DprA/Smf protein localizes at the DNA uptake machinery in competent Bacillus subtilis cells

BMC Microbiology

Volumn 7, Issue , 2007, Pages

  Tadesse, Serkalem ^a,b   Graumann, Peter L ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

^b YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; PLASMID DNA; PROTEIN COMGA; PROTEIN SMF; RECA PROTEIN; RECN PROTEIN; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR COMK; UNCLASSIFIED DRUG; BACTERIAL DNA; COMA PROTEIN, BACTERIA; COMK PROTEIN, BACILLUS SUBTILIS; CYAN FLUORESCENT PROTEIN; DNA BINDING PROTEIN; DPRA PROTEIN, BACTERIA; GREEN FLUORESCENT PROTEIN; MEMBRANE PROTEIN;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL CELL; BACTERIAL STRAIN; CONTROLLED STUDY; GENE DELETION; NONHUMAN; PROTEIN LOCALIZATION; BACTERIUM TRANSFORMATION; CELL MEMBRANE; CHEMISTRY; CYTOPLASM; FLUORESCENCE MICROSCOPY; GENE FUSION; GENETICS; METABOLISM; PHYSIOLOGY; PLASMID; REPORTER GENE;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS);

ARTIFICIAL GENE FUSION; BACILLUS SUBTILIS; BACTERIAL PROTEINS; CELL MEMBRANE; CYTOPLASM; DNA, BACTERIAL; DNA-BINDING PROTEINS; GENE DELETION; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; MEMBRANE PROTEINS; MICROSCOPY, FLUORESCENCE; PLASMIDS; REC A RECOMBINASES; TRANSCRIPTION FACTORS; TRANSFORMATION, BACTERIAL;

EID: 38749106181     PISSN: 14712180     EISSN: 14712180     Source Type: Journal    
DOI: 10.1186/1471-2180-7-105     Document Type: Article

References (27)

1. Genetic competence in Bacillus subtilis. D Dubnau, Microbiol Rev 1991 55 3 395 424 1943994
2. Controlling competence in Bacillus subtilis: shared use of regulators. LW Hamoen G Venema OP Kuipers, Microbiology 2003 149 Pt 1 9 17 10.1099/mic.0.26003-0 12576575
3. Bistability in bacteria. D Dubnau R Losick, Mol Microbiol 2006 61 3 564 572 10.1111/j.1365-2958.2006.05249.x 16879639
4. Bistability in the Bacillus subtilis K-state (competence) system requires a positive feedback loop. H Maamar D Dubnau, Mol Microbiol 2005 56 3 615 624 10.1111/j.1365-2958.2005.04592.x 15819619
5. Stripping Bacillus: ComK auto-stimulation is responsible for the bistable response in competence development. WK Smits CC Eschevins KA Susanna S Bron OP Kuipers LW Hamoen, Mol Microbiol 2005 56 3 604 614 10.1111/j.1365-2958.2005. 04488.x 15819618
6. Microarray analysis of the Bacillus subtilis K-state: genome-wide expression changes dependent on ComK. RM Berka J Hahn M Albano I Draskovic M Persuh X Cui A Sloma W Widner D Dubnau, Mol Microbiol 2002 43 5 1331 1345 10.1046/j.1365-2958.2002.02833.x 11918817
7. Whole-genome analysis of genes regulated by the Bacillus subtilis competence transcription factor ComK. M Ogura H Yamaguchi K Kobayashi N Ogasawara Y Fujita T Tanaka, J Bacteriol 2002 184 9 2344 2351 11948146 10.1128/JB.184.9.2344-2351.2002
8. Improving the predictive value of the competence transcription factor (ComK) binding site in Bacillus subtilis using a genomic approach. LW Hamoen WK Smits A de Jong S Holsappel OP Kuipers, Nucleic Acids Res 2002 30 24 5517 5528 12490720 10.1093/nar/gkf698
9. comK encodes the competence transcription factor, the key regulatory protein for competence development in Bacillus subtilis. D van Sinderen A Luttinger L Kong D Dubnau G Venema L Hamoen, Mol Microbiol 1995 15 3 455 462 10.1111/j.1365-2958.1995.tb02259.x 7783616
10. A ComGA-dependent checkpoint limits growth during the escape from competence. BJ Haijema J Hahn J Haynes D Dubnau, Mol Microbiol 2001 40 1 52 64 10.1046/j.1365-2958.2001.02363.x 11298275
11. The ins and outs of DNA transfer in bacteria. I Chen PJ Christie D Dubnau, Science 2005 310 5753 1456 1460 10.1126/science.1114021 16322448
12. ComEA, a Bacillus subtilis integral membrane protein required for genetic transformation, is needed for both DNA binding and transport. GS Inamine D Dubnau, J Bacteriol 1995 177 11 3045 3051 7768800
13. DNA transport into Bacillus subtilis requires proton motive force to generate large molecular forces. B Maier I Chen D Dubnau MP Sheetz, Nat Struct Mol Biol 2004 11 7 643 649 10.1038/nsmb783 15184891
14. Biogenesis of a putative channel protein, ComEC, required for DNA uptake: membrane topology, oligomerization and formation of disulphide bonds. I Draskovic D Dubnau, Mol Microbiol 2005 55 3 881 896 10.1111/j.1365-2958.2004. 04430.x 15661011
15. Cell surface localization and processing of the ComG proteins, required for DNA binding during transformation of Bacillus subtilis. YS Chung F Breidt D Dubnau, Mol Microbiol 1998 29 3 905 913 10.1046/j.1365-2958.1998.00989.x 9723928
16. Mutation of the putative nucleotide binding site of the Bacillus subtilis membrane protein ComFA abolishes the uptake of DNA during transformation. JA Londono-Vallejo D Dubnau, J Bacteriol 1994 176 15 4642 4645 8045895
17. Transformation proteins and DNA uptake localize to the cell poles in Bacillus subtilis. J Hahn B Maier BJ Haijema M Sheetz D Dubnau, Cell 2005 122 1 59 71 10.1016/j.cell.2005.04.035 16009133
18. Intracellular protein and DNA dynamics in competent Bacillus subtilis cells. D Kidane PL Graumann, Cell 2005 122 1 73 84 10.1016/j.cell.2005.04.036 16009134
19. DNA sequence and characterization of Haemophilus influenzae dprA+, a gene required for chromosomal but not plasmid DNA transformation. S Karudapuram X Zhao GJ Barcak, J Bacteriol 1995 177 11 3235 3240 7768823
20. Role of dprA in transformation of Campylobacter jejuni. T Takata T Ando DA Israel TM Wassenaar MJ Blaser, FEMS Microbiol Lett 2005 252 1 161 168 10.1016/j.femsle.2005.08.052 16194595
21. HP0333, a member of the dprA family, is involved in natural transformation in Helicobacter pylori. T Ando DA Israel K Kusugami MJ Blaser, J Bacteriol 1999 181 18 5572 5580 10482496
22. Transformation of Streptococcus pneumoniae relies on DprA- and RecA-dependent protection of incoming DNA single strands. M Berge I Mortier-Barriere B Martin JP Claverys, Mol Microbiol 2003 50 2 527 536 10.1046/j.1365-2958.2003.03702.x 14617176
23. Fate of transforming DNA following uptake by competent Bacillus subtilis. I. Formation and properties of the donor-recipient complex. D Dubnau R Davidoff-Abelson, J Mol Biol 1971 56 2 209 221 10.1016/0022-2836(71)90460-8 4994568
24. GFP vectors for controlled expression and dual labelling of protein fusions in Bacillus subtilis. PJ Lewis AL Marston, Gene 1999 227 1 101 110 10.1016/S0378-1119(98)00580-0 9931458
25. All seven comG open reading frames are required for DNA binding during transformation of competent Bacillus subtilis. YS Chung D Dubnau, J Bacteriol 1998 180 1 41 45 9422590
26. Characterization of Bacillus subtilis recombinational pathways. JC Alonso G Luder RH Tailor, J Bacteriol 1991 173 13 3977 3980 1905712
27. Dynamic formation of RecA filaments at DNA double strand break repair centers in live cells. D Kidane PL Graumann, J Cell Biol 2005 170 3 357 366 10.1083/jcb.200412090 16061691