Ezrin silencing by small hairpin RNA reverses metastatic behaviors of human breast cancer cells

Cancer Letters

Volumn 261, Issue 1, 2008, Pages 55-63

  Li, Qiong ^a   Wu, Mingfu ^a   Wang, Hui ^b   Xu, Gang ^a   Zhu, Tao ^a   Zhang, Yongtao ^a   Liu, Ping ^a   Song, Anping ^a   Gang, Chen ^a   Han, Zhiqiang ^a   Zhou, Jianfeng ^a   Meng, Li ^a   Lu, Yunpin ^a   Wang, Shixuan ^a   Ma, Ding ^a  

^a HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Breast cancer; Ezrin; Metastasis; RNAi

Indexed keywords

BETA CATENIN; EZRIN; SHORT HAIRPIN RNA; UVOMORULIN;

ARTICLE; BREAST CANCER; CANCER CELL; CELL POLARITY; CONTROLLED STUDY; DOWN REGULATION; GENE SILENCING; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; LYMPH NODE METASTASIS; METASTASIS; ONCOGENE SRC; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA INTERFERENCE; WESTERN BLOTTING; WOUND HEALING;

BETA CATENIN; BREAST NEOPLASMS; CADHERINS; CELL LINE, TUMOR; CELL MOVEMENT; CELL POLARITY; CYTOSKELETAL PROTEINS; DOWN-REGULATION; FEMALE; GENE EXPRESSION REGULATION, NEOPLASTIC; GENES, SRC; HUMANS; NEOPLASM INVASIVENESS; NEOPLASM METASTASIS; PHOSPHORYLATION; RNA INTERFERENCE;

EID: 38949088292     PISSN: 03043835     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.canlet.2007.11.018     Document Type: Article

References (35)

1. Figueroa J.A., Yee D., and McGuire W.L. Prognostic indicators in early breast cancer. Am. J. Med. Sci. 305 (1993) 176-182
2. Jatoi I., Hilsenbeck S.G., Clark G.M., and Osborne C.K. Significance of axillary lymph node metastasis in primary breast cancer. J. Clin. Oncol. 17 (1999) 2334-2340
3. Friedl P., and Wolf K. Tumour-cell invasion and migration: diversity and escape mechanisms. Nat. Rev. Cancer 3 (2003) 362-374
4. Curto M., and McClatchey A.I. Ezrin...a metastatic detERMinant?. Cancer Cell 5 (2004) 113-114
5. Hunter K.W. Ezrin, a key component in tumor metastasis. Trends Mol. Med. 10 (2004) 201-204
6. Elliott B.E., Meens J.A., SenGupta S.K., Louvard D., and Arpin M. The membrane cytoskeletal crosslinker ezrin is required for metastasis of breast carcinoma cells. Breast Cancer Res. 7 (2005) R365-R373
7. Wu M., Bai X., Xu G., Wei J., Zhu T., Zhang Y., Li Q., Liu P., Song A., Zhao L., Gang C., Han Z., Wang S., Zhou J., Lu Y., and Ma D. Proteome analysis of human androgen-independent prostate cancer cell lines: variable metastatic potentials correlated with vimentin expression. Proteomics 7 (2007) 1973-1983
8. Sato N., Funayama N., Nagafuchi A., Yonemura S., Tsukita S., and Tsukita S. A gene family consisting of ezrin, radixin and moesin. Its specific localization at actin filament/plasma membrane association sites. J. Cell Sci. 103 Pt 1 (1992) 131-143
9. Shaw R.J., Henry M., Solomon F., and Jacks T. RhoA-dependent phosphorylation and relocalization of ERM proteins into apical membrane/actin protrusions in fibroblasts. Mol. Biol. Cell. 9 (1998) 403-419
10. Takahashi K., Sasaki T., Mammoto A., Hotta I., Takaishi K., Imamura H., Nakano K., Kodama A., and Takai Y. Interaction of radixin with Rho small G protein GDP/GTP exchange protein Dbl. Oncogene 16 (1998) 3279-3284
11. Gautreau A., Poullet P., Louvard D., and Arpin M. Ezrin, a plasma membrane-microfilament linker, signals cell survival through the phosphatidylinositol 3-kinase/Akt pathway. Proc. Natl. Acad. Sci. USA 96 (1999) 7300-7305
12. Algrain M., Turunen O., Vaheri A., Louvard D., and Arpin M. Ezrin contains cytoskeleton and membrane binding domains accounting for its proposed role as a membrane-cytoskeletal linker. J. Cell Biol. 120 (1993) 129-139
13. Vaheri A., Carpen O., Heiska L., Helander T.S., Jaaskelainen J., Majander-Nordenswan P., Sainio M., Timonen T., and Turunen O. The ezrin protein family: membrane-cytoskeleton interactions and disease associations. Curr. Opin. Cell Biol. 9 (1997) 659-666
14. Yu Y., Khan J., Khanna C., Helman L., Meltzer P.S., and Merlino G. Expression profiling identifies the cytoskeletal organizer ezrin and the developmental homeoprotein Six-1 as key metastatic regulators. Nat. Med. 10 (2004) 175-181
15. Khanna C., Wan X., Bose S., Cassaday R., Olomu O., Mendoza A., Yeung C., Gorlick R., Hewitt S.M., and Helman L.J. The membrane-cytoskeleton linker ezrin is necessary for osteosarcoma metastasis. Nat. Med. 10 (2004) 182-186
16. Makitie T., Carpen O., Vaheri A., and Kivela T. Ezrin as a prognostic indicator and its relationship to tumor characteristics in uveal malignant melanoma. Invest. Ophthalmol. Vis. Sci. 42 (2001) 2442-2449
17. Tokunou M., Niki T., Saitoh Y., Imamura H., Sakamoto M., and Hirohashi S. Altered expression of the ERM proteins in lung adenocarcinoma. Lab. Invest. 80 (2000) 1643-1650
18. Ohtani K., Sakamoto H., Rutherford T., Chen Z., Satoh K., and Naftolin F. Ezrin, a membrane-cytoskeletal linking protein, is involved in the process of invasion of endometrial cancer cells. Cancer Lett. 147 (1999) 31-38
19. Moilanen J., Lassus H., Leminen A., Vaheri A., Butzow R., and Carpen O. Ezrin immunoreactivity in relation to survival in serous ovarian carcinoma patients. Gynecol. Oncol. 90 (2003) 273-281
20. Pujuguet P., Del Maestro L., Gautreau A., Louvard D., and Arpin M. Ezrin regulates E-cadherin-dependent adherens junction assembly through Rac1 activation. Mol. Biol. Cell. 14 (2003) 2181-2191
21. Piedra J., Martinez D., Castano J., Miravet S., Dunach M., and de Herreros A.G. Regulation of beta-catenin structure and activity by tyrosine phosphorylation. J. Biol. Chem. 276 (2001) 20436-20443
22. Xu G., Arregui C., Lilien J., and Balsamo J. PTP1B modulates the association of beta-catenin with N-cadherin through binding to an adjacent and partially overlapping target site. J. Biol. Chem. 277 (2002) 49989-49997
23. Genda T., Sakamoto M., Ichida T., Asakura H., and Hirohashi S. Loss of cell-cell contact is induced by integrin-mediated cell-substratum adhesion in highly-motile and highly-metastatic hepatocellular carcinoma cells. Lab. Invest. 80 (2000) 387-394
24. Menke A., Philippi C., Vogelmann R., Seidel B., Lutz M.P., Adler G., and Wedlich D. Down-regulation of E-cadherin gene expression by collagen type I and type III in pancreatic cancer cell lines. Cancer Res. 61 (2001) 3508-3517
25. Srivastava J., Elliott B.E., Louvard D., and Arpin M. Src-dependent ezrin phosphorylation in adhesion-mediated signaling. Mol. Biol. Cell 16 (2005) 1481-1490
26. Elliott B.E., Qiao H., Louvard D., and Arpin M. Co-operative effect of c-src and ezrin in deregulation of cell-cell contacts and scattering of mammary carcinoma cells. J. Cell. Biochem. 92 (2004) 16-28
27. Q. Li, M.F. Wu, A.P. Song, J.C. Wei, G. Xu, Y.P. Lu, D. Ma, Expression of ezrin and E-cadherin in invasive ductal breast cancer and their correlations to lymphatic metastasis, Ai Zheng 25 (2006) 363-366.
28. Han Z., Xu G., Zhou J., Xing H., Wang S., Wu M., Zhang Y., Lu Y., and Ma D. Inhibition of motile and invasive properties of ovarian cancer cells by ASODN against Rho-associated protein kinase. Cell. Commun. Adhes. 12 (2005) 59-69
29. Bretscher A., Edwards K., and Fehon R.G. ERM proteins and merlin: integrators at the cell cortex. Nat. Rev. Mol. Cell. Biol. 3 (2002) 586-599
30. Louvet-Vallee S. ERM proteins: from cellular architecture to cell signaling. Biol. Cell 92 (2000) 305-316
31. Thomas S.M., and Brugge J.S. Cellular functions regulated by Src family kinases. Annu. Rev. Cell Dev. Biol. 13 (1997) 513-609
32. Abram C.L., and Courtneidge S.A. Src family tyrosine kinases and growth factor signaling. Exp. Cell. Res. 254 (2000) 1-13
33. Takeichi M. Cadherins in cancer: implications for invasion and metastasis. Curr. Opin. Cell Biol. 5 (1993) 806-811
34. Nagafuchi A., Ishihara S., and Tsukita S. The roles of catenins in the cadherin-mediated cell adhesion: functional analysis of E-cadherin-alpha catenin fusion molecules. J. Cell Biol. 127 (1994) 235-245
35. Nam J.S., Yoshinori I., and Michiie S. Src family kinase inhibitor PP2 restores the E-cadherin/catenin cell adhesion system in human cancer cells and reduces cancer metastasis. Clin. Cancer Res. 8 (2002) 2430-2436