TRANSFECTION;

EID: 39449125413     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1263/jbb.105.65     Document Type: Article

References (25)

1. Mansouri S., Lavigne P., Corsi K., Benderdour M., Beaumont E., and Fernandes J.C. Chitosan/DNA nanoparticles as non-viral vectors in gene therapy: strategies to improve transfection efficacy. Eur. J. Pharm. Biopharm. 57 (2004) 1-8
2. Harris J.D., and Lemoine N.R. Strategies for targeted gene therapy. Trends Genet. 12 (1996) 400-405
3. El-Aneed A. An overview of current delivery systems in cancer gene therapy. J. Control. Release 94 (2004) 1-14
4. Cho C.W., Cho Y.S., Kang B.T., Hwang J.S., Park S.N., and Yoon D.Y. Improvement of gene transfer to cervical cancer cell lines using non-viral agents. Cancer Lett. 162 (2001) 75-85
5. Nakamura T., Moriguchi R., Kogure K., Minoura A., Masuda T., Akita H., Kato K., Hamada H., Ueno M., Futaki S., and Harashima H. Delivery of condensed DNA by liposomal non-viral gene delivery system into nucleus of dendritic cells. Biol. Pharm. Bull. 29 (2006) 1290-1293
6. Park M.R., Han K.O., Han I.K., Cho M.H., Nah J.W., Choi Y.J., and Cho C.S. Degradable polyethylenimine-alt-poly(ethylene glycol) copolymers as novel gene carriers. J. Control. Release 105 (2005) 367-380
7. Neu M., Fischer D., and Kissel T. Recent advances in rational gene transfer vector design based on poly(ethyleneimine) and its derivatives. J. Gene Med. 7 (2005) 992-1009
8. Wang J., Zhang P.C., Lu H.F., Ma N., Wang S., Mao H.Q., and Leong K.W. New polyphosphoramidate with a spermidine side chain as a gene carrier. J. Control. Release 83 (2002) 157-168
9. Lee K.Y., Kwon I.C., Kim Y.H., Jo W.H., and Jeong S.Y. Preparation of chitosan self-aggregates as a gene delivery system. J. Control. Release 51 (1998) 213-220
10. Zhang S., Xu Y., Wang B., Qiao W., Liu D., and Li Z. Cationic compounds used in lipoplexes and polyplexes for gene delivery. J. Control. Release 100 (2004) 165-180
11. Yamazaki Y., Nango M., Matsuura M., Hasegawa Y., Hasegawa M., and Oku N. Polycation liposomes, a novel non-viral gene transfer system, constructed from acetylated polyethylenimine. Gene Ther. 7 (2000) 1148-1155
12. Sato A., Kawakami S., Yamada M., Yamashita F., and Hashida M. Enhanced gene trasnfection in macrophages using mannosylated cationic liposome-polyethylenimine-plasmid DNA complexes. J. Drug Target 9 (2001) 201-207
13. Chen J.L., Wang H., Gao J.Q., Chen H.L., and Liang W.Q. Liposomes modified with polycation used for gene delivery: preparation, characterization and transfection in vitro. Int. J. Pharm. 343 (2007) 255-261
14. Zhang X.Q., Wang X.L., Zhang P.C., Liu Z.L., Zhuo R.X., Mao H.Q., and Leong K.W. Galactosylated ternary DNA/polyphophoramidate nanoparticles mediate high gene transfection efficiency in hepatocytes. J. Control. Release 102 (2005) 749-763
15. Kim T.K., Kim S.I., Akaike T., and Cho C.S. Synergistic effect of poly(ethylenimine) on the transfection efficiency of galactosylated chitosan/DNA complexes. J. Control. Release 105 (2005) 354-366
16. Singh R.S., Goncalves C., Sandrin P., Pichon C., Midoux P., and Chaudhuri A. On the gene delivery efficacies of pH-sensitive cationic lipids via endosomal protonation: a chemical biology investigation. Chem. Biol. 11 (2004) 713-723
17. Pack D.W., Hoffman A.S., Pun S., and Stayton P.S. Design and development of polymers for gene delivery. Nat. Rev. Drug. Discov. 4 (2005) 581-593
18. Godbey W.T., Wu K.K., and Mikos A.G. Tracking the intracellular path of poly(ethylenimine)/DNA complexes for gene delivery. Proc. Natl. Acad. Sci. USA 96 (1999) 5177-5181
19. Merdan T., Kunath K., Fischer D., Kopecek J., and Kissel T. Intracellular processing of poly(ethyleneimine)/rybozyme complexes can be observed in living cells by using confocal laser scanning microscopy and inhibitor experiments. Pharm. Res. 19 (2002) 140-146
20. Lee C.H., Ni Y.H., Chen C.C., Chou C.K., and Chang F.H. Synergistic effect of polyethyleminine and cationic liposomes in nucleic acid delivery to human cancer cells. Biochim. Biophys. Acta 1611 (2003) 55-62
21. Sato T., Ishii T., and Okahata Y. In vitro gene delivery mediated by chitosan. Effect of ph, serum, and molecular mass of chitosan on the transfection efficiency. Biomaterials 22 (2001) 2075-2080
22. De Smedt S.C., Demeester J., and Hennink W.E. Cationic polymer based gene delivery systems. Pharm. Res. 17 (2000) 113-126
23. Huang Y.Z., Gao J.Q., Liang W.Q., and Nakagawa S. Preparation and characterization of liposomes encapsulating chitosan nanoparticles. Biol. Pharm. Bull. 28 (2005) 387-390
24. Yamazaki Y., Nango M., Matsuura M., Hasegawa Y., Hasegawa M., and Oku N. Polycation liposomes, a novel non-viral gene transfer system, constructed from cetylated polyethylenimine. Gene Ther. 7 (2000) 1148-1155
25. Garcia L., Bunuales M., Duzgunes N., and Trosde Ilarduya C. Serum-resistant lipopolyplexes for gene delivery to liver tumour cells. Eur. J. Pharm. Biopharm. 67 (2007) 58-66