Coupling homologous recombination with growth selection in yeast: A tool for construction of random DNA sequence libraries

BioTechniques

Volumn 37, Issue 2, 2004, Pages 202-206

  Schaerer Brodbeck, Claudia ^a   Barberis, Alcide ^a  

^a ESBATech AG   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA TOXIN; DNA FRAGMENT; RESTRICTION ENDONUCLEASE; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANTIBODY AFFINITY; CELL DEATH; CELL GROWTH; CELL STRAIN; CELL TRANSFORMATION; COMPLEMENTARITY DETERMINING REGION; CONTROLLED STUDY; DNA LIBRARY; DNA SEQUENCE; FUNGUS; HOMOLOGOUS RECOMBINATION; KLUYVEROMYCES; LETHALITY; MARKER GENE; MOLECULAR CLONING; NONHUMAN; PROTEIN EXPRESSION; REVIEW; TEMPERATURE; YEAST; YEAST CELL;

FUNGI; KLUYVEROMYCES;

EID: 3943091201     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/04372bm05     Document Type: Review

References (16)

1. Sambrook, J., E. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual. CSH Laboratory Press, Cold Spring Harbor, NY.
2. Hua, S.B., Y. Luo, M. Qiu, E. Chan, H. Zhou, and L. Zhu. 1998. Construction of a modular yeast two-hybrid cDNA library from human EST clones for the human genome protein linkage map. Gene 215:143-152.
3. Guthrie, C. and G.R. Fink (Eds.). 1991. Guide to yeast genetics and molecular biology. In Methods in Enzymology. Academic Press, New York.
4. Raymond, C.K., T.A. Pownder, and S.L. Sexson. 1999. General method for plasmid construction using homologous recombination. BioTechniques 26:134-141.
5. Lorenz, M.C., R.S. Muir, E. Lim, J. McElver, S.C. Weber, and J. Heitman. 1995. Gene disruption with PCR products in Saccharomyces cerevisiae. Gene 158:113-117.
6. Yang, W.P., K. Green, S. Pinz-Sweeney, A.T. Briones, D.R. Burton, and C.F. Barbas III. 1995. CDR walking mutagenesis for the affinity maturation of a potent human anti-HIV-1 antibody into the picomolar range. J. Mol. Biol. 254:392-403.
7. Butler, A.R., J.H. White, Y. Folawiyo, A. Edlin, D. Gardiner, and M.J. Stark. 1994. Two Saccharomyces cerevisiae genes which control sensitivity to G1 arrest induced by Kluyveromyces lactis toxin. Mol. Cell. Biol. 14:6306-6316.
8. Magliani, W., S. Conti, M. Gerloni, D. Bertolotti, and L. Polonelli. 1997. Yeast killer systems. Clin. Microbiol. Rev. 10:369-400.
9. Raymond, C.K., E.H. Sims, and M.V. Olson. 2002. Linker-mediated recombinational sub-cloning of large DNA fragments using yeast. Genome Res. 72:190-197.
10. Vidal, M. and P. Legrain. 1999. Yeast forward and reverse 'n'-hybrid systems. Nucleic Acids Res. 27:919-929.
11. Wach, A. 1996. PCR-synthesis of marker cassettes with long flanking homology regions for gene disruptions in S. cerevisiae. Yeast 12:259-265.
12. Barberis, A., J. Pearlberg, N. Simkovich, S. Farrell, P. Reinagel, C. Bamdad, G. Sigal, and M. Ptashne. 1995. Contact with a component of the polymerase II holoenzyme suffices for gene activation. Cell 81:359-368.
13. Woods, R.A. and R.D. Gietz. 2001. High-efficiency transformation of plasmid DNA into yeast. Methods Mol. Biol. 177:85-97.
14. Auf der Maur, A., C. Zahnd, F. Fischer, S. Spinelli, A. Honegger, C. Cambillau, D. Escher, A. Pluckthun, and A. Barberis. 2002. Direct in vivo screening of intrabody libraries constructed on a highly stable single-chain framework. J. Biol. Chem. 277:45075-45085.
15. Boder, E.T. and K.D. Wittrup. 2000. Yeast surface display for directed evolution of protein expression, affinity, and stability. Methods Enzymol. 328:430-444.
16. Urech, D.M., P. Lichtlen, and A. Barberis. 2003. Cell growth selection system to detect extracellular and transmembrane protein interactions. Biochim. Biophys. Acta 1622:117-127.