Development of a 5′-nuclease PCR assay for the identification of Escherichia coli strains expressing the flagellar antigen H21 and their detection in food after enrichment

Journal of Applied Microbiology

Volumn 104, Issue 3, 2008, Pages 899-905

  Auvray, F ^a   Lecureuil, C ^a   Tache J ^a   Perelle, S ^a   Fach, P ^a  

^a Healthy Pigs Production and Experimentation Section   (France)

Author keywords

Escherichia coli; Flagellar antigen; fliC gene; H21; Molecular typing; Real time PCR; Shiga toxin producing Escherichia coli

Indexed keywords

ANTIGENS; GENES; OLIGONUCLEOTIDES; POLYMERASE CHAIN REACTION;

FLAGELLAR ANTIGEN; FLIC GENES; GROUND BEEF; H21; MOLECULAR TYPING; PCR ASSAY; REAL-TIME PCR; REAL-TIME PCR ASSAY; SHIGA TOXIN-PRODUCING E. COLI; SHIGA TOXIN-PRODUCING ESCHERICHIA COLI;

ESCHERICHIA COLI;

BACTERIAL ANTIGEN; H21 ANTIGEN; NUCLEASE; OLIGONUCLEOTIDE; SHIGA TOXIN; UNCLASSIFIED DRUG; ESCHERICHIA COLI PROTEIN; FLIC PROTEIN, E COLI;

ALLELE; ANTIGEN; BIOASSAY; COLIFORM BACTERIUM; DESIGN; DETECTION METHOD; GENE EXPRESSION; IDENTIFICATION METHOD; MOLECULAR ANALYSIS; POLYMERASE CHAIN REACTION; TOXIN;

ALLELE; ANTIGEN EXPRESSION; ARTICLE; BACTERIAL FLAGELLUM; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BEEF; COLONY FORMING UNIT; COMPARATIVE STUDY; ESCHERICHIA COLI; FLIC GENE; FOOD; GENE AMPLIFICATION; GENE SEQUENCE; INOCULATION; MOLECULAR TYPING; NONHUMAN; NUCLEOTIDE SEQUENCE; PATHOTYPE; POLYMERASE CHAIN REACTION; SEROTYPE; ANIMAL; DNA SEQUENCE; FOOD CONTROL; GENETICS; MEAT; METABOLISM; METHODOLOGY; MICROBIOLOGY; MOLECULAR GENETICS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SHIGA TOXIN PRODUCING ESCHERICHIA COLI;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

ANIMALS; BASE SEQUENCE; ESCHERICHIA COLI PROTEINS; FOOD MICROBIOLOGY; MEAT; MOLECULAR SEQUENCE DATA; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; SHIGA-TOXIGENIC ESCHERICHIA COLI;

EID: 39349086607     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2007.03607.x     Document Type: Article

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